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  <front>
    <journal-meta><journal-id journal-id-type="publisher">AMT</journal-id><journal-title-group>
    <journal-title>Atmospheric Measurement Techniques</journal-title>
    <abbrev-journal-title abbrev-type="publisher">AMT</abbrev-journal-title><abbrev-journal-title abbrev-type="nlm-ta">Atmos. Meas. Tech.</abbrev-journal-title>
  </journal-title-group><issn pub-type="epub">1867-8548</issn><publisher>
    <publisher-name>Copernicus Publications</publisher-name>
    <publisher-loc>Göttingen, Germany</publisher-loc>
  </publisher></journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.5194/amt-14-1833-2021</article-id><title-group><article-title>The Roland von Glasow Air-Sea-Ice Chamber (RvG-ASIC):<?xmltex \hack{\break}?> an experimental facility for studying ocean–sea-ice–atmosphere interactions</article-title><alt-title>The Roland von Glasow Air-Sea-Ice Chamber</alt-title>
      </title-group><?xmltex \runningtitle{The Roland von Glasow Air-Sea-Ice Chamber}?><?xmltex \runningauthor{M. Thomas et al.}?>
      <contrib-group>
        <contrib contrib-type="author" corresp="no" rid="aff1 aff2">
          <name><surname>Thomas</surname><given-names>Max</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff1 aff3 aff4">
          <name><surname>France</surname><given-names>James</given-names></name>
          
        <ext-link>https://orcid.org/0000-0002-8785-1240</ext-link></contrib>
        <contrib contrib-type="author" corresp="no" rid="aff1 aff5 aff6">
          <name><surname>Crabeck</surname><given-names>Odile</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff7">
          <name><surname>Hall</surname><given-names>Benjamin</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff8">
          <name><surname>Hof</surname><given-names>Verena</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff8 aff9">
          <name><surname>Notz</surname><given-names>Dirk</given-names></name>
          
        <ext-link>https://orcid.org/0000-0003-0365-5654</ext-link></contrib>
        <contrib contrib-type="author" corresp="no" rid="aff7">
          <name><surname>Rampai</surname><given-names>Tokoloho</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff8">
          <name><surname>Riemenschneider</surname><given-names>Leif</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff1">
          <name><surname>Tooth</surname><given-names>Oliver John</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="no" rid="aff1">
          <name><surname>Tranter</surname><given-names>Mathilde</given-names></name>
          
        </contrib>
        <contrib contrib-type="author" corresp="yes" rid="aff1">
          <name><surname>Kaiser</surname><given-names>Jan</given-names></name>
          <email>j.kaiser@uea.ac.uk</email>
        <ext-link>https://orcid.org/0000-0002-1553-4043</ext-link></contrib>
        <aff id="aff1"><label>1</label><institution>Centre for Ocean and Atmospheric Sciences, School of Environmental Sciences, University of East Anglia,<?xmltex \hack{\break}?> NR4 7TJ, Norwich, UK</institution>
        </aff>
        <aff id="aff2"><label>2</label><institution>Department of Physics, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand</institution>
        </aff>
        <aff id="aff3"><label>3</label><institution>British Antarctic Survey, Natural Environment Research Council, Cambridge CB3 0ET, UK</institution>
        </aff>
        <aff id="aff4"><label>4</label><institution>Department of Earth Sciences, Royal Holloway, University of London, Egham TW20 0EX, UK</institution>
        </aff>
        <aff id="aff5"><label>5</label><institution>Laboratoire de Glaciologie, Université Libre de Bruxelles, Bruxelles, Belgium</institution>
        </aff>
        <aff id="aff6"><label>6</label><institution>Unité d’Océanographie Chimique, Freshwater and Oceanic sCience Unit reSearch (FOCUS),<?xmltex \hack{\break}?> Université de Liége, Liége, Belgium</institution>
        </aff>
        <aff id="aff7"><label>7</label><institution>Chemical Engineering Department, University of Cape Town, Cape Town, South Africa</institution>
        </aff>
        <aff id="aff8"><label>8</label><institution>Max Planck Institute for Meteorology, Hamburg, Germany</institution>
        </aff>
        <aff id="aff9"><label>9</label><institution>Center for Earth System Research and Sustainability (CEN), University of Hamburg, Hamburg, Germany</institution>
        </aff>
      </contrib-group>
      <author-notes><corresp id="corr1">Jan Kaiser (j.kaiser@uea.ac.uk)</corresp></author-notes><pub-date><day>5</day><month>March</month><year>2021</year></pub-date>
      
      <volume>14</volume>
      <issue>3</issue>
      <fpage>1833</fpage><lpage>1849</lpage>
      <history>
        <date date-type="received"><day>29</day><month>September</month><year>2020</year></date>
           <date date-type="rev-request"><day>14</day><month>October</month><year>2020</year></date>
           <date date-type="rev-recd"><day>6</day><month>January</month><year>2021</year></date>
           <date date-type="accepted"><day>27</day><month>January</month><year>2021</year></date>
      </history>
      <permissions>
        <copyright-statement>Copyright: © 2021 Max Thomas et al.</copyright-statement>
        <copyright-year>2021</copyright-year>
      <license license-type="open-access"><license-p>This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">https://creativecommons.org/licenses/by/4.0/</ext-link></license-p></license></permissions><self-uri xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021.html">This article is available from https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021.html</self-uri><self-uri xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021.pdf">The full text article is available as a PDF file from https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021.pdf</self-uri>
      <abstract><title>Abstract</title>
    <p id="d1e233">Sea ice is difficult, expensive, and potentially dangerous to observe in nature. The remoteness of the Arctic Ocean and Southern Ocean complicates sampling logistics, while the heterogeneous nature of sea ice and rapidly changing environmental conditions present challenges for conducting process studies. Here, we describe the Roland von Glasow Air-Sea-Ice Chamber (RvG-ASIC), a laboratory facility designed to reproduce polar processes and overcome some of these challenges. The RvG-ASIC is an open-topped 3.5 m<inline-formula><mml:math id="M1" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:math></inline-formula> glass tank housed in a cold room (temperature range: <inline-formula><mml:math id="M2" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">55</mml:mn></mml:mrow></mml:math></inline-formula> to <inline-formula><mml:math id="M3" display="inline"><mml:mrow><mml:mo>+</mml:mo><mml:mn mathvariant="normal">30</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M4" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C). The RvG-ASIC is equipped with a wide suite of instruments for ocean, sea ice, and atmospheric measurements, as well as visible and UV lighting. The infrastructure, available instruments, and typical experimental protocols are described.</p>
    <p id="d1e274">To characterise some of the technical capabilities of our facility, we have quantified the timescale over which our chamber exchanges gas with the outside, <inline-formula><mml:math id="M5" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">l</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">0.66</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.07</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> d, and the mixing rate of our experimental ocean, <inline-formula><mml:math id="M6" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">4.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> min. Characterising our light field, we show that the light intensity across the tank varies by less than 10 % near the centre of the tank but drops to as low as 60 % of the maximum intensity in one corner. The temperature sensitivity of our light sources over the 400 to 700 nm range (PAR) is <inline-formula><mml:math id="M7" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mn mathvariant="normal">0.028</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.003</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> W m<inline-formula><mml:math id="M8" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M9" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C<inline-formula><mml:math id="M10" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>, with a maximum irradiance of 26.4 W m<inline-formula><mml:math id="M11" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> at 0 <inline-formula><mml:math id="M12" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C; over the 320 to 380 nm range, it is <inline-formula><mml:math id="M13" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mn mathvariant="normal">0.16</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> W m<inline-formula><mml:math id="M14" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M15" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C<inline-formula><mml:math id="M16" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>, with a maximum irradiance of 5.6 W m<inline-formula><mml:math id="M17" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> at 0 <inline-formula><mml:math id="M18" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C.</p>
    <p id="d1e465">We also present results characterising our experimental sea ice. The extinction coefficient for PAR varies from 3.7 to 6.1 m<inline-formula><mml:math id="M19" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> when calculated from irradiance measurements exterior to the sea ice and from 4.4 to 6.2 m<inline-formula><mml:math id="M20" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> when calculated from irradiance measurements within the sea ice. The bulk salinity of our experimental sea ice is measured using three techniques, modelled using a halo-dynamic one-dimensional (1D) gravity drainage model, and calculated from a salt and mass budget. The growth rate of our sea ice is between 2 and 4 cm d<inline-formula><mml:math id="M21" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for air temperatures of <inline-formula><mml:math id="M22" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mo>-</mml:mo><mml:mn mathvariant="normal">9.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M23" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C and <inline-formula><mml:math id="M24" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mo>-</mml:mo><mml:mn mathvariant="normal">26.6</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M25" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C. The PAR extinction coefficients, vertically integrated bulk salinities, and growth rates all lie within the range of previously reported comparable values for first-year sea ice. The vertically integrated bulk salinity and growth<?pagebreak page1834?> rates can be reproduced well by a 1D model. Taken together, the similarities between our laboratory sea ice and observations in nature, as well as our ability to reproduce our results with a model, give us confidence that sea ice grown in the RvG-ASIC is a good representation of natural sea ice.</p>
  </abstract>
    </article-meta>
  </front>
<body>
      

<sec id="Ch1.S1" sec-type="intro">
  <label>1</label><title>Introduction</title>
      <p id="d1e568">Sea ice lies at the ocean–atmosphere interface. As such, sea ice mediates the exchange of energy <xref ref-type="bibr" rid="bib1.bibx13" id="paren.1"><named-content content-type="pre">e.g.</named-content></xref>, momentum <xref ref-type="bibr" rid="bib1.bibx30" id="paren.2"><named-content content-type="pre">e.g.</named-content></xref>, gases <xref ref-type="bibr" rid="bib1.bibx12" id="paren.3"><named-content content-type="pre">e.g.</named-content></xref>, and particles <xref ref-type="bibr" rid="bib1.bibx28" id="paren.4"><named-content content-type="pre">e.g.</named-content></xref> between the polar oceans and the atmosphere. Sea-ice formation provides buoyancy forcing to the underlying ocean <xref ref-type="bibr" rid="bib1.bibx59" id="paren.5"/>. Sea-ice algae inhabit brine inclusions in the sea ice and can reach high concentrations, with nutrients resupplied by gravity drainage <xref ref-type="bibr" rid="bib1.bibx10" id="paren.6"/>.</p>
      <p id="d1e598">The remoteness and extremeness typical of the polar oceans make observing sea ice in situ difficult, expensive, and potentially dangerous – both for personnel and equipment. Such logistical challenges are heightened during the initial growth and final melt of sea ice, which are particularly interesting study periods. Also, the heterogeneous nature of sea ice, with important parameters varying over sub-metre scales <xref ref-type="bibr" rid="bib1.bibx32" id="paren.7"/> and responding to sub-diurnal fluctuations in air temperature, make conducting process studies in the field difficult. These logistical and scientific difficulties motivate the use of laboratory-grown sea ice to bridge observational gaps.</p>
      <p id="d1e604">Existing laboratory facilities vary widely, tending to be designed with specific observations in mind and circumventing specific constraints. The lengths of sea-ice tanks vary from tens of metres <xref ref-type="bibr" rid="bib1.bibx43 bib1.bibx2" id="paren.8"><named-content content-type="pre">e.g.</named-content></xref> to tens of centimetres <xref ref-type="bibr" rid="bib1.bibx9 bib1.bibx35" id="paren.9"><named-content content-type="pre">e.g.</named-content></xref>. Larger tanks minimise edge effects and allow for more samples to be taken. Smaller tanks are cheaper to build and run and have better controlled boundary conditions. The cooling methods used to form artificial sea ice range from cold plates <xref ref-type="bibr" rid="bib1.bibx3 bib1.bibx9 bib1.bibx58" id="paren.10"/> through the controlled atmosphere of a cold room <xref ref-type="bibr" rid="bib1.bibx50 bib1.bibx46 bib1.bibx33 bib1.bibx27" id="paren.11"/> to the outdoors <xref ref-type="bibr" rid="bib1.bibx43 bib1.bibx16" id="paren.12"/>. Cold plates provide the tightest control over the upper boundary condition but preclude the study of many processes at the upper interface, because the experimental system has no atmosphere. There are few chambers in the literature with atmospheres enclosed in a headspace <xref ref-type="bibr" rid="bib1.bibx35" id="paren.13"/>, possibly because, as noted by <xref ref-type="bibr" rid="bib1.bibx25" id="text.14"/>, the temperature of an enclosed headspace tends to be much warmer than the cold room in which the experimental system is housed. Most facilities use the entire cold room as an atmosphere. Tanks have been made of glass <xref ref-type="bibr" rid="bib1.bibx33" id="paren.15"/>, plastic <xref ref-type="bibr" rid="bib1.bibx24 bib1.bibx9" id="paren.16"/>, stainless steel <xref ref-type="bibr" rid="bib1.bibx44" id="paren.17"/>, and concrete <xref ref-type="bibr" rid="bib1.bibx43" id="paren.18"/>. Of these, glass has the advantage of excellent chemical inertness, while steel, concrete, and plastic are cheaper and can be more robust. Glass and plastic allow the sea ice to be observed through the tank sides. Lighting may be placed above a sea-ice tank to provide illumination for radiative transfer experiments <xref ref-type="bibr" rid="bib1.bibx40 bib1.bibx27" id="paren.19"/>. Finally, sea-ice tanks are either cuboid <xref ref-type="bibr" rid="bib1.bibx50 bib1.bibx33 bib1.bibx43 bib1.bibx35 bib1.bibx58" id="paren.20"><named-content content-type="pre">e.g.</named-content></xref>, cylindrical <xref ref-type="bibr" rid="bib1.bibx3 bib1.bibx40 bib1.bibx24 bib1.bibx27" id="paren.21"><named-content content-type="pre">e.g.</named-content></xref>, or quasi two-dimensional Hele-Shaw cells <xref ref-type="bibr" rid="bib1.bibx9 bib1.bibx31" id="paren.22"/>. Wave generation is easier in a cuboid tank, whereas cylindrical tanks will have simpler edge effects. Hele-Shaw cells allow for visualisation of the internal sea-ice structure.</p>
      <p id="d1e662">There are several examples of sea-ice tanks having proved effective in generating and testing hypotheses. As an example, much of our understanding of gravity drainage was produced or refined using sea-ice tank experiments. Laboratory studies traced brine <xref ref-type="bibr" rid="bib1.bibx3 bib1.bibx9" id="paren.23"/>, visualised brine channels <xref ref-type="bibr" rid="bib1.bibx34" id="paren.24"/>, and provided idealised conditions to evaluate models <xref ref-type="bibr" rid="bib1.bibx58 bib1.bibx36" id="paren.25"/> and measurement techniques <xref ref-type="bibr" rid="bib1.bibx39" id="paren.26"/>. The understanding, qualitative and quantitative, of processes developed by these studies has led to the development of physically faithful, precise gravity drainage parameterisations <xref ref-type="bibr" rid="bib1.bibx57 bib1.bibx14 bib1.bibx51 bib1.bibx42" id="paren.27"/>. Sea-ice tanks also played a key role in developing models of gas transport in and around sea ice. Laboratory experiments have been used to estimate the magnitude of gas fluxes <xref ref-type="bibr" rid="bib1.bibx35" id="paren.28"/>, investigate the processes by which gases are transported <xref ref-type="bibr" rid="bib1.bibx24 bib1.bibx22 bib1.bibx44" id="paren.29"/>, and to investigate ocean–atmosphere gas fluxes through breaks in sea-ice cover <xref ref-type="bibr" rid="bib1.bibx25 bib1.bibx26" id="paren.30"/>. For both gas fluxes and gravity drainage, progress was made by integrating laboratory studies with field measurements and modelling <xref ref-type="bibr" rid="bib1.bibx61 bib1.bibx37" id="paren.31"><named-content content-type="pre">e.g.</named-content></xref>. Laboratory sea ice has helped develop our understanding of radiative transfer in sea ice <xref ref-type="bibr" rid="bib1.bibx40 bib1.bibx27" id="paren.32"/>, frost flower formation <xref ref-type="bibr" rid="bib1.bibx46 bib1.bibx41" id="paren.33"/>, the thermodynamics of grease ice and nilas <xref ref-type="bibr" rid="bib1.bibx33 bib1.bibx5" id="paren.34"/>, and many other fields.</p>
      <p id="d1e706">Here, we describe and characterise the Roland von Glasow Air-Sea-Ice Chamber (RvG-ASIC) to aid future users when planning experiments. We first describe the facility, the instrumentation available, and typical protocols used to grow artificial sea ice (Sect. <xref ref-type="sec" rid="Ch1.S2"/>). Next, we characterise the air exchange rate of the chamber with the outside, the mixing rate of the tank, and the variability of the light field – important<?pagebreak page1835?> technical metrics for designing and interpreting experiments (Sect. <xref ref-type="sec" rid="Ch1.S3.SS1"/>). We then evaluate how similar our sea ice is to natural sea ice by comparing our experimental sea ice to natural sea ice for three key parameters: the extinction of photosynthetically active radiation, the bulk salinity, and the growth rate (Sect. <xref ref-type="sec" rid="Ch1.S3.SS2"/>).</p>
</sec>
<sec id="Ch1.S2">
  <label>2</label><title>Facility description</title>
<sec id="Ch1.S2.SS1">
  <label>2.1</label><title>Infrastructure</title>
      <p id="d1e730">Our artificial ocean is contained in a cuboid, open-topped, glass tank (Fig. <xref ref-type="fig" rid="Ch1.F1"/>). The internal footprint of the tank is 2.35 m <inline-formula><mml:math id="M26" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 1.35 m, and the depth of the tank is 1.17 m. The glass is 25 mm thick, joined at the edges with silicone resin, and reinforced with a metal bracketing bar. A smaller cuboid glass tank (0.5 m <inline-formula><mml:math id="M27" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 0.4 m footprint, 1.12 m depth, 12 mm wall thickness) is joined to the main tank, connected by four 100 mm holes (Fig. <xref ref-type="fig" rid="Ch1.F1"/>). This side tank – capped with a lid – is never allowed to freeze over entirely and so provides a path for sample lines into the ocean, a path for cables that does not interfere with the sea-ice–atmosphere interface, and a free path for water displaced by volume expansion upon freezing. The main and side tanks have been insulated using 10 cm of Dow 500A Floormate foam (0.035 W m<inline-formula><mml:math id="M28" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> K<inline-formula><mml:math id="M29" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> thermal conductivity) and 10 cm of foil-backed loft insulation. Heating film (12 V, maximum output 220 W m<inline-formula><mml:math id="M30" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>) was placed outside of the glass and within the insulation. The heating pads and insulation ensure that the dominant cooling of our experimental system is at the exposed upper interface rather than through the tank sides. They also prevent sea ice attaching to our tank walls so that it remains free floating. Two to four pumps (TUNZE stream 6125, maximum flow rate of 12 000 L h<inline-formula><mml:math id="M31" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>) are placed in the main and side tanks to mix the ocean. The pumps are fixed to the tank walls using magnets and can be positioned as needed.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F1"><?xmltex \currentcnt{1}?><?xmltex \def\figurename{Figure}?><label>Figure 1</label><caption><p id="d1e802">The tank just after installation <bold>(a)</bold>, with all the main features in place <bold>(b)</bold>, and set up for experiments with visible lighting <bold>(c)</bold>. The labels are consistent with Fig. <xref ref-type="fig" rid="Ch1.F3"/>, indicating: a the main tank, b the side tank, c the lights, e atmosphere sample lines and cables, f ocean sample lines and cables, and i the (0, 0) position of our sampling coordinate system.</p></caption>
          <?xmltex \igopts{width=199.169291pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f01.png"/>

        </fig>

      <p id="d1e822">A lighting rack sits at 1.3 m above the tank base (Fig. <xref ref-type="fig" rid="Ch1.F1"/>). Eight UV-B lamps (Philips broadband TL 100 W), eight UV-A lamps (Philips Cleo performance 100 W), and eight visible LED strips (Fluence solar max) are spaced evenly over the length of the tank. The visible lights can be individually dimmed from 100 % to 10 % of the full output. The LED, UV-A, and UV-B spectra are shown in Fig. <xref ref-type="fig" rid="Ch1.F2"/>.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F2"><?xmltex \currentcnt{2}?><?xmltex \def\figurename{Figure}?><label>Figure 2</label><caption><p id="d1e832">Spectral irradiance, <inline-formula><mml:math id="M32" display="inline"><mml:mrow><mml:msub><mml:mi>E</mml:mi><mml:mi mathvariant="italic">λ</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, for the lights in the RvG-ASIC at 1 m height and room temperature.</p></caption>
          <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f02.png"/>

        </fig>

      <p id="d1e852">The tank and lights are housed in a cold room with a temperature range of <inline-formula><mml:math id="M33" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">55</mml:mn></mml:mrow></mml:math></inline-formula> to <inline-formula><mml:math id="M34" display="inline"><mml:mrow><mml:mo>+</mml:mo><mml:mn mathvariant="normal">30</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M35" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C. The external footprint of the cold room is 4.32 m <inline-formula><mml:math id="M36" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 4.32 m. The external height is 3.15 m and the internal height is 2.85 m (Fig. <xref ref-type="fig" rid="Ch1.F3"/>). The internal surfaces of the cold room are stainless steel. Six fans recirculate air within the cold room via a compressor. The cold room is connected to the outside by double doors, with six 10 cm holes for sample, power, and data lines; one drainage hole; and one ventilation hole. All of these can be closed, and the sample, power, and data lines can be fed in through gas-tight Roxtec ports. The cold room is controlled by a Eurotherm nanodac control panel.</p>

      <fig id="Ch1.F3" specific-use="star"><?xmltex \currentcnt{3}?><?xmltex \def\figurename{Figure}?><label>Figure 3</label><caption><p id="d1e894">Scaled schematic diagram of the cold room. The three panels show orthogonal views from different vantage points. Crosses and dots indicate air flow away from and towards the viewer, respectively. The lights, shown in grey, are made up of eight sets of visible, UV-A, and UV-B triplets. The main and side tanks are pale green.</p></caption>
          <?xmltex \igopts{width=398.338583pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f03.png"/>

        </fig>

      <?pagebreak page1836?><p id="d1e903">The cold room is located in an external laboratory. The control boxes for instrumentation, heating, pumps, cameras, and valves are all situated adjacent to the cold room. Instruments log to an Envidas Ultimate acquisition system software for continuous monitoring of the data. Mains or 18.2 M<inline-formula><mml:math id="M37" display="inline"><mml:mi mathvariant="normal">Ω</mml:mi></mml:math></inline-formula> water supplies (Centra R 200) are available for filling the tank.</p>
      <p id="d1e913">We use “chamber” to describe the experimental system. When the tank is exposed to the entire cold room, as for all the experiments presented here, the cold room is the chamber.</p>
</sec>
<sec id="Ch1.S2.SS2">
  <label>2.2</label><title>Instrumentation</title>
      <p id="d1e924">The RvG-ASIC has a suite of instruments for carrying out measurements of the experimental ocean, sea ice, and atmosphere (Table <xref ref-type="table" rid="Ch1.T1"/>).</p>

<table-wrap id="Ch1.T1" specific-use="star"><?xmltex \currentcnt{1}?><label>Table 1</label><caption><p id="d1e931">Instruments that are generally available in the RvG-ASIC.</p></caption><oasis:table frame="topbot"><?xmltex \begin{scaleboxenv}{.90}[.90]?><oasis:tgroup cols="3">
     <oasis:colspec colnum="1" colname="col1" align="justify" colwidth="6cm"/>
     <oasis:colspec colnum="2" colname="col2" align="justify" colwidth="6.5cm"/>
     <oasis:colspec colnum="3" colname="col3" align="justify" colwidth="5cm"/>
     <oasis:thead>
       <oasis:row rowsep="1">
         <oasis:entry colname="col1">Instrument</oasis:entry>
         <oasis:entry colname="col2">Parameter</oasis:entry>
         <oasis:entry colname="col3">Experiment compartment</oasis:entry>
       </oasis:row>
     </oasis:thead>
     <oasis:tbody>
       <oasis:row>
         <oasis:entry colname="col1">Sea-Bird MicroCAT SBE 37-SIP</oasis:entry>
         <oasis:entry colname="col2">conductivity, temperature</oasis:entry>
         <oasis:entry colname="col3">ocean</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">DS18B20U digital thermometer</oasis:entry>
         <oasis:entry colname="col2">temperature</oasis:entry>
         <oasis:entry colname="col3">sea ice and ocean</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Wireharp</oasis:entry>
         <oasis:entry colname="col2">liquid fraction</oasis:entry>
         <oasis:entry colname="col3">sea ice</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">TCS3472 photodiodes</oasis:entry>
         <oasis:entry colname="col2">irradiance, 400 to 700 nm</oasis:entry>
         <oasis:entry colname="col3">sea ice, ocean, and atmosphere</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Ocean Optics spectrometer (USB2000<inline-formula><mml:math id="M38" display="inline"><mml:mo>+</mml:mo></mml:math></inline-formula>)</oasis:entry>
         <oasis:entry colname="col2">spectral irradiance</oasis:entry>
         <oasis:entry colname="col3">sea ice, ocean, and atmosphere</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Metcon spectral radiometer</oasis:entry>
         <oasis:entry colname="col2">wavelength-resolved 280 to 650 nm actinic flux</oasis:entry>
         <oasis:entry colname="col3">sea ice, ocean, and atmosphere</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">LGR GGA-30r-EP</oasis:entry>
         <oasis:entry colname="col2">CO<inline-formula><mml:math id="M39" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula> and CH<inline-formula><mml:math id="M40" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula> mole fraction</oasis:entry>
         <oasis:entry colname="col3">atmosphere and equilibrated air</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">LGR FGGA</oasis:entry>
         <oasis:entry colname="col2">CO<inline-formula><mml:math id="M41" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula> and CH<inline-formula><mml:math id="M42" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula> mole fraction</oasis:entry>
         <oasis:entry colname="col3">atmosphere and equilibrated air</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Teledyne T200UP</oasis:entry>
         <oasis:entry colname="col2">NO <inline-formula><mml:math id="M43" display="inline"><mml:mo>/</mml:mo></mml:math></inline-formula> NO<inline-formula><mml:math id="M44" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula> <inline-formula><mml:math id="M45" display="inline"><mml:mo>/</mml:mo></mml:math></inline-formula> NO<inline-formula><mml:math id="M46" display="inline"><mml:msub><mml:mi/><mml:mi>x</mml:mi></mml:msub></mml:math></inline-formula> mole fractions</oasis:entry>
         <oasis:entry colname="col3">atmosphere</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Teledyne T200U</oasis:entry>
         <oasis:entry colname="col2">NO <inline-formula><mml:math id="M47" display="inline"><mml:mo>/</mml:mo></mml:math></inline-formula> NO<inline-formula><mml:math id="M48" display="inline"><mml:msub><mml:mi/><mml:mi>y</mml:mi></mml:msub></mml:math></inline-formula> mole fractions</oasis:entry>
         <oasis:entry colname="col3">atmosphere</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Teledyne T400</oasis:entry>
         <oasis:entry colname="col2">O<inline-formula><mml:math id="M49" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msub></mml:math></inline-formula> mole fraction</oasis:entry>
         <oasis:entry colname="col3">atmosphere</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Teledyne T700U</oasis:entry>
         <oasis:entry colname="col2">dynamic dilution calibrator with ozone generator</oasis:entry>
         <oasis:entry colname="col3"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Teledyne T701H</oasis:entry>
         <oasis:entry colname="col2">zero air generator</oasis:entry>
         <oasis:entry colname="col3"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">WS600-UMB</oasis:entry>
         <oasis:entry colname="col2">temperature, wind speed, humidity</oasis:entry>
         <oasis:entry colname="col3">atmosphere</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Camsecure, underwater camera</oasis:entry>
         <oasis:entry colname="col2">video</oasis:entry>
         <oasis:entry colname="col3">ocean</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">4K HD video camera</oasis:entry>
         <oasis:entry colname="col2">video</oasis:entry>
         <oasis:entry colname="col3">atmosphere</oasis:entry>
       </oasis:row>
     </oasis:tbody>
   </oasis:tgroup><?xmltex \end{scaleboxenv}?></oasis:table></table-wrap>

<sec id="Ch1.S2.SS2.SSS1">
  <label>2.2.1</label><title>Ocean</title>
      <p id="d1e1262">The ocean temperature, <inline-formula><mml:math id="M50" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">θ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, and conductivity, <inline-formula><mml:math id="M51" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, can be monitored by a temperature and conductivity recorder (Sea-Bird MicroCAT SBE 37SIP or Valeport mini CT). <inline-formula><mml:math id="M52" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">θ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M53" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> measurements are converted to a salinity, <inline-formula><mml:math id="M54" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> (g kg<inline-formula><mml:math id="M55" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>), using either the GSW toolbox <xref ref-type="bibr" rid="bib1.bibx29" id="paren.35"/> for representative ocean salt composition or using equations presented in <xref ref-type="bibr" rid="bib1.bibx33" id="text.36"/> for pure NaCl. A sonar (Aquascat 1000R) measures the position of the waterline at the start of the experiment and the position of the base of the sea ice throughout the experiment. We use the GSW toolbox to calculate the speed of sound for a given <inline-formula><mml:math id="M56" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M57" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">θ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> and scale the raw sonar output to produce an accurate distance measurement. We can deploy a Liquicel 1.7 <inline-formula><mml:math id="M58" display="inline"><mml:mo>×</mml:mo></mml:math></inline-formula> 5.5 MiniModule in our ocean coupled to a LGR GGA-30r-EP OA-ICOS laser spectrometer to measure the concentration of dissolved CH<inline-formula><mml:math id="M59" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula> and CO<inline-formula><mml:math id="M60" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula> in the ocean without removing water from the experimental system.</p>
</sec>
<sec id="Ch1.S2.SS2.SSS2">
  <label>2.2.2</label><title>Sea ice</title>
      <p id="d1e1395">Temperature, <inline-formula><mml:math id="M61" display="inline"><mml:mi mathvariant="italic">θ</mml:mi></mml:math></inline-formula>, profiles through the ocean and sea ice are measured using chains of digital thermometers (Table <xref ref-type="table" rid="Ch1.T1"/>). These have a resolution of <inline-formula><mml:math id="M62" display="inline"><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mn mathvariant="normal">1</mml:mn><mml:mn mathvariant="normal">16</mml:mn></mml:mfrac></mml:mstyle></mml:math></inline-formula> <inline-formula><mml:math id="M63" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C and are calibrated against <inline-formula><mml:math id="M64" display="inline"><mml:mi mathvariant="italic">θ</mml:mi></mml:math></inline-formula> measured by the Sea-Bird 37SIP before each run while they sit in well-mixed water. The chains have 1 to 8 cm resolution and are 10 to 80 cm long. Wireharps <xref ref-type="bibr" rid="bib1.bibx39 bib1.bibx37" id="paren.37"/> are used to measure a liquid fraction profile, <inline-formula><mml:math id="M65" display="inline"><mml:mi mathvariant="italic">ϕ</mml:mi></mml:math></inline-formula>, through the sea ice using
              <disp-formula id="Ch1.E1" content-type="numbered"><label>1</label><mml:math id="M66" display="block"><mml:mrow><mml:mi mathvariant="italic">ϕ</mml:mi><mml:mo>=</mml:mo><mml:mstyle displaystyle="true"><mml:mfrac style="display"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:msub><mml:mi>R</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi>t</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>,</mml:mo><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:msub><mml:mi>R</mml:mi><mml:mi>t</mml:mi></mml:msub></mml:mrow></mml:mfrac></mml:mstyle><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where <inline-formula><mml:math id="M67" display="inline"><mml:mi>R</mml:mi></mml:math></inline-formula> is the resistance between a given wire pair, <inline-formula><mml:math id="M68" display="inline"><mml:mi mathvariant="italic">γ</mml:mi></mml:math></inline-formula> is the conductivity of the solution between the wire pairs, and the subscripts 0 and <inline-formula><mml:math id="M69" display="inline"><mml:mi>t</mml:mi></mml:math></inline-formula> indicate the point in time at which the sea-ice front passes a wire pair and some later point in time. We calculate the bulk sea-ice salinity, <inline-formula><mml:math id="M70" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> (g kg<inline-formula><mml:math id="M71" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>), using
              <disp-formula id="Ch1.E2" content-type="numbered"><label>2</label><mml:math id="M72" display="block"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mi mathvariant="italic">ϕ</mml:mi><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>)</mml:mo><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where <inline-formula><mml:math id="M73" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> is a the brine salinity. <inline-formula><mml:math id="M74" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> is retrieved from the third-order polynomial presented in <xref ref-type="bibr" rid="bib1.bibx53" id="text.38"/> (for natural seawater composition)
              <disp-formula id="Ch1.E3" content-type="numbered"><label>3</label><mml:math id="M75" display="block"><mml:mtable rowspacing="0.2ex" columnspacing="1em" class="split" displaystyle="true" columnalign="right left"><mml:mtr><mml:mtd><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>/</mml:mo><mml:mo>(</mml:mo><mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">g</mml:mi><mml:mspace linebreak="nobreak" width="0.125em"/><mml:mi mathvariant="normal">kg</mml:mi></mml:mrow><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup><mml:mo>)</mml:mo></mml:mrow></mml:mtd><mml:mtd><mml:mrow><mml:mo>=</mml:mo><mml:mo>-</mml:mo><mml:mn mathvariant="normal">18.7</mml:mn><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>/</mml:mo><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">C</mml:mi></mml:mrow><mml:mo>)</mml:mo><mml:mo>-</mml:mo><mml:mn mathvariant="normal">0.519</mml:mn><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>/</mml:mo><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">C</mml:mi></mml:mrow><mml:msup><mml:mo>)</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:mrow></mml:mtd></mml:mtr><mml:mtr><mml:mtd/><mml:mtd><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">0.00535</mml:mn><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>/</mml:mo><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">C</mml:mi></mml:mrow><mml:msup><mml:mo>)</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:mrow></mml:mtd></mml:mtr></mml:mtable></mml:math></disp-formula>
            or <xref ref-type="bibr" rid="bib1.bibx42" id="text.39"/> (for NaCl, fit to the data of <xref ref-type="bibr" rid="bib1.bibx55" id="altparen.40"/>),
              <disp-formula id="Ch1.E4" content-type="numbered"><label>4</label><mml:math id="M76" display="block"><mml:mtable columnspacing="1em" class="split" rowspacing="0.2ex" displaystyle="true" columnalign="right left"><mml:mtr><mml:mtd><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>/</mml:mo><mml:mo>(</mml:mo><mml:msup><mml:mtext>g kg</mml:mtext><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup><mml:mo>)</mml:mo></mml:mrow></mml:mtd><mml:mtd><mml:mrow><mml:mo>=</mml:mo><mml:mo>-</mml:mo><mml:mn mathvariant="normal">17.6</mml:mn><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>/</mml:mo><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">C</mml:mi></mml:mrow><mml:mo>)</mml:mo><mml:mo>-</mml:mo><mml:mn mathvariant="normal">0.389</mml:mn><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>/</mml:mo><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">C</mml:mi></mml:mrow><mml:msup><mml:mo>)</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:mrow></mml:mtd></mml:mtr><mml:mtr><mml:mtd/><mml:mtd><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">0.00362</mml:mn><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>/</mml:mo><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">C</mml:mi></mml:mrow><mml:msup><mml:mo>)</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:msup><mml:mo>.</mml:mo></mml:mrow></mml:mtd></mml:mtr></mml:mtable></mml:math></disp-formula></p>
      <p id="d1e1816">Our version of the wireharps use two alternating current frequencies, 2 kHz (as used in <xref ref-type="bibr" rid="bib1.bibx39" id="altparen.41"/>) and 16 kHz. When using the 2 kHz channel, we calculated <inline-formula><mml:math id="M77" display="inline"><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi>t</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>,</mml:mo><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:mfrac></mml:mstyle></mml:math></inline-formula> following <xref ref-type="bibr" rid="bib1.bibx39" id="text.42"/>. When using the 16 kHz channel, we presume that the electronic double layers driving the conductivity changes are not present and so take <inline-formula><mml:math id="M78" display="inline"><mml:mrow><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi>t</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:mi mathvariant="italic">θ</mml:mi><mml:mo>,</mml:mo><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:mfrac></mml:mstyle><mml:mo>=</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:math></inline-formula>.
<?xmltex \hack{\break}?>We have also deployed fibre optics and photodiode light sensors <xref ref-type="bibr" rid="bib1.bibx17" id="paren.43"><named-content content-type="post">Table <xref ref-type="table" rid="Ch1.T1"/></named-content></xref> in the sea ice and ocean. Fibre optics can be connected to a spectrometer outside of the cold room (Ocean Optics USB2000<inline-formula><mml:math id="M79" display="inline"><mml:mo>+</mml:mo></mml:math></inline-formula>). The light sensors consist of photodiodes, waterproofed in resin, measuring in the blue, green, red, and clear. The wavelength range integrated by the photodiodes is, to a good approximation, 400 to 700 nm. The response of the photodiodes is negligible to light with wavelengths shorter than 400 nm. At wavelengths above 700 nm, the response is generally less than 10 % of the maximum response. Diffusing glass plates sit atop the photodiodes to increase the angle of incidence at which light reaches the diodes. The data sheet for the photodiodes is given in the Supplement.</p>
</sec>
<?pagebreak page1838?><sec id="Ch1.S2.SS2.SSS3">
  <label>2.2.3</label><title>Atmosphere</title>
      <p id="d1e1919">The temperature, wind speed, and relative humidity of our atmosphere are measured using a weather station (WS600-UMB). Two Los Gatos Research (LGR) greenhouse gas analysers measure CO<inline-formula><mml:math id="M80" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula>, CH<inline-formula><mml:math id="M81" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula>, and H<inline-formula><mml:math id="M82" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula>O mole fractions in the atmosphere and can also analyse the air stream of the Liquicel equilibrator (Table <xref ref-type="table" rid="Ch1.T1"/>). A Teledyne T200UP measures NO and NO<inline-formula><mml:math id="M83" display="inline"><mml:msub><mml:mi/><mml:mi>x</mml:mi></mml:msub></mml:math></inline-formula> and a T200U measures NO and NO<inline-formula><mml:math id="M84" display="inline"><mml:msub><mml:mi/><mml:mi>y</mml:mi></mml:msub></mml:math></inline-formula>. A Teledyne T400 measures ozone. There is also a zero air generator (T701 H, Teledyne) and a T700U dynamic dilution calibrator with ozone generator.</p>
</sec>
</sec>
<sec id="Ch1.S2.SS3">
  <label>2.3</label><title>Experimental protocols</title>
      <p id="d1e1979">Protocols vary widely between experiments. Here, we provide a typical protocol to help future users visualise the facility and plan experiments.</p>
<sec id="Ch1.S2.SS3.SSS1">
  <label>2.3.1</label><title>Set-up phase</title>
      <p id="d1e1989">We set up instrumentation in a dry, clean tank. Sea-ice instrumentation is attached to poles that are free to rise in the vertical as sea ice grows and floats. Ocean and atmosphere instrumentation and sample lines are mounted in a fixed position. The nature of the experiment will determine the state of the tank sides and base. For optical experiments (Sect. <xref ref-type="sec" rid="Ch1.S3.SS2.SSS1"/>), the tank's inner surfaces should be covered to simplify the light field. We use mirrored sides and a matt black base to approximate infinite lateral boundary conditions and a non-reflective ocean (Fig. <xref ref-type="fig" rid="Ch1.F1"/>).</p>
      <p id="d1e1996">To fill the tank, we add 100 kg of salt and mix this with tap or 18.2 M<inline-formula><mml:math id="M85" display="inline"><mml:mi mathvariant="normal">Ω</mml:mi></mml:math></inline-formula> water. The salt can be pure NaCl (used for Sect. <xref ref-type="sec" rid="Ch1.S3.SS1.SSS2"/> and <xref ref-type="sec" rid="Ch1.S3.SS2.SSS1"/>) or a natural sea salt composition (Tropic Marin sea salt classic, used for Sect. <xref ref-type="sec" rid="Ch1.S3.SS2.SSS2"/>). The tank takes around 18 h to fill with de-ionised water or a few hours from the tap, and 100 kg of salt dissolves in a few hours under vigorous circulation by the pumps. Alternatively, real sea water can be delivered to the tank. Filling the tank to 1 m gives an ocean volume of around 3.2 m<inline-formula><mml:math id="M86" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:math></inline-formula>. Once the tank is full, we begin to cool the water. The tank surface can be covered at this stage to reduce evaporation. With an atmospheric temperature of <inline-formula><mml:math id="M87" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">20</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M88" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C, and an uncovered tank, the temperature of 3.2 m<inline-formula><mml:math id="M89" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:math></inline-formula> of ocean drops by around 1 <inline-formula><mml:math id="M90" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C every 4 h. If the experiment requires an isolated cold room atmosphere, the cold room is sealed at this stage. Sea-ice growth can be initialised by continuing to cool under constant temperature and vigorous circulation. Alternatively, once the water is within a few tenths of a degree of its freezing point, turning the pumps to a low setting, or off, initialises sea-ice growth within a few minutes. As a rule of thumb, it takes one full week to go from a dry tank to first sea ice.</p>
</sec>
<sec id="Ch1.S2.SS3.SSS2">
  <label>2.3.2</label><title>Growth phase</title>
      <p id="d1e2067">The water circulation rate and the atmospheric temperature determine the nature of the sea ice <xref ref-type="bibr" rid="bib1.bibx33" id="paren.44"/>. Nilas will grow in quiescent water. In circulating water, a layer of grease ice will form that subsequently consolidates. When the growth temperature is less than <inline-formula><mml:math id="M91" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">25</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M92" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C, frost flowers form on the sea-ice surface (Fig. <xref ref-type="fig" rid="Ch1.F4"/>). By running the heating pads between the main tank and insulation, we are able to maintain free-floating sea ice to 20 cm thickness with no visible water gap at the tank sides. We have verified that the sea ice is free-floating in several experimental runs by gently pressing the corner of the sea ice and noting that it bobs. With no, or insufficient, side heating, the sea ice attaches to the tank sides and the surface floods <xref ref-type="bibr" rid="bib1.bibx43" id="paren.45"><named-content content-type="pre">e.g.</named-content></xref>, resulting in a shiny, liquid surface layer. Sea ice, fast to the tank walls, has been grown up to 25 cm thickness and could potentially be grown thicker. With insufficient side heating and insulation, the ocean tends to supercool (Fig. <xref ref-type="fig" rid="Ch1.F5"/>). When the ocean supercools, ice grows on the tank sides and instruments, causing problems with measurements and potentially damaging equipment. Growth phases typically last for a few days to a few weeks.</p>

      <?xmltex \floatpos{p}?><fig id="Ch1.F4"><?xmltex \currentcnt{4}?><?xmltex \def\figurename{Figure}?><label>Figure 4</label><caption><p id="d1e2104">Grease ice <bold>(a)</bold> grown under pumping, nilas <bold>(b)</bold> grown in quiescent conditions, and a frost flower field <bold>(c)</bold> grown in the RvG-ASIC.</p></caption>
            <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f04.png"/>

          </fig>

      <?xmltex \floatpos{t}?><fig id="Ch1.F5"><?xmltex \currentcnt{5}?><?xmltex \def\figurename{Figure}?><label>Figure 5</label><caption><p id="d1e2124">A view of a supercooled ocean. Frazil ice crystals are floating upwards in the water column (white flecks) and have nucleated on wireharps well ahead of the advancing sea-ice–ocean interface.</p></caption>
            <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f05.png"/>

          </fig>

</sec>
<sec id="Ch1.S2.SS3.SSS3">
  <label>2.3.3</label><title>Sampling protocols</title>
      <p id="d1e2141">Underlying water may be sampled throughout, providing the volume is replaced. We sample sea ice either by taking cores using a 7.5 cm diameter Kovacs ice corer or using the procedures outlined by <xref ref-type="bibr" rid="bib1.bibx2" id="text.46"/> to extract sea-ice “slabs”. Sea-ice cores have a known bias, particularly problematic in young sea ice, as brine is lost from the permeable sea ice near the ocean interface. The method of <xref ref-type="bibr" rid="bib1.bibx2" id="text.47"/> seeks to minimise this bias by collecting sea-ice samples that are still floating and then freezing the sample and surrounding ocean at <inline-formula><mml:math id="M93" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">40</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M94" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C to immobilise the brine before processing the sample. The method of <xref ref-type="bibr" rid="bib1.bibx2" id="text.48"/> becomes difficult and time consuming when sea ice is thicker than around 25 cm due to limitations of the power tools used to extract the slabs and the weight of the slabs. We cut cores and slabs into discrete vertical profiles using a bandsaw with a precleaned blade at <inline-formula><mml:math id="M95" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">25</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M96" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C.</p>
</sec>
</sec>
</sec>
<sec id="Ch1.S3">
  <label>3</label><title>Characterisation of experimental system</title>
      <p id="d1e2202">We now turn to a characterisation of our experimental system. First, in Sect. <xref ref-type="sec" rid="Ch1.S3.SS1"/>, we quantify several parameters regarding the technical capabilities of the facility: the exchange rate between the chamber and the outside, the mixing rate of the water in the tank, and the variability in the light field. In doing so, we aim at providing valuable information to help plan and interpret future studies. Next, in Sect. <xref ref-type="sec" rid="Ch1.S3.SS2"/>, we present measurements of our experimental sea ice to quantify the extinction of PAR (<inline-formula><mml:math id="M97" display="inline"><mml:mrow><mml:mi mathvariant="italic">λ</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">400</mml:mn></mml:mrow></mml:math></inline-formula> to 700 nm), the bulk salinity, and the growth rate of our experimental sea ice. The goal<?pagebreak page1839?> of this section is to investigate how similar our laboratory-grown sea ice is to natural sea ice with respect to these important characteristics.</p>
<sec id="Ch1.S3.SS1">
  <label>3.1</label><title>Technical characterisation</title>
<sec id="Ch1.S3.SS1.SSS1">
  <label>3.1.1</label><title>Quantifying the cold room air exchange rate</title>
      <p id="d1e2235">For most experiments, it is desirable to have the sea-ice–atmosphere interface exposed to the bulk air within the cold room. Leaving the atmosphere of the tank uncapped ensures that the temperature of the atmosphere overlying the sea ice is responsive to the cold room atmosphere. When the atmosphere is contained by some headspace, the temperature tends to be much warmer than the cold room <xref ref-type="bibr" rid="bib1.bibx25" id="paren.49"><named-content content-type="pre">e.g.</named-content></xref>. Here, we quantify the degree to which the cold room can be sealed from the outside by deriving the air exchange rate coefficient, <inline-formula><mml:math id="M98" display="inline"><mml:mi>k</mml:mi></mml:math></inline-formula>, and time constant, <inline-formula><mml:math id="M99" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">l</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, of CH<inline-formula><mml:math id="M100" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula> exchanging between the cold room to the outside (Fig. <xref ref-type="fig" rid="Ch1.F6"/>).</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F6"><?xmltex \currentcnt{6}?><?xmltex \def\figurename{Figure}?><label>Figure 6</label><caption><p id="d1e2274">Data from experiments to determine the chamber air exchange rate. Panels <bold>(a)</bold> to <bold>(c)</bold> and <bold>(d)</bold> to <bold>(f)</bold> show data from the first and second experiments, respectively. The top panels (<bold>a</bold> and <bold>d</bold>) show the measured CH<inline-formula><mml:math id="M101" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula> concentrations in the chamber and the lab outside. Panels <bold>(b)</bold> and <bold>(e)</bold> show the concentration difference between the chamber and the lab outside. Panels <bold>(c)</bold> and <bold>(f)</bold> show the linear regressions constructed using Eq. (<xref ref-type="disp-formula" rid="Ch1.E5"/>). Blue dots show individual data points. The red lines show the best fit and 95 % confidence intervals of the linear regression of <inline-formula><mml:math id="M102" display="inline"><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:mi>t</mml:mi></mml:mrow></mml:mfrac></mml:mstyle></mml:math></inline-formula> against <inline-formula><mml:math id="M103" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub><mml:mo>-</mml:mo><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">room</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. The gradient of the linear regression corresponds to the air exchange rate coefficient, <inline-formula><mml:math id="M104" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mi>k</mml:mi></mml:mrow></mml:math></inline-formula>. For the first experiment, <inline-formula><mml:math id="M105" display="inline"><mml:mrow><mml:mi>k</mml:mi><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">1.5</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.3</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> d<inline-formula><mml:math id="M106" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>, and for the second experiment, <inline-formula><mml:math id="M107" display="inline"><mml:mrow><mml:mi>k</mml:mi><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">1.5</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> d<inline-formula><mml:math id="M108" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>. </p></caption>
            <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f06.png"/>

          </fig>

      <p id="d1e2439">To do so, we sealed the cold room and left the tank dry. We then diluted the chamber air by filling it with N<inline-formula><mml:math id="M109" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula> gas. We performed two dilutions, the first at around 18:00 LT
on 12 February 2019 and the other at around 16:00 LT on 13 February 2019. The CH<inline-formula><mml:math id="M110" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula> concentration in the chamber, <inline-formula><mml:math id="M111" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, was monitored using an LGR (GGA type, Table <xref ref-type="table" rid="Ch1.T1"/>), where measured air was returned to the cold room in a closed loop (Fig. <xref ref-type="fig" rid="Ch1.F6"/>a and d). The dilution reduced <inline-formula><mml:math id="M112" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> by around 0.1 ppm in both dilution periods. We stopped the dilution by stopping the N<inline-formula><mml:math id="M113" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msub></mml:math></inline-formula> flow, at which point the chamber was sealed to the best of our ability. We measured the CH<inline-formula><mml:math id="M114" display="inline"><mml:msub><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msub></mml:math></inline-formula> concentration in the outer laboratory, <inline-formula><mml:math id="M115" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">room</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, throughout using an LGR (FGGA type, Table <xref ref-type="table" rid="Ch1.T1"/>), and we used the difference between <inline-formula><mml:math id="M116" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M117" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">room</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> as the concentration gradient between the chamber and the outside (Fig. <xref ref-type="fig" rid="Ch1.F6"/>b and e). Before the<?pagebreak page1840?> experiment, the two LGR instruments measured the same air for 30 h. The offset between them was <inline-formula><mml:math id="M118" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mn mathvariant="normal">6.4</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.2</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> ppb. This offset was added to <inline-formula><mml:math id="M119" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">room</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> values to make the measurements from the two LGR instruments consistent. Data were averaged in 5 min bins. We modelled the exchange rate as a first-order process,
              <disp-formula id="Ch1.E5" content-type="numbered"><label>5</label><mml:math id="M120" display="block"><mml:mrow><mml:mstyle displaystyle="true"><mml:mfrac style="display"><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:mi>t</mml:mi></mml:mrow></mml:mfrac></mml:mstyle><mml:mo>=</mml:mo><mml:mo>-</mml:mo><mml:mi>k</mml:mi><mml:mo>(</mml:mo><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub><mml:mo>-</mml:mo><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">room</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mo>.</mml:mo></mml:mrow></mml:math></disp-formula>
            We approximate <inline-formula><mml:math id="M121" display="inline"><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:mi>t</mml:mi></mml:mrow></mml:mfrac></mml:mstyle></mml:math></inline-formula> for each measurement interval using the difference in <inline-formula><mml:math id="M122" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> between adjacent data points over the 5 min between those data. Panels c (experiment 1) and f (experiment 2) of Fig. <xref ref-type="fig" rid="Ch1.F6"/> show the relationship between <inline-formula><mml:math id="M123" display="inline"><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub></mml:mrow><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:mi>t</mml:mi></mml:mrow></mml:mfrac></mml:mstyle></mml:math></inline-formula> and <inline-formula><mml:math id="M124" display="inline"><mml:mrow><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">cham</mml:mi></mml:msub><mml:mo>-</mml:mo><mml:msub><mml:mi>C</mml:mi><mml:mi mathvariant="normal">room</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, as well as the linear fit. We derived <inline-formula><mml:math id="M125" display="inline"><mml:mrow><mml:mi>k</mml:mi><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">1.5</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.3</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> d<inline-formula><mml:math id="M126" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> and <inline-formula><mml:math id="M127" display="inline"><mml:mrow><mml:mi>k</mml:mi><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">1.5</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> d<inline-formula><mml:math id="M128" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for the first and second experiments, respectively. Taking the mean <inline-formula><mml:math id="M129" display="inline"><mml:mi>k</mml:mi></mml:math></inline-formula> of the two experiments, the air exchange time constant is then given by
              <disp-formula id="Ch1.E6" content-type="numbered"><label>6</label><mml:math id="M130" display="block"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">l</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mn mathvariant="normal">1</mml:mn><mml:mo>/</mml:mo><mml:mi>k</mml:mi><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">0.66</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.07</mml:mn><mml:mo>)</mml:mo><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mi mathvariant="normal">d</mml:mi><mml:mo>.</mml:mo></mml:mrow></mml:math></disp-formula>
            The physical interpretation of this <inline-formula><mml:math id="M131" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">l</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is that the concentration difference between the sealed chamber and the outside will reduce by a factor of <inline-formula><mml:math id="M132" display="inline"><mml:mi>e</mml:mi></mml:math></inline-formula> every <inline-formula><mml:math id="M133" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mn mathvariant="normal">0.66</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.07</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> d.</p>
</sec>
<sec id="Ch1.S3.SS1.SSS2">
  <label>3.1.2</label><title>Quantifying the tank mixing rate</title>
      <p id="d1e2839">For some experiments, it may be necessary to spike the ocean with a chemical. We may, in such cases, want to know when the water will be well mixed with respect to our spike. Similar to the air exchange rate (Sect. <xref ref-type="sec" rid="Ch1.S3.SS1.SSS1"/>), the degree to which the chemical has mixed can be quantified using a time constant, <inline-formula><mml:math id="M134" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. To derive this time constant, we spiked our tank with saturated NaCl solution by injecting it near the tank base and observed the conductivity of our ocean over time as it mixed under the action of three pumps (Fig. <xref ref-type="fig" rid="Ch1.F7"/>). The temperature of the ocean was stable to within 0.02 <inline-formula><mml:math id="M135" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C for the hour time period over which we monitored both spikes, so we expect the salinity to be linearly related to the conductivity. The conductivity of the ocean, <inline-formula><mml:math id="M136" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, can then be described by
              <disp-formula id="Ch1.E7" content-type="numbered"><label>7</label><mml:math id="M137" display="block"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:mi>t</mml:mi><mml:mo>)</mml:mo><mml:mo>=</mml:mo><mml:msup><mml:mi>e</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mstyle scriptlevel="+1"><mml:mfrac><mml:mi>t</mml:mi><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub></mml:mrow></mml:mfrac></mml:mstyle></mml:mrow></mml:msup><mml:mo>(</mml:mo><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:mo>)</mml:mo><mml:mo>-</mml:mo><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mrow><mml:mtext>o</mml:mtext><mml:mo>,</mml:mo><mml:mi mathvariant="normal">∞</mml:mi></mml:mrow></mml:msub><mml:mo>)</mml:mo><mml:mo>+</mml:mo><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mrow><mml:mtext>o</mml:mtext><mml:mo>,</mml:mo><mml:mi mathvariant="normal">∞</mml:mi></mml:mrow></mml:msub><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where <inline-formula><mml:math id="M138" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">γ</mml:mi><mml:mrow><mml:mtext>o</mml:mtext><mml:mo>,</mml:mo><mml:mi mathvariant="normal">∞</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math></inline-formula> is the maximum ocean conductivity obtained after the spike and <inline-formula><mml:math id="M139" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> is the time of the spike. Fitting an exponential curve to a manually defined spike period for the two runs produces <inline-formula><mml:math id="M140" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">4.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> min (run 1) and <inline-formula><mml:math id="M141" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">4.1</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> min (run 2). We take the mean of the individual experiments to characterise our mixing rate, such that <inline-formula><mml:math id="M142" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">4.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> min. The tank should therefore mix to more than 99% of the perfectly mixed concentration in less than 30 min.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F7"><?xmltex \currentcnt{7}?><?xmltex \def\figurename{Figure}?><label>Figure 7</label><caption><p id="d1e3056">Experiments to determine the mixing time constant of our tank, <inline-formula><mml:math id="M143" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. Saturated NaCl solution was spiked into the tank and mixed under pumping. The conductivity of the bulk water is shown by the grey markers. The coloured lines show the prediction of Eq. (<xref ref-type="disp-formula" rid="Ch1.E7"/>) using <inline-formula><mml:math id="M144" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">τ</mml:mi><mml:mi mathvariant="normal">m</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> fit to the data. In reality, the spike in run 2 was a few hours after run 1, but the times for the two spikes have been matched for ease of comparison.</p></caption>
            <?xmltex \igopts{width=199.169291pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f07.png"/>

          </fig>

</sec>
<sec id="Ch1.S3.SS1.SSS3">
  <label>3.1.3</label><title>Quantifying light-field variability</title>
      <p id="d1e3097">Producing a homogeneous light field in a laboratory environment can be difficult, because shading and reflection can cause heterogeneities. The intensity of our lights is also temperature dependent, which is particularly important in the RvG-ASIC given the wide range of experimental temperatures. In this section, we aim to characterise our light field, both in terms of lateral heterogeneity and with changes in temperature.</p>
      <?pagebreak page1841?><p id="d1e3100">To assess the spatial variability, we measured PAR (photosynthetically active radiation, <inline-formula><mml:math id="M145" display="inline"><mml:mrow><mml:mi mathvariant="italic">λ</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">400</mml:mn></mml:mrow></mml:math></inline-formula> to 700 nm) across the tank area at 1 m height (Fig. <xref ref-type="fig" rid="Ch1.F8"/>). These measurements were made using a LI-COR LI-1800 spectroradiometer integrated from 400 to 700 nm. The PAR irradiance, <inline-formula><mml:math id="M146" display="inline"><mml:mrow><mml:msub><mml:mi>I</mml:mi><mml:mi mathvariant="normal">PAR</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, at 1 m above the tank base and 25 <inline-formula><mml:math id="M147" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C was generally within 80 % of the maximum recorded intensity, <inline-formula><mml:math id="M148" display="inline"><mml:mrow><mml:msub><mml:mi>I</mml:mi><mml:mrow><mml:mi mathvariant="normal">PAR</mml:mi><mml:mo>,</mml:mo><mml:mi mathvariant="normal">max</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math></inline-formula>, but dropped as low as 60 % in one corner of the tank.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F8"><?xmltex \currentcnt{8}?><?xmltex \def\figurename{Figure}?><label>Figure 8</label><caption><p id="d1e3155">Normalised PAR (<inline-formula><mml:math id="M149" display="inline"><mml:mrow><mml:mi mathvariant="italic">λ</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">400</mml:mn></mml:mrow></mml:math></inline-formula> to 700 nm) irradiance across the footprint of the tank at 1 m height. The (0, 0) cm coordinate corresponds to the pink asterisk in Fig. <xref ref-type="fig" rid="Ch1.F3"/>.</p></caption>
            <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f08.png"/>

          </fig>

      <p id="d1e3179">We measured spectra for UV (UV-A lighting, 320 to 380 nm) and PAR (LED lighting, 400 to 700 nm) at <inline-formula><mml:math id="M150" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">30</mml:mn></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M151" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">20</mml:mn></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M152" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">10</mml:mn></mml:mrow></mml:math></inline-formula>, and 0 <inline-formula><mml:math id="M153" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C using a USB2000<inline-formula><mml:math id="M154" display="inline"><mml:mo>+</mml:mo></mml:math></inline-formula> spectrometer referenced against the Metcon spectral radiometer (Fig. <xref ref-type="fig" rid="Ch1.F9"/>). To quantify the temperature sensitivity, we integrated the UV and PAR spectral irradiance over their respective wavelength ranges (320 to 380 nm and 400 to 700 nm, respectively). The maximum irradiance was 5.6 W m<inline-formula><mml:math id="M155" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for UV and 26.4 W m<inline-formula><mml:math id="M156" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for PAR, both at 0 <inline-formula><mml:math id="M157" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C. The temperature sensitivity of the irradiance was (<inline-formula><mml:math id="M158" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.16</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.01</mml:mn></mml:mrow></mml:math></inline-formula>) W m<inline-formula><mml:math id="M159" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M160" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C<inline-formula><mml:math id="M161" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for UV and (<inline-formula><mml:math id="M162" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.028</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.003</mml:mn></mml:mrow></mml:math></inline-formula>) W m<inline-formula><mml:math id="M163" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M164" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C<inline-formula><mml:math id="M165" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for PAR. The UV-A lights are more strongly temperature dependent, with irradiance dropping by 83 % between 0 and <inline-formula><mml:math id="M166" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">30</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M167" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C, while PAR irradiance dropped by just 3 %.</p>

      <fig id="Ch1.F9" specific-use="star"><?xmltex \currentcnt{9}?><?xmltex \def\figurename{Figure}?><label>Figure 9</label><caption><p id="d1e3376">Sensitivity of lights to variations in temperature.  Panels <bold>(a)</bold> (UV) and <bold>(c)</bold> (PAR) show spectra taken at four temperatures between 0 and <inline-formula><mml:math id="M168" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">30</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M169" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C. Panels <bold>(b)</bold> (UV, 320 to 380 nm) and <bold>(d)</bold> (PAR, 400 to 700 nm) show the irradiance, <inline-formula><mml:math id="M170" display="inline"><mml:mi>I</mml:mi></mml:math></inline-formula> (integrated spectral irradiance over the respective wavelength ranges), at each temperature, <inline-formula><mml:math id="M171" display="inline"><mml:mi mathvariant="italic">θ</mml:mi></mml:math></inline-formula>. The gradient of the regression of <inline-formula><mml:math id="M172" display="inline"><mml:mi>I</mml:mi></mml:math></inline-formula> against <inline-formula><mml:math id="M173" display="inline"><mml:mi mathvariant="italic">θ</mml:mi></mml:math></inline-formula> is (<inline-formula><mml:math id="M174" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.028</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.003</mml:mn></mml:mrow></mml:math></inline-formula>) W m<inline-formula><mml:math id="M175" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M176" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C<inline-formula><mml:math id="M177" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for PAR and (<inline-formula><mml:math id="M178" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.16</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.01</mml:mn></mml:mrow></mml:math></inline-formula>) W m<inline-formula><mml:math id="M179" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M180" display="inline"><mml:msup><mml:mi/><mml:mi>o</mml:mi></mml:msup></mml:math></inline-formula>C<inline-formula><mml:math id="M181" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> for UV.</p></caption>
            <?xmltex \igopts{width=341.433071pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f09.png"/>

          </fig>

</sec>
</sec>
<sec id="Ch1.S3.SS2">
  <label>3.2</label><title>Sea-ice characterisation</title>
<sec id="Ch1.S3.SS2.SSS1">
  <label>3.2.1</label><title>Determining PAR extinction coefficients in sea ice</title>
      <p id="d1e3553">The importance of understanding the sea-ice light field is critical as thinner, fresher, and more transient sea ice becomes more common. Even thin sea ice without snow cover impacts the transmission of PAR to the ocean and has been seen to accumulate algal biomass rapidly within the sea ice <xref ref-type="bibr" rid="bib1.bibx47" id="paren.50"/>. Photochemical production rates within sea ice are also dependent upon sea-ice optical properties. Irradiation through sea ice has been postulated as a source of OH radicals <xref ref-type="bibr" rid="bib1.bibx21" id="paren.51"/> and as a stressor of diatoms that may lead to iodine production from algae <xref ref-type="bibr" rid="bib1.bibx23" id="paren.52"/>.</p>
      <p id="d1e3565">The rationale for UV–Vis illumination experiments in a controlled sea-ice facility is twofold: first, to allow for experiments investigating the optical properties of sea ice (and potentially other mediums such as snow); second, to allow simple experimental simulations of photochemistry or biology occurring in the sea ice, atmosphere, or ocean. The extinction coefficient, <inline-formula><mml:math id="M182" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula>, of PAR in sea ice quantifies the rate of attenuation of PAR with depth. Here, we present measurements of <inline-formula><mml:math id="M183" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> for sea ice grown in the RvG-ASIC and compare those to previously reported values.</p>
      <?pagebreak page1842?><p id="d1e3582">To quantify <inline-formula><mml:math id="M184" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> in our experimental sea ice, we performed an experiment using visible lighting, measuring a vertical profile of irradiance using eight photodiodes (Sect. <xref ref-type="sec" rid="Ch1.S2.SS2.SSS2"/>). The photodiodes respond dominantly to 400 to 700 nm light, and our lighting provides irradiance predominantly in the 400 to 700 nm range, so <inline-formula><mml:math id="M185" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> as measured by the photodiodes is a good estimate of <inline-formula><mml:math id="M186" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> for PAR. The tank sides were covered with mirrored tape to approximate infinite lateral boundary conditions, and the tank base was covered with a matt black plastic sheet to approximate no reflection from the ocean. A 3 mm opal polycarbonate sheet was placed 20 cm below the light rack to create a diffuse light field, and black curtains were draped between the lights and the tank to prevent reflection off the shiny cold room walls (Fig. <xref ref-type="fig" rid="Ch1.F1"/>). We deployed the photodiodes with 2.8 cm vertical resolution. The sensors were calibrated pre-deployment against the Metcon spectral radiometer. Before each experimental run, while sitting in open water, an additional correction was applied to the in situ sensor output to bring it in line with depth profiles measured using the spectral radiometer. We performed four experiments at growth temperatures of <inline-formula><mml:math id="M187" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">10</mml:mn></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M188" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">20</mml:mn></mml:mrow></mml:math></inline-formula> (two runs), and <inline-formula><mml:math id="M189" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">30</mml:mn></mml:mrow></mml:math></inline-formula> <inline-formula><mml:math id="M190" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C (Table <xref ref-type="table" rid="Ch1.T2"/>). For each run, we present vertical light profiles taken at the end of the growth phase, as well as <inline-formula><mml:math id="M191" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> calculated by two methods (Fig. <xref ref-type="fig" rid="Ch1.F10"/> and Table <xref ref-type="table" rid="Ch1.T3"/>). Method A <xref ref-type="bibr" rid="bib1.bibx8 bib1.bibx20" id="paren.53"/> uses
              <disp-formula id="Ch1.E8" content-type="numbered"><label>8</label><mml:math id="M192" display="block"><mml:mrow><mml:mi mathvariant="italic">κ</mml:mi><mml:mo>=</mml:mo><mml:mi>ln⁡</mml:mi><mml:mo mathsize="2.5em">(</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">1</mml:mn><mml:mo>-</mml:mo><mml:msub><mml:mi mathvariant="italic">ζ</mml:mi><mml:mi mathvariant="normal">s</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mstyle displaystyle="true"><mml:mfrac style="display"><mml:mrow><mml:mi>I</mml:mi><mml:mo>(</mml:mo><mml:mo>+</mml:mo><mml:mn mathvariant="normal">1.4</mml:mn><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mrow class="unit"><mml:mi mathvariant="normal">cm</mml:mi></mml:mrow><mml:mo>)</mml:mo></mml:mrow><mml:mrow><mml:mi>I</mml:mi><mml:mo>(</mml:mo><mml:mi>z</mml:mi><mml:mo>)</mml:mo></mml:mrow></mml:mfrac></mml:mstyle><mml:mo mathsize="2.5em">)</mml:mo><mml:mo>/</mml:mo><mml:mi>z</mml:mi><mml:mo>.</mml:mo></mml:mrow></mml:math></disp-formula>
            The depth, <inline-formula><mml:math id="M193" display="inline"><mml:mi>z</mml:mi></mml:math></inline-formula>, is chosen to be the shallowest sensor not yet frozen into the sea ice, and spectral reflectance, <inline-formula><mml:math id="M194" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">ζ</mml:mi><mml:mi mathvariant="normal">s</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, assumed to be 0.05. <xref ref-type="bibr" rid="bib1.bibx8" id="text.54"/> measured <inline-formula><mml:math id="M195" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> ranging from 3.1 to 4.7 m<inline-formula><mml:math id="M196" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> in 24 to 28 cm thick sea ice (Table <xref ref-type="table" rid="Ch1.T3"/>). <xref ref-type="bibr" rid="bib1.bibx20" id="text.55"/> measured <inline-formula><mml:math id="M197" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> ranging from 2.9 to 4.7 m<inline-formula><mml:math id="M198" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> in 17 to 27 cm thick sea ice. For method B, we also calculated <inline-formula><mml:math id="M199" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> by fitting a linear model to
              <disp-formula id="Ch1.E9" content-type="numbered"><label>9</label><mml:math id="M200" display="block"><mml:mrow><mml:mi>ln⁡</mml:mi><mml:mo>(</mml:mo><mml:mi>I</mml:mi><mml:mo>/</mml:mo><mml:mo>(</mml:mo><mml:msup><mml:mrow class="unit"><mml:mi mathvariant="normal">W</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:mi mathvariant="normal">m</mml:mi></mml:mrow><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup><mml:mo>)</mml:mo><mml:mo>)</mml:mo><mml:mo>=</mml:mo><mml:mo>-</mml:mo><mml:mi mathvariant="italic">κ</mml:mi><mml:mi>z</mml:mi><mml:mo>+</mml:mo><mml:mi>c</mml:mi><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            similar to <xref ref-type="bibr" rid="bib1.bibx27" id="paren.56"/>. Equation (<xref ref-type="disp-formula" rid="Ch1.E9"/>) represents a light field decaying exponentially with depth. While methods A and B both provide estimates of <inline-formula><mml:math id="M201" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula>, they do so in different ways, with method A using measurements external to the sea ice while method B uses only measurements within the sea ice. Previous studies, compared to our measurements in Table <xref ref-type="table" rid="Ch1.T3"/>, have calculated <inline-formula><mml:math id="M202" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> by iteratively solving the <xref ref-type="bibr" rid="bib1.bibx6" id="text.57"/> photometric model such that <inline-formula><mml:math id="M203" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> provides the best fit to measured albedo and transmission <xref ref-type="bibr" rid="bib1.bibx40" id="paren.58"/> – which we call method C.</p>

<table-wrap id="Ch1.T2" specific-use="star"><?xmltex \currentcnt{2}?><label>Table 2</label><caption><p id="d1e3886">Overview of experiments used to calculate with
PAR extinction coefficients. <inline-formula><mml:math id="M204" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">θ</mml:mi><mml:mi mathvariant="normal">atm</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> gives the mean atmospheric temperature during the experiment. <inline-formula><mml:math id="M205" display="inline"><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mi mathvariant="italic">ψ</mml:mi><mml:mrow><mml:msub><mml:mi mathvariant="italic">ψ</mml:mi><mml:mi mathvariant="normal">sat</mml:mi></mml:msub></mml:mrow></mml:mfrac></mml:mstyle></mml:math></inline-formula> is the mean relative humidity during the experiment, with <inline-formula><mml:math id="M206" display="inline"><mml:mi mathvariant="italic">ψ</mml:mi></mml:math></inline-formula> representing humidity and the subscript sat indicating saturation. <inline-formula><mml:math id="M207" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">f</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> gives the duration of the freeze up period. <inline-formula><mml:math id="M208" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> gives the maximum thickness attained, at which point <inline-formula><mml:math id="M209" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> was measured.</p></caption><oasis:table frame="topbot"><oasis:tgroup cols="6">
     <oasis:colspec colnum="1" colname="col1" align="left"/>
     <oasis:colspec colnum="2" colname="col2" align="right"/>
     <oasis:colspec colnum="3" colname="col3" align="center"/>
     <oasis:colspec colnum="4" colname="col4" align="right"/>
     <oasis:colspec colnum="5" colname="col5" align="center"/>
     <oasis:colspec colnum="6" colname="col6" align="left"/>
     <oasis:thead>
       <oasis:row>
         <oasis:entry colname="col1">Experiment</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M210" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">θ</mml:mi><mml:mi mathvariant="normal">atm</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / <inline-formula><mml:math id="M211" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C</oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M212" display="inline"><mml:mstyle displaystyle="false"><mml:mfrac style="text"><mml:mi mathvariant="italic">ψ</mml:mi><mml:mrow><mml:msub><mml:mi mathvariant="italic">ψ</mml:mi><mml:mi mathvariant="normal">sat</mml:mi></mml:msub></mml:mrow></mml:mfrac></mml:mstyle></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4"><inline-formula><mml:math id="M213" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">f</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / d</oasis:entry>
         <oasis:entry colname="col5"><inline-formula><mml:math id="M214" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / cm</oasis:entry>
         <oasis:entry colname="col6">Surface</oasis:entry>
       </oasis:row>
       <oasis:row rowsep="1">
         <oasis:entry colname="col1"/>
         <oasis:entry colname="col2"/>
         <oasis:entry colname="col3"/>
         <oasis:entry colname="col4"/>
         <oasis:entry colname="col5"/>
         <oasis:entry colname="col6">characteristics</oasis:entry>
       </oasis:row>
     </oasis:thead>
     <oasis:tbody>
       <oasis:row>
         <oasis:entry colname="col1">1</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M215" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">18.9</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.4</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M216" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.59</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.05</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4">6.1</oasis:entry>
         <oasis:entry colname="col5">18</oasis:entry>
         <oasis:entry colname="col6">Flooded</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">2</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M217" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">26.6</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M218" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.59</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.03</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4">5.1</oasis:entry>
         <oasis:entry colname="col5">18</oasis:entry>
         <oasis:entry colname="col6">Frost flowers</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">3</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M219" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">9.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M220" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.52</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.03</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4">10.1</oasis:entry>
         <oasis:entry colname="col5">15</oasis:entry>
         <oasis:entry colname="col6"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">4</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M221" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">18.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.5</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M222" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.60</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.05</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4">7.0</oasis:entry>
         <oasis:entry colname="col5">15</oasis:entry>
         <oasis:entry colname="col6"/>
       </oasis:row>
     </oasis:tbody>
   </oasis:tgroup></oasis:table></table-wrap>

<table-wrap id="Ch1.T3" specific-use="star"><?xmltex \currentcnt{3}?><label>Table 3</label><caption><p id="d1e4244">Comparison of PAR extinction coefficients (<inline-formula><mml:math id="M223" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula>, wavelength range 400 to 700 nm) produced by this work and by different studies.</p></caption><oasis:table frame="topbot"><oasis:tgroup cols="6">
     <oasis:colspec colnum="1" colname="col1" align="left"/>
     <oasis:colspec colnum="2" colname="col2" align="right"/>
     <oasis:colspec colnum="3" colname="col3" align="right"/>
     <oasis:colspec colnum="4" colname="col4" align="right"/>
     <oasis:colspec colnum="5" colname="col5" align="right"/>
     <oasis:colspec colnum="6" colname="col6" align="right"/>
     <oasis:thead>
       <oasis:row>
         <oasis:entry colname="col1">Experiment</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M230" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">θ</mml:mi><mml:mi mathvariant="normal">atm</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / <inline-formula><mml:math id="M231" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C</oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M232" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / cm</oasis:entry>
         <oasis:entry rowsep="1" namest="col4" nameend="col6" align="center"><inline-formula><mml:math id="M233" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> / m<inline-formula><mml:math id="M234" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula></oasis:entry>
       </oasis:row>
       <oasis:row rowsep="1">
         <oasis:entry colname="col1"/>
         <oasis:entry colname="col2"/>
         <oasis:entry colname="col3"/>
         <oasis:entry colname="col4">A</oasis:entry>
         <oasis:entry colname="col5">B</oasis:entry>
         <oasis:entry colname="col6">C</oasis:entry>
       </oasis:row>
     </oasis:thead>
     <oasis:tbody>
       <oasis:row>
         <oasis:entry colname="col1">This work, Exp. 1</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M235" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">18.9</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.4</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">18</oasis:entry>
         <oasis:entry colname="col4">5.3</oasis:entry>
         <oasis:entry colname="col5"><inline-formula><mml:math id="M236" display="inline"><mml:mrow><mml:mn mathvariant="normal">5.9</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">1.9</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col6"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">This work, Exp. 2</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M237" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">26.6</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">18</oasis:entry>
         <oasis:entry colname="col4">6.1</oasis:entry>
         <oasis:entry colname="col5"><inline-formula><mml:math id="M238" display="inline"><mml:mrow><mml:mn mathvariant="normal">4.4</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.7</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col6"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">This work, Exp. 3</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M239" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">9.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">15</oasis:entry>
         <oasis:entry colname="col4">4.1</oasis:entry>
         <oasis:entry colname="col5"><inline-formula><mml:math id="M240" display="inline"><mml:mrow><mml:mn mathvariant="normal">4.5</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">1.9</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col6"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">This work, Exp. 4</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M241" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">18.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.5</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">15</oasis:entry>
         <oasis:entry colname="col4">3.7</oasis:entry>
         <oasis:entry colname="col5"><inline-formula><mml:math id="M242" display="inline"><mml:mrow><mml:mn mathvariant="normal">6.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">1.7</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col6"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Nice<inline-formula><mml:math id="M243" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">1</mml:mn></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M244" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">10</mml:mn></mml:mrow></mml:math></inline-formula> to <inline-formula><mml:math id="M245" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">20</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">17 to 27</oasis:entry>
         <oasis:entry colname="col4">2.9 to 4.7</oasis:entry>
         <oasis:entry colname="col5"/>
         <oasis:entry colname="col6"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Santala Bay, Finland<inline-formula><mml:math id="M246" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M247" display="inline"><mml:mrow><mml:mo>&lt;</mml:mo><mml:mo>-</mml:mo><mml:mn mathvariant="normal">5</mml:mn></mml:mrow></mml:math></inline-formula> to 5</oasis:entry>
         <oasis:entry colname="col3">24 to 28</oasis:entry>
         <oasis:entry colname="col4">3.1 to 4.7</oasis:entry>
         <oasis:entry colname="col5"/>
         <oasis:entry colname="col6"/>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Laboratory<inline-formula><mml:math id="M248" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M249" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">10</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">28</oasis:entry>
         <oasis:entry colname="col4"/>
         <oasis:entry colname="col5"/>
         <oasis:entry colname="col6">2.5</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Laboratory<inline-formula><mml:math id="M250" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M251" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">30</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">28</oasis:entry>
         <oasis:entry colname="col4"/>
         <oasis:entry colname="col5"/>
         <oasis:entry colname="col6">3</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">Laboratory<inline-formula><mml:math id="M252" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M253" display="inline"><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">15</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col3">40</oasis:entry>
         <oasis:entry colname="col4"/>
         <oasis:entry colname="col5">3 to 10</oasis:entry>
         <oasis:entry colname="col6"/>
       </oasis:row>
     </oasis:tbody>
   </oasis:tgroup></oasis:table><table-wrap-foot><p id="d1e4254"><inline-formula><mml:math id="M224" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">1</mml:mn></mml:msup></mml:math></inline-formula> <xref ref-type="bibr" rid="bib1.bibx20" id="text.59"/>;  <inline-formula><mml:math id="M225" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:math></inline-formula> <xref ref-type="bibr" rid="bib1.bibx8" id="text.60"/>;  <inline-formula><mml:math id="M226" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">3</mml:mn></mml:msup></mml:math></inline-formula> <xref ref-type="bibr" rid="bib1.bibx40" id="text.61"/>;  <inline-formula><mml:math id="M227" display="inline"><mml:msup><mml:mi/><mml:mn mathvariant="normal">4</mml:mn></mml:msup></mml:math></inline-formula> <xref ref-type="bibr" rid="bib1.bibx27" id="text.62"/>. <?xmltex \hack{\\}?>A – Eq. <xref ref-type="disp-formula" rid="Ch1.E8"/>.
B – Eq. <xref ref-type="disp-formula" rid="Ch1.E9"/>. Values from this work are broadband <inline-formula><mml:math id="M228" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula>, while the range of values given for <xref ref-type="bibr" rid="bib1.bibx27" id="text.63"/> cover a range of <inline-formula><mml:math id="M229" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> at wavelengths between 350 and 650 nm.
C – <xref ref-type="bibr" rid="bib1.bibx6" id="text.64"/> model iteratively solved.</p></table-wrap-foot></table-wrap>

      <?xmltex \floatpos{t}?><fig id="Ch1.F10"><?xmltex \currentcnt{10}?><?xmltex \def\figurename{Figure}?><label>Figure 10</label><caption><p id="d1e4804">Irradiance profiles <bold>(a)</bold> and linear regressions of <inline-formula><mml:math id="M254" display="inline"><mml:mrow><mml:mi>ln⁡</mml:mi><mml:mo>(</mml:mo><mml:mi>I</mml:mi><mml:mo>/</mml:mo><mml:mrow class="unit"><mml:mi mathvariant="normal">W</mml:mi><mml:mspace width="0.125em" linebreak="nobreak"/><mml:msup><mml:mi mathvariant="normal">m</mml:mi><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:mrow></mml:math></inline-formula>) vs. <inline-formula><mml:math id="M255" display="inline"><mml:mi>z</mml:mi></mml:math></inline-formula> <bold>(b)</bold> used to produce extinction coefficients (Eqs. <xref ref-type="disp-formula" rid="Ch1.E8"/> and <xref ref-type="disp-formula" rid="Ch1.E9"/>) from the final profile of the freezing period. The legend in <bold>(b)</bold> gives the extinction coefficient for a given profile with 1 standard error.</p></caption>
            <?xmltex \igopts{width=199.169291pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f10.png"/>

          </fig>

      <p id="d1e4860">Measured light profiles taken at the end of four experiments are shown in Fig. <xref ref-type="fig" rid="Ch1.F10"/>. The thickness varied by up to 3 cm between the four experiments. At the end of the experiments, runs 1 and 2 had six sensors frozen into the sea ice and experiments 3 and 4 had five sensors frozen into the sea ice. The light intensity at 1.4 cm above the sea ice was between 7.7 and 8.9 W m<inline-formula><mml:math id="M256" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> and reduced to between 2.8 and 3.6 W m<inline-formula><mml:math id="M257" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> at 18.2 cm depth. For experiments 1 and 4, the shallowest frozen sensor measured higher intensity than the sensor above the sea ice, which is a phenomenon that was predicted by <xref ref-type="bibr" rid="bib1.bibx19" id="text.65"/> and that is due to the change in refractive index across the air–ice interface.</p>
      <?pagebreak page1843?><p id="d1e4892">Using method A, our calculated <inline-formula><mml:math id="M258" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> ranges from 3.7 (experiment 4) to 6.1 m<inline-formula><mml:math id="M259" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (experiment 2). Using method B, our calculated <inline-formula><mml:math id="M260" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> ranges from 4.4 (experiment 2) to 6.2 m<inline-formula><mml:math id="M261" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (experiment 4). For both methods, the range of <inline-formula><mml:math id="M262" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> observed in our tank overlaps with the range of <inline-formula><mml:math id="M263" display="inline"><mml:mi mathvariant="italic">κ</mml:mi></mml:math></inline-formula> observed previously for thin sea ice. These results build confidence that the RvG-ASIC is a useful tool for future biological or photochemical experiments in young sea ice and validate this particular experimental setup for experiments involving visible light.</p>
</sec>
<sec id="Ch1.S3.SS2.SSS2">
  <label>3.2.2</label><title>Estimating sea-ice bulk salinity</title>
      <p id="d1e4956">Bulk salinity is a sea-ice state variable that, when measured alongside temperature, can allow for estimation of the sea-ice liquid fraction. Growing sea ice desalinates rapidly by gravity drainage <xref ref-type="bibr" rid="bib1.bibx37" id="paren.66"/>. The bulk salinity of natural, young sea ice has been measured using cores <xref ref-type="bibr" rid="bib1.bibx56" id="paren.67"/> and wireharps <xref ref-type="bibr" rid="bib1.bibx37" id="paren.68"/>. <xref ref-type="bibr" rid="bib1.bibx56" id="text.69"/> sampled young sea ice in North Star Bay, Greenland, in growing sea ice between 5 and 23 cm thick. Salinities ranged from around 5 to 20 g kg<inline-formula><mml:math id="M264" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> vertically with mean salinities decreasing from 20 g kg<inline-formula><mml:math id="M265" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> early in the growth to around 10 g kg<inline-formula><mml:math id="M266" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> after 10 d. <xref ref-type="bibr" rid="bib1.bibx37" id="text.70"/> cut a hole in Arctic sea ice and measured the salinity profile, in situ, as it refroze up to 17 cm thickness (the depth range of their instrument). Over two experiments, they measured bulk salinities ranging from around 35 g kg<inline-formula><mml:math id="M267" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> at the sea-ice–ocean interface to around 4 g kg<inline-formula><mml:math id="M268" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> in the interior. Mean salinities decreased from around 35 towards 10 g kg<inline-formula><mml:math id="M269" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (experiment 1) and around 15 g kg<inline-formula><mml:math id="M270" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (experiment 2). Here, we present bulk salinities for sea ice grown in the RvG-ASIC, comparing it to the range of salinities observed in nature.
<?xmltex \hack{\break}?>Quantifying the vertical bulk salinity profile in sea-ice cores – the most common sea-ice sampling methodology – is difficult due to the known bias during coring of brine loss and bulk salinity underestimation. Several other methodologies have been proposed. <xref ref-type="bibr" rid="bib1.bibx2" id="text.71"/> present a destructive sampling methodology that attempts to prevent brine loss upon sampling (Sect. <xref ref-type="sec" rid="Ch1.S2.SS3.SSS3"/>). <xref ref-type="bibr" rid="bib1.bibx39" id="text.72"/> present instrumentation, called a wireharp, to quantify the in situ bulk salinity profile. Several groups have presented gravity drainage parameterisations that quantify the bulk salinity profile during sea-ice growth <xref ref-type="bibr" rid="bib1.bibx14 bib1.bibx42 bib1.bibx4 bib1.bibx52 bib1.bibx51 bib1.bibx18" id="paren.73"/>, noting that gravity drainage is the dominant process redistributing salt in growing sea ice <xref ref-type="bibr" rid="bib1.bibx38" id="paren.74"/>.</p>
      <p id="d1e5077">We performed two sea-ice growth experiments in the RvG-ASIC and estimated the sea-ice salinity by (1) constructing a<?pagebreak page1844?> salt and mass budget, (2) taking sea-ice cores, (3) taking sea-ice slabs <xref ref-type="bibr" rid="bib1.bibx2" id="paren.75"/>, (4) using a wireharp <xref ref-type="bibr" rid="bib1.bibx39" id="paren.76"/>, and (5) using the <xref ref-type="bibr" rid="bib1.bibx14" id="text.77"/> gravity drainage parameterisation. Our artificial ocean was composed of Tropic Marin sea salt and deionised water such that <inline-formula><mml:math id="M271" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">31.3</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> g kg<inline-formula><mml:math id="M272" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>. We grew free-floating sea ice to <inline-formula><mml:math id="M273" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mn mathvariant="normal">11.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> cm (run 1) and (16.4 <inline-formula><mml:math id="M274" display="inline"><mml:mo>±</mml:mo></mml:math></inline-formula> 2.7) cm (run 2) thickness (mean and standard deviation of measured ice core thickness). Measurements of sea-ice temperature, thickness, and the initial ocean salinity were used to force the model, which has been used previously to model experiments in the RvG-ASIC <xref ref-type="bibr" rid="bib1.bibx11 bib1.bibx49" id="paren.78"/>. We used the <xref ref-type="bibr" rid="bib1.bibx14" id="text.79"/> gravity drainage parameterisation, because it performed well in previous studies <xref ref-type="bibr" rid="bib1.bibx49" id="paren.80"/> and has tuning parameters shown to perform well for Arctic field data <xref ref-type="bibr" rid="bib1.bibx15" id="paren.81"/>. The model has two tuning parameters: the critical Rayleigh number, <inline-formula><mml:math id="M275" display="inline"><mml:mrow><mml:mi>R</mml:mi><mml:msub><mml:mi>a</mml:mi><mml:mi mathvariant="normal">c</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, and the desalination strength, <inline-formula><mml:math id="M276" display="inline"><mml:mi mathvariant="italic">α</mml:mi></mml:math></inline-formula>. We estimate an uncertainty on the modelling by running the model for each of the three sets of tuning parameters presented in <xref ref-type="bibr" rid="bib1.bibx15" id="text.82"/> (Table <xref ref-type="table" rid="Ch1.T4"/>). The model is forced by measured temperature profiles and sea-ice thickness in lieu of modelled thermodynamics (Fig. <xref ref-type="fig" rid="Ch1.F11"/>). Cores and slabs were extracted according to Sect. <xref ref-type="sec" rid="Ch1.S2.SS3.SSS3"/> and sectioned vertically into 1 to 2 cm layers. Ice samples were melted and placed in a 20 <inline-formula><mml:math id="M277" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C thermostatic bath. Conductivity was measured using a conductivity probe (Orion) and calibrated with certified reference material (Thermo Scientific Eutech Handheld Meters Calibration Solution). Bulk salinity was derived using the GSW toolbox <xref ref-type="bibr" rid="bib1.bibx29" id="paren.83"/>. We discarded three discrete samples (one from the cores and two from the slabs for run 1) because of punctured sample bags. A single wireharp profile was taken in the middle of the tank and used to derive <inline-formula><mml:math id="M278" display="inline"><mml:mi mathvariant="italic">ϕ</mml:mi></mml:math></inline-formula> and <inline-formula><mml:math id="M279" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> (Eqs. <xref ref-type="disp-formula" rid="Ch1.E1"/> and <xref ref-type="disp-formula" rid="Ch1.E2"/>).</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F11"><?xmltex \currentcnt{11}?><?xmltex \def\figurename{Figure}?><label>Figure 11</label><caption><p id="d1e5227">Vertical temperature (<bold>a</bold> and <bold>c</bold>) and bulk salinity profiles (<bold>b</bold> and <bold>d</bold>) for two experimental runs. In <bold>(a)</bold> and <bold>(c)</bold> the model temperature shows the model forcing, which was produced using measured temperature profiles; panels <bold>(b)</bold> and <bold>(d)</bold> show salinity estimated from cores, slabs <xref ref-type="bibr" rid="bib1.bibx2" id="paren.84"/>, wireharps <xref ref-type="bibr" rid="bib1.bibx39" id="paren.85"/>, and a gravity drainage model. The horizontal box length shows the median 1 standard deviation from repeat measurements at a given depth (cores and slabs), and the vertical box length shows the depth covered by the sample layer. Wireharp errors were calculated using methodology presented in <xref ref-type="bibr" rid="bib1.bibx60" id="text.86"/>. The model used the <xref ref-type="bibr" rid="bib1.bibx14" id="text.87"/> gravity drainage parameterisation with tuning parameters taken from <xref ref-type="bibr" rid="bib1.bibx15" id="text.88"/>. The solid line represents their best estimate tuning parameter set. The dotted bounds on the model show output using two other plausible sets of tuning parameters presented in <xref ref-type="bibr" rid="bib1.bibx15" id="text.89"/>.</p></caption>
            <?xmltex \igopts{width=236.157874pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f11.png"/>

          </fig>

      <?pagebreak page1845?><p id="d1e5281">The mass balance was constructed by conservation of mass and salt from the start of each run (<inline-formula><mml:math id="M280" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula>) to the final sampling (<inline-formula><mml:math id="M281" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">end</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>). The conservation of mass gives
              <disp-formula id="Ch1.E10" content-type="numbered"><label>10</label><mml:math id="M282" display="block"><mml:mrow><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">sys</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>+</mml:mo><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where <inline-formula><mml:math id="M283" display="inline"><mml:mi>m</mml:mi></mml:math></inline-formula> is mass, and the subscripts sys, o, and si indicate the experimental system, ocean, and sea-ice compartments, respectively. Noting that <inline-formula><mml:math id="M284" display="inline"><mml:mrow><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">sys</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula>, the conservation of salt at the end of the experiment (<inline-formula><mml:math id="M285" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">end</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>) gives
              <disp-formula id="Ch1.E11" content-type="numbered"><label>11</label><mml:math id="M286" display="block"><mml:mrow><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">sys</mml:mi></mml:msub><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:mo>)</mml:mo><mml:mo>=</mml:mo><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">end</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mo>+</mml:mo><mml:msub><mml:mi>m</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>.</mml:mo></mml:mrow></mml:math></disp-formula>
            Our desired variable is the vertically integrated bulk sea-ice salinity, <inline-formula><mml:math id="M287" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, which we recover by substituting Eq. (<xref ref-type="disp-formula" rid="Ch1.E10"/>) into Eq. (<xref ref-type="disp-formula" rid="Ch1.E11"/>), giving
              <disp-formula id="Ch1.E12" content-type="numbered"><label>12</label><mml:math id="M288" display="block"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mi>r</mml:mi><mml:mi mathvariant="normal">Δ</mml:mi><mml:mi>S</mml:mi><mml:mo>+</mml:mo><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">end</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where
              <disp-formula id="Ch1.E13" content-type="numbered"><label>13</label><mml:math id="M289" display="block"><mml:mrow><mml:mi mathvariant="normal">Δ</mml:mi><mml:mi>S</mml:mi><mml:mo>=</mml:mo><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:mo>)</mml:mo><mml:mo>-</mml:mo><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">end</mml:mi></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:math></disp-formula>
            and <inline-formula><mml:math id="M290" display="inline"><mml:mi>r</mml:mi></mml:math></inline-formula> gives the ratio of the mass of the experimental system to the mass of the sea ice at final sampling, such that
              <disp-formula id="Ch1.E14" content-type="numbered"><label>14</label><mml:math id="M291" display="block"><mml:mrow><mml:mi>r</mml:mi><mml:mo>=</mml:mo><mml:mstyle displaystyle="true"><mml:mfrac style="display"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:mo>)</mml:mo><mml:msub><mml:mi mathvariant="italic">ρ</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:mo>)</mml:mo></mml:mrow><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:msub><mml:mi mathvariant="italic">ρ</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:mfrac></mml:mstyle><mml:mo>.</mml:mo></mml:mrow></mml:math></disp-formula>
            The error on Eq. (<xref ref-type="disp-formula" rid="Ch1.E12"/>) was calculated by Gaussian propagation, such that
              <disp-formula id="Ch1.E15" content-type="numbered"><label>15</label><mml:math id="M292" display="block"><mml:mrow><?xmltex \hack{\hbox\bgroup\fontsize{9.0}{9.0}\selectfont$\displaystyle}?><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mo>=</mml:mo><mml:msqrt><mml:mrow><mml:msup><mml:mi>r</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msup><mml:mi mathvariant="normal">Δ</mml:mi><mml:msup><mml:mi>S</mml:mi><mml:mn mathvariant="normal">2</mml:mn></mml:msup><mml:mo mathsize="2.5em">(</mml:mo><mml:mo mathsize="2.0em">(</mml:mo><mml:mstyle displaystyle="true"><mml:mfrac style="display"><mml:mrow><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:mi>r</mml:mi><mml:mo>)</mml:mo></mml:mrow><mml:mi>r</mml:mi></mml:mfrac></mml:mstyle><mml:msup><mml:mo mathsize="2.0em">)</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:msup><mml:mo>+</mml:mo><mml:mo mathsize="2.0em">(</mml:mo><mml:mstyle displaystyle="true"><mml:mfrac style="display"><mml:mrow><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:mi mathvariant="normal">Δ</mml:mi><mml:mi>S</mml:mi><mml:mo>)</mml:mo></mml:mrow><mml:mrow><mml:mi mathvariant="normal">Δ</mml:mi><mml:mi>S</mml:mi></mml:mrow></mml:mfrac></mml:mstyle><mml:msup><mml:mo mathsize="2.0em">)</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:msup><mml:mo mathsize="2.5em">)</mml:mo><mml:mo>+</mml:mo><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:msup><mml:mo>)</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:msup></mml:mrow></mml:msqrt><mml:mo>,</mml:mo><?xmltex \hack{$\egroup}?></mml:mrow></mml:math></disp-formula>
            where <inline-formula><mml:math id="M293" display="inline"><mml:mi>u</mml:mi></mml:math></inline-formula> gives the uncertainty of the individual terms. The dominant uncertainty, accounting for <inline-formula><mml:math id="M294" display="inline"><mml:mrow><mml:mo>&gt;</mml:mo><mml:mn mathvariant="normal">95</mml:mn></mml:mrow></mml:math></inline-formula> % of <inline-formula><mml:math id="M295" display="inline"><mml:mrow><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula>, propagates from <inline-formula><mml:math id="M296" display="inline"><mml:mrow><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:mi>r</mml:mi><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> and derives chiefly from the variability in measured <inline-formula><mml:math id="M297" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. The errors on <inline-formula><mml:math id="M298" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M299" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub><mml:mo>(</mml:mo><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">end</mml:mi></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> are highly correlated given the stability of the salinity sensor over the short duration of the experiment, and as such <inline-formula><mml:math id="M300" display="inline"><mml:mrow><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:mi mathvariant="normal">Δ</mml:mi><mml:mi>S</mml:mi><mml:mo>)</mml:mo><mml:mo>≪</mml:mo><mml:mn mathvariant="normal">0.01</mml:mn></mml:mrow></mml:math></inline-formula> g kg<inline-formula><mml:math id="M301" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> and has a negligible impact on <inline-formula><mml:math id="M302" display="inline"><mml:mrow><mml:mi>u</mml:mi><mml:mo>(</mml:mo><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula>. The sensitivity of <inline-formula><mml:math id="M303" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> to our choice of <inline-formula><mml:math id="M304" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">ρ</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is around 0.02 (g kg<inline-formula><mml:math id="M305" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>) / (kg m<inline-formula><mml:math id="M306" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>), such that a 20 kg m<inline-formula><mml:math id="M307" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> increase in <inline-formula><mml:math id="M308" display="inline"><mml:mrow><mml:msub><mml:mi mathvariant="italic">ρ</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> causes a 0.4 g kg<inline-formula><mml:math id="M309" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> increase in our estimated <inline-formula><mml:math id="M310" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, which is well within our uncertainty bounds.</p>
      <p id="d1e5971">We first turn to the vertical profiles of <inline-formula><mml:math id="M311" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> (Fig. <xref ref-type="fig" rid="Ch1.F11"/>). For both runs, the wireharp profile at low frequency including the electrical double-layer correction proposed by <xref ref-type="bibr" rid="bib1.bibx39" id="text.90"/> is very similar to the high-frequency wireharp profile without such correction. This confirms that at higher frequencies the impact of the double layer becomes less important, and no more correction to the profile is necessary. For simplicity, and because the high-frequency channel has not yet registered ice growth in the deepest run 1 sensor, we proceed by discussing only the low-frequency channel. For both runs, and for all profiles, there is a salinity maximum near the sea-ice–ocean interface (<inline-formula><mml:math id="M312" display="inline"><mml:mrow><mml:mi>z</mml:mi><mml:mo>=</mml:mo><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>). The model and the wireharps converge towards <inline-formula><mml:math id="M313" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> as <inline-formula><mml:math id="M314" display="inline"><mml:mi>z</mml:mi></mml:math></inline-formula> approaches <inline-formula><mml:math id="M315" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. The cores and the slabs produce the lowest <inline-formula><mml:math id="M316" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> near this lower interface, with the slabs giving higher <inline-formula><mml:math id="M317" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> relative to the cores in this region in run 2. For run 1, the wireharps generally measure the highest <inline-formula><mml:math id="M318" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, the cores and slabs are similar and measure the lowest <inline-formula><mml:math id="M319" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, and the model is intermediate. For run 2, the cores, slabs, wireharps, and model are generally consistent between <inline-formula><mml:math id="M320" display="inline"><mml:mrow><mml:mi>z</mml:mi><mml:mo>/</mml:mo><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>=</mml:mo></mml:mrow></mml:math></inline-formula> 0.6 to 0.1. Near the upper interface, the model predicts higher <inline-formula><mml:math id="M321" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> than the measurements, and the wireharps give the lowest <inline-formula><mml:math id="M322" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. For run 1, the cores and slabs are consistent throughout, but the wireharps, model, and discrete samples generally disagree. Towards the sea-ice–atmosphere interface (<inline-formula><mml:math id="M323" display="inline"><mml:mrow><mml:mi>z</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">0</mml:mn></mml:mrow></mml:math></inline-formula>), the wireharps produce minima in <inline-formula><mml:math id="M324" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. The cores and slabs show an <inline-formula><mml:math id="M325" display="inline"><mml:mrow><mml:mo>≈</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:math></inline-formula> g kg<inline-formula><mml:math id="M326" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> increase in <inline-formula><mml:math id="M327" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> for the shallowest layer relative to the layer below, forming a C-shaped profile. Modelled <inline-formula><mml:math id="M328" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> at <inline-formula><mml:math id="M329" display="inline"><mml:mrow><mml:mi>z</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">0</mml:mn></mml:mrow></mml:math></inline-formula> is around 10 g kg<inline-formula><mml:math id="M330" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> greater than in the interior, forming a C-shaped profile that is more pronounced than in the discrete samples at both interfaces. The difference in <inline-formula><mml:math id="M331" display="inline"><mml:mi mathvariant="italic">θ</mml:mi></mml:math></inline-formula> between the wireharps and the forcing is at most 0.2 <inline-formula><mml:math id="M332" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C which, depending on <inline-formula><mml:math id="M333" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, translates to a 2 % to 3 % difference in <inline-formula><mml:math id="M334" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>.</p>
      <p id="d1e6250">The mass balance produces <inline-formula><mml:math id="M335" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">11.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">2.0</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> g kg<inline-formula><mml:math id="M336" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (run 1) and <inline-formula><mml:math id="M337" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mo>(</mml:mo><mml:mn mathvariant="normal">11.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">4.1</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> g kg<inline-formula><mml:math id="M338" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (run 2) (mean and standard deviation, Table <xref ref-type="table" rid="Ch1.T4"/>). These values represent the vertically integrated bulk sea-ice salinity, averaged over the tank footprint. We compare the measurements and model to the mass balance by calculating <inline-formula><mml:math id="M339" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> for the profile produced by each method at the end of each run. For the cores and slabs, we first averaged the individual layer measurements for each sample and then took the average of these samples to be <inline-formula><mml:math id="M340" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. For the wireharps, we linearly interpolated measured <inline-formula><mml:math id="M341" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> to the midpoint between each measurement, and took the mean of these interpolated <inline-formula><mml:math id="M342" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> to produce <inline-formula><mml:math id="M343" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. In this way, we more closely approximate the treatment of the cores and slabs, for which the edge of the bottommost layer is at <inline-formula><mml:math id="M344" display="inline"><mml:mrow><mml:mi>z</mml:mi><mml:mo>=</mml:mo><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. Model <inline-formula><mml:math id="M345" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is the mean of the <inline-formula><mml:math id="M346" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> of individual model layers. The cores and slabs perform similarly, underestimating the mass balance for each run. The wireharps overestimate the mass balance in run 1 and capture the run 2 mass balance to within 1 standard deviation. The best estimate model <inline-formula><mml:math id="M347" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is consistent with the mass balance for both runs.</p>
      <p id="d1e6451">The underestimation of <inline-formula><mml:math id="M348" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> by the cores (Table <xref ref-type="table" rid="Ch1.T4"/>) highlights a known bias in sea-ice core bulk salinity measurement, which is that of brine loss upon sampling. This bias is apparent in the vertical profiles (Fig. <xref ref-type="fig" rid="Ch1.F11"/>), where cores give the lowest estimates of <inline-formula><mml:math id="M349" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> in the lower third of the profile, which we expect to be most affected by brine loss. The slabs perform similarly to the cores, potentially due to brine drainage during the shock freezing process. The slabs retain<?pagebreak page1846?> more brine than the cores in run 2, as shown by their higher <inline-formula><mml:math id="M350" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> in the lower portion and their higher <inline-formula><mml:math id="M351" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. Previous work has tuned gravity drainage parameterisations to slab <inline-formula><mml:math id="M352" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> measurements <xref ref-type="bibr" rid="bib1.bibx49" id="paren.91"/>, and these tuning parameters may therefore be biased to predict too much desalination. The wireharp performance is more variable, overestimating <inline-formula><mml:math id="M353" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> in run 1 and capturing the mass balance in run 2. The performance of the model, forced with measured <inline-formula><mml:math id="M354" display="inline"><mml:mi mathvariant="italic">θ</mml:mi></mml:math></inline-formula> and <inline-formula><mml:math id="M355" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, solely reflects the gravity drainage parameterisation and its tuning <xref ref-type="bibr" rid="bib1.bibx14 bib1.bibx15" id="paren.92"/>, incorporating minimal error from thermodynamics. Only the model captures the mass balance for both runs. The mass balance is, for both runs, within the range of young sea-ice salinities observed in nature <xref ref-type="bibr" rid="bib1.bibx56 bib1.bibx37" id="paren.93"/>.</p>

<table-wrap id="Ch1.T4" specific-use="star"><?xmltex \currentcnt{4}?><label>Table 4</label><caption><p id="d1e6564">Results for the mean bulk salinity, <inline-formula><mml:math id="M356" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, calculated from five methodologies for two experimental runs. <inline-formula><mml:math id="M357" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">f</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is the duration of the freezing period, and <inline-formula><mml:math id="M358" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is the mean sea-ice thickness as measured in cores at the end of each run. The number of samples or profiles is given in brackets. With the exception of the model, all uncertainties are 1 standard deviation. Mass balance, core, and slab uncertainties were calculated based on repeat measurements. Wireharp uncertainties were calculated by propagation of the uncertainties on <inline-formula><mml:math id="M359" display="inline"><mml:mrow><mml:msub><mml:mi>R</mml:mi><mml:mn mathvariant="normal">0</mml:mn></mml:msub></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M360" display="inline"><mml:mi mathvariant="italic">θ</mml:mi></mml:math></inline-formula> for individual wire pairs. The model gives <inline-formula><mml:math id="M361" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> as predicted using the three parameter sets presented in <xref ref-type="bibr" rid="bib1.bibx15" id="text.94"/>. Low bound: <inline-formula><mml:math id="M362" display="inline"><mml:mrow><mml:mi>R</mml:mi><mml:msub><mml:mi>a</mml:mi><mml:mi mathvariant="normal">c</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mn mathvariant="normal">3.23</mml:mn></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M363" display="inline"><mml:mrow><mml:mi mathvariant="italic">α</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">0.000681</mml:mn></mml:mrow></mml:math></inline-formula> kg m<inline-formula><mml:math id="M364" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> s<inline-formula><mml:math id="M365" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>. Best estimate: <inline-formula><mml:math id="M366" display="inline"><mml:mrow><mml:mi>R</mml:mi><mml:msub><mml:mi>a</mml:mi><mml:mi mathvariant="normal">c</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mn mathvariant="normal">4.89</mml:mn></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M367" display="inline"><mml:mrow><mml:mi mathvariant="italic">α</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">0.000584</mml:mn></mml:mrow></mml:math></inline-formula> kg m<inline-formula><mml:math id="M368" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> s<inline-formula><mml:math id="M369" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>. High bound: <inline-formula><mml:math id="M370" display="inline"><mml:mrow><mml:mi>R</mml:mi><mml:msub><mml:mi>a</mml:mi><mml:mi mathvariant="normal">c</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mn mathvariant="normal">7.10</mml:mn></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M371" display="inline"><mml:mrow><mml:mi mathvariant="italic">α</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">0.000510</mml:mn></mml:mrow></mml:math></inline-formula> kg m<inline-formula><mml:math id="M372" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">3</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> s<inline-formula><mml:math id="M373" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>.</p></caption><oasis:table frame="topbot"><oasis:tgroup cols="10">
     <oasis:colspec colnum="1" colname="col1" align="left"/>
     <oasis:colspec colnum="2" colname="col2" align="center"/>
     <oasis:colspec colnum="3" colname="col3" align="center"/>
     <oasis:colspec colnum="4" colname="col4" align="center"/>
     <oasis:colspec colnum="5" colname="col5" align="center" colsep="1"/>
     <oasis:colspec colnum="6" colname="col6" align="center"/>
     <oasis:colspec colnum="7" colname="col7" align="center"/>
     <oasis:colspec colnum="8" colname="col8" align="center"/>
     <oasis:colspec colnum="9" colname="col9" align="center"/>
     <oasis:colspec colnum="10" colname="col10" align="center"/>
     <oasis:thead>
       <oasis:row>
         <oasis:entry colname="col1">Run</oasis:entry>
         <oasis:entry colname="col2"><inline-formula><mml:math id="M379" display="inline"><mml:mrow><mml:msub><mml:mi>t</mml:mi><mml:mi mathvariant="normal">f</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / d</oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M380" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / cm</oasis:entry>
         <oasis:entry rowsep="1" namest="col4" nameend="col5" colsep="1"><inline-formula><mml:math id="M381" display="inline"><mml:mrow><mml:msub><mml:mi>S</mml:mi><mml:mi mathvariant="normal">o</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / (g kg<inline-formula><mml:math id="M382" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>)<inline-formula><mml:math id="M383" display="inline"><mml:msup><mml:mi/><mml:mi mathvariant="normal">a</mml:mi></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry rowsep="1" namest="col6" nameend="col10"><inline-formula><mml:math id="M384" display="inline"><mml:mrow><mml:msub><mml:mover accent="true"><mml:mi>S</mml:mi><mml:mo mathvariant="normal">‾</mml:mo></mml:mover><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> / (g kg<inline-formula><mml:math id="M385" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>) </oasis:entry>
       </oasis:row>
       <oasis:row rowsep="1">
         <oasis:entry colname="col1"/>
         <oasis:entry colname="col2"/>
         <oasis:entry colname="col3"/>
         <oasis:entry colname="col4">Start</oasis:entry>
         <oasis:entry colname="col5">End</oasis:entry>
         <oasis:entry colname="col6">Mass balance</oasis:entry>
         <oasis:entry colname="col7">Cores</oasis:entry>
         <oasis:entry colname="col8">Slabs</oasis:entry>
         <oasis:entry colname="col9">Wireharp<inline-formula><mml:math id="M386" display="inline"><mml:msup><mml:mi/><mml:mi mathvariant="normal">b</mml:mi></mml:msup></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col10">Model</oasis:entry>
       </oasis:row>
     </oasis:thead>
     <oasis:tbody>
       <oasis:row>
         <oasis:entry colname="col1">1</oasis:entry>
         <oasis:entry colname="col2">3.6</oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M387" display="inline"><mml:mrow><mml:mn mathvariant="normal">11.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.9</mml:mn><mml:mspace width="0.25em" linebreak="nobreak"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">10</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4">31.3</oasis:entry>
         <oasis:entry colname="col5">33.9</oasis:entry>
         <oasis:entry colname="col6"><inline-formula><mml:math id="M388" display="inline"><mml:mrow><mml:mn mathvariant="normal">11.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">2.0</mml:mn><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">10</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col7"><inline-formula><mml:math id="M389" display="inline"><mml:mrow><mml:mn mathvariant="normal">8.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.2</mml:mn><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">3</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col8"><inline-formula><mml:math id="M390" display="inline"><mml:mrow><mml:mn mathvariant="normal">8.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.5</mml:mn><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">2</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col9"><inline-formula><mml:math id="M391" display="inline"><mml:mrow><mml:mn mathvariant="normal">15.8</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.5</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col10">12.9 [12.0 to 13.7]</oasis:entry>
       </oasis:row>
       <oasis:row>
         <oasis:entry colname="col1">2</oasis:entry>
         <oasis:entry colname="col2">6.5</oasis:entry>
         <oasis:entry colname="col3"><inline-formula><mml:math id="M392" display="inline"><mml:mrow><mml:mn mathvariant="normal">16.4</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">2.7</mml:mn><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">10</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col4">31.3</oasis:entry>
         <oasis:entry colname="col5">35.4</oasis:entry>
         <oasis:entry colname="col6"><inline-formula><mml:math id="M393" display="inline"><mml:mrow><mml:mn mathvariant="normal">11.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">4.1</mml:mn><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">10</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col7"><inline-formula><mml:math id="M394" display="inline"><mml:mrow><mml:mn mathvariant="normal">6.5</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.5</mml:mn><mml:mspace width="0.25em" linebreak="nobreak"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">3</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col8"><inline-formula><mml:math id="M395" display="inline"><mml:mrow><mml:mn mathvariant="normal">7.3</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.2</mml:mn><mml:mspace linebreak="nobreak" width="0.25em"/><mml:mo>(</mml:mo><mml:mn mathvariant="normal">3</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col9"><inline-formula><mml:math id="M396" display="inline"><mml:mrow><mml:mn mathvariant="normal">10.0</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.4</mml:mn></mml:mrow></mml:math></inline-formula></oasis:entry>
         <oasis:entry colname="col10">11.6 [10.8 to 12.5]</oasis:entry>
       </oasis:row>
     </oasis:tbody>
   </oasis:tgroup></oasis:table><table-wrap-foot><p id="d1e6800"><inline-formula><mml:math id="M374" display="inline"><mml:msup><mml:mi/><mml:mi mathvariant="normal">a</mml:mi></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M375" display="inline"><mml:mrow><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn></mml:mrow></mml:math></inline-formula> g kg<inline-formula><mml:math id="M376" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>. <?xmltex \hack{\\}?><inline-formula><mml:math id="M377" display="inline"><mml:msup><mml:mi/><mml:mi mathvariant="normal">b</mml:mi></mml:msup></mml:math></inline-formula> low channel with <inline-formula><mml:math id="M378" display="inline"><mml:mi mathvariant="italic">γ</mml:mi></mml:math></inline-formula> correction.</p></table-wrap-foot></table-wrap>

</sec>
<sec id="Ch1.S3.SS2.SSS3">
  <label>3.2.3</label><title>Quantifying sea-ice growth rates</title>
      <p id="d1e7247">The growth rate of sea ice depends on the balance of fluxes at the sea-ice–ocean interface, the thermal conductivity of the sea ice (<inline-formula><mml:math id="M397" display="inline"><mml:mi>K</mml:mi></mml:math></inline-formula>), sea-ice thickness <inline-formula><mml:math id="M398" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, and the absolute sea-ice surface temperature (<inline-formula><mml:math id="M399" display="inline"><mml:mrow><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">s</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>). Growth rates in young sea ice have been observed to range from 2.7 to 12 cm d<inline-formula><mml:math id="M400" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <xref ref-type="bibr" rid="bib1.bibx54" id="paren.95"/>. Following <xref ref-type="bibr" rid="bib1.bibx45" id="text.96"/>, the change in sea-ice thickness is
              <disp-formula id="Ch1.E16" content-type="numbered"><label>16</label><mml:math id="M401" display="block"><mml:mrow><mml:mstyle displaystyle="true"><mml:mfrac style="display"><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow><mml:mrow><mml:mi mathvariant="normal">d</mml:mi><mml:mi>t</mml:mi></mml:mrow></mml:mfrac></mml:mstyle><mml:mo>=</mml:mo><mml:mo>-</mml:mo><mml:msub><mml:mi>F</mml:mi><mml:mtext>C</mml:mtext></mml:msub><mml:mo>/</mml:mo><mml:mo>(</mml:mo><mml:msub><mml:mi mathvariant="italic">ρ</mml:mi><mml:mtext>i</mml:mtext></mml:msub><mml:mi>L</mml:mi><mml:mo>)</mml:mo><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where <inline-formula><mml:math id="M402" display="inline"><mml:mi>L</mml:mi></mml:math></inline-formula> gives the latent heat of fusion of water, and <inline-formula><mml:math id="M403" display="inline"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mtext>C</mml:mtext></mml:msub></mml:mrow></mml:math></inline-formula> gives the vertical conductive heat flux through the sea ice. <inline-formula><mml:math id="M404" display="inline"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mtext>C</mml:mtext></mml:msub></mml:mrow></mml:math></inline-formula>, was calculated using
              <disp-formula id="Ch1.E17" content-type="numbered"><label>17</label><mml:math id="M405" display="block"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mtext>C</mml:mtext></mml:msub><mml:mo>=</mml:mo><mml:mi>K</mml:mi><mml:mo>(</mml:mo><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">s</mml:mi></mml:msub><mml:mo>-</mml:mo><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">f</mml:mi></mml:msub><mml:mo>)</mml:mo><mml:mo>/</mml:mo><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>.</mml:mo></mml:mrow></mml:math></disp-formula>
            The vertically averaged thermal conductivity is <inline-formula><mml:math id="M406" display="inline"><mml:mrow><mml:mi>K</mml:mi><mml:mo>=</mml:mo><mml:msub><mml:mi>K</mml:mi><mml:mtext>i</mml:mtext></mml:msub><mml:mo>(</mml:mo><mml:mn mathvariant="normal">1</mml:mn><mml:mo>-</mml:mo><mml:mi mathvariant="italic">ϕ</mml:mi><mml:mo>)</mml:mo><mml:mo>+</mml:mo><mml:msub><mml:mi>K</mml:mi><mml:mi mathvariant="normal">br</mml:mi></mml:msub><mml:mi mathvariant="italic">ϕ</mml:mi></mml:mrow></mml:math></inline-formula>, where the subscripts i and br indicate pure ice and brine, respectively. <inline-formula><mml:math id="M407" display="inline"><mml:mrow><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">f</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is given by the salinity-dependent freezing point of the ocean, and <inline-formula><mml:math id="M408" display="inline"><mml:mrow><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">s</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is found by iteratively solving the surface energy balance of the sea ice
              <disp-formula id="Ch1.E18" content-type="numbered"><label>18</label><mml:math id="M409" display="block"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mrow><mml:mi mathvariant="normal">L</mml:mi><mml:mo>↓</mml:mo></mml:mrow></mml:msub><mml:mo>-</mml:mo><mml:msub><mml:mi>F</mml:mi><mml:mrow><mml:mi mathvariant="normal">L</mml:mi><mml:mo>↑</mml:mo></mml:mrow></mml:msub><mml:mo>+</mml:mo><mml:msub><mml:mi>F</mml:mi><mml:mtext>S</mml:mtext></mml:msub><mml:mo>+</mml:mo><mml:msub><mml:mi>F</mml:mi><mml:mtext>C</mml:mtext></mml:msub><mml:mo>=</mml:mo><mml:mn mathvariant="normal">0</mml:mn><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where sensible and latent heat fluxes have been neglected because the wind speed in our experiments was less than 0.1 m s<inline-formula><mml:math id="M410" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>. We parameterised the downwelling longwave flux, <inline-formula><mml:math id="M411" display="inline"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mrow><mml:mi mathvariant="normal">L</mml:mi><mml:mo>↓</mml:mo></mml:mrow></mml:msub></mml:mrow></mml:math></inline-formula>, following <xref ref-type="bibr" rid="bib1.bibx7" id="text.97"/>, as
              <disp-formula id="Ch1.E19" content-type="numbered"><label>19</label><mml:math id="M412" display="block"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mrow><mml:mi mathvariant="normal">L</mml:mi><mml:mo>↓</mml:mo></mml:mrow></mml:msub><mml:mo>=</mml:mo><mml:mi mathvariant="italic">σ</mml:mi><mml:mo>(</mml:mo><mml:mn mathvariant="normal">0.746</mml:mn><mml:mo>+</mml:mo><mml:mn mathvariant="normal">0.0066</mml:mn><mml:mo>(</mml:mo><mml:mi mathvariant="italic">ψ</mml:mi><mml:mo>/</mml:mo><mml:mrow class="unit"><mml:mi mathvariant="normal">mbar</mml:mi></mml:mrow><mml:mo>)</mml:mo><mml:mo>)</mml:mo><mml:msubsup><mml:mi>T</mml:mi><mml:mi mathvariant="normal">a</mml:mi><mml:mn mathvariant="normal">4</mml:mn></mml:msubsup><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where <inline-formula><mml:math id="M413" display="inline"><mml:mi mathvariant="italic">σ</mml:mi></mml:math></inline-formula> is the Stefan–Boltzmann constant, <inline-formula><mml:math id="M414" display="inline"><mml:mi mathvariant="italic">ψ</mml:mi></mml:math></inline-formula> is the water vapour pressure, and <inline-formula><mml:math id="M415" display="inline"><mml:mrow><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">a</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> is the absolute temperature of the atmosphere. The upwelling longwave flux, <inline-formula><mml:math id="M416" display="inline"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mrow><mml:mi mathvariant="normal">L</mml:mi><mml:mo>↑</mml:mo></mml:mrow></mml:msub></mml:mrow></mml:math></inline-formula>, is taken to be
              <disp-formula id="Ch1.E20" content-type="numbered"><label>20</label><mml:math id="M417" display="block"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mrow><mml:mi mathvariant="normal">L</mml:mi><mml:mo>↑</mml:mo></mml:mrow></mml:msub><mml:mo>=</mml:mo><mml:mi mathvariant="italic">σ</mml:mi><mml:mi mathvariant="italic">ϵ</mml:mi><mml:msubsup><mml:mi>T</mml:mi><mml:mi mathvariant="normal">s</mml:mi><mml:mn mathvariant="normal">4</mml:mn></mml:msubsup><mml:mo>,</mml:mo></mml:mrow></mml:math></disp-formula>
            where the emissivity of sea ice is <inline-formula><mml:math id="M418" display="inline"><mml:mrow><mml:mi mathvariant="italic">ϵ</mml:mi><mml:mo>=</mml:mo><mml:mn mathvariant="normal">0.99</mml:mn></mml:mrow></mml:math></inline-formula>. We compare the growth rate of our experimental sea ice to natural sea ice by modelling and measuring the growth rate of the four experimental runs presented in Sect. <xref ref-type="sec" rid="Ch1.S3.SS2.SSS1"/> and Table <xref ref-type="table" rid="Ch1.T2"/> (Fig. <xref ref-type="fig" rid="Ch1.F12"/>). When the temperature of a sensor at depth <inline-formula><mml:math id="M419" display="inline"><mml:mi>z</mml:mi></mml:math></inline-formula> dipped by <inline-formula><mml:math id="M420" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.1</mml:mn><mml:msup><mml:mi/><mml:mi mathvariant="normal">o</mml:mi></mml:msup></mml:mrow></mml:math></inline-formula>C below the water temperature for 30 min, we took <inline-formula><mml:math id="M421" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub><mml:mo>=</mml:mo><mml:mi>z</mml:mi></mml:mrow></mml:math></inline-formula>. For each run, we forced the sea-ice growth and desalination model (Sect. <xref ref-type="sec" rid="Ch1.S3.SS2.SSS2"/>, <xref ref-type="bibr" rid="bib1.bibx49" id="altparen.98"/>) with the mean <inline-formula><mml:math id="M422" display="inline"><mml:mrow><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">a</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> and <inline-formula><mml:math id="M423" display="inline"><mml:mi mathvariant="italic">ψ</mml:mi></mml:math></inline-formula> during the freezing period, as measured by the weather station (Table <xref ref-type="table" rid="Ch1.T2"/>). Incoming shortwave flux, <inline-formula><mml:math id="M424" display="inline"><mml:mrow><mml:msub><mml:mi>F</mml:mi><mml:mtext>S</mml:mtext></mml:msub></mml:mrow></mml:math></inline-formula>, was taken to be 8 W m<inline-formula><mml:math id="M425" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> based on measurements of PAR just above the sea-ice surface (Sect. <xref ref-type="sec" rid="Ch1.S3.SS1.SSS3"/>). In this case, Eqs. (<xref ref-type="disp-formula" rid="Ch1.E16"/>) to (<xref ref-type="disp-formula" rid="Ch1.E20"/>) were used to calculate <inline-formula><mml:math id="M426" display="inline"><mml:mrow><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">s</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, <inline-formula><mml:math id="M427" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, and the internal sea-ice temperature profile. <inline-formula><mml:math id="M428" display="inline"><mml:mrow><mml:msub><mml:mi>T</mml:mi><mml:mi mathvariant="normal">f</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> was calculated from the salinity-dependent freezing point of the model ocean.</p>

      <?xmltex \floatpos{t}?><fig id="Ch1.F12"><?xmltex \currentcnt{12}?><?xmltex \def\figurename{Figure}?><label>Figure 12</label><caption><p id="d1e7805">Thickness measured from temperature profiles during the experiments presented in Sect. <xref ref-type="sec" rid="Ch1.S3.SS1.SSS3"/> and modelled using Eq. (<xref ref-type="disp-formula" rid="Ch1.E16"/>).</p></caption>
            <?xmltex \igopts{width=199.169291pt}?><graphic xlink:href="https://amt.copernicus.org/articles/14/1833/2021/amt-14-1833-2021-f12.png"/>

          </fig>

      <p id="d1e7818">Over the four experiments, measured growth rates range from 2 cm d<inline-formula><mml:math id="M429" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (experiment 3, highest air temperature) to 4 cm d<inline-formula><mml:math id="M430" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">1</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> (experiment 2, lowest air temperature). These growth rates are within the range of those reported by <xref ref-type="bibr" rid="bib1.bibx54" id="text.99"/>. Our growth rates are at the low end of this range, because the low wind speed in our facility effectively removes the latent and sensible heat fluxes present in the field. The change in <inline-formula><mml:math id="M431" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> with time is not linear; rather, as has been observed before <xref ref-type="bibr" rid="bib1.bibx1" id="paren.100"/>, the rate of increase in <inline-formula><mml:math id="M432" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula> decreases with increasing <inline-formula><mml:math id="M433" display="inline"><mml:mrow><mml:msub><mml:mi>h</mml:mi><mml:mi mathvariant="normal">si</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>. Modelled thickness captures the non-linearity in the measured growth rates and the order of the growth rates, with coldest temperatures producing fastest growth. Modelled thickness deviates from the measurements by up to 3 cm.</p>
      <?pagebreak page1847?><p id="d1e7886">Modelling thickness in this way is useful for planning experiments but – considering temperature profiles are measured during each experiment – measuring temperature and thickness gives better precision. Growth rates in the RvG-ASIC are within the range of those measured in the field and are in agreement with thermodynamic modelling <xref ref-type="bibr" rid="bib1.bibx45" id="paren.101"/>.</p>
</sec>
</sec>
</sec>
<sec id="Ch1.S4" sec-type="conclusions">
  <label>4</label><title>Conclusions</title>
      <p id="d1e7902">We have described the Roland von Glasow Air-Sea-Ice Chamber (RvG-ASIC) and the suite of instruments supporting it, and we have given an overview of the protocols used to run experiments in the facility. We presented technical results from experiments in the facility showing (1) the time constant for air exchanging between our sealed chamber and the outside is (<inline-formula><mml:math id="M434" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.66</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.07</mml:mn></mml:mrow></mml:math></inline-formula>) d; (2) the time constant for mixing our tank is (<inline-formula><mml:math id="M435" display="inline"><mml:mrow><mml:mn mathvariant="normal">4.2</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.1</mml:mn></mml:mrow></mml:math></inline-formula>) min; (3) the integrated irradiance of UV-A and PAR at 0 <inline-formula><mml:math id="M436" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C are 5.6 and 26.4 W m<inline-formula><mml:math id="M437" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula>, respectively; (4) the temperature sensitivity of our LED and UV-A lighting is (<inline-formula><mml:math id="M438" display="inline"><mml:mrow><mml:mn mathvariant="normal">0.028</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.003</mml:mn></mml:mrow></mml:math></inline-formula>) W m<inline-formula><mml:math id="M439" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> and <inline-formula><mml:math id="M440" display="inline"><mml:mrow><mml:mo>(</mml:mo><mml:mn mathvariant="normal">0.16</mml:mn><mml:mo>±</mml:mo><mml:mn mathvariant="normal">0.01</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:math></inline-formula> W m<inline-formula><mml:math id="M441" display="inline"><mml:msup><mml:mi/><mml:mrow><mml:mo>-</mml:mo><mml:mn mathvariant="normal">2</mml:mn></mml:mrow></mml:msup></mml:math></inline-formula> <inline-formula><mml:math id="M442" display="inline"><mml:msup><mml:mi/><mml:mo>∘</mml:mo></mml:msup></mml:math></inline-formula>C, respectively; and (5) PAR intensity varies by around 10 % near the centre of the tank but is as low as 60 % near the corners. These technical results can be used to design and interpret future experiments. We also characterised our experimental sea ice showing that (6) the extinction coefficient of PAR in our experimental sea ice is within the range of previously observed PAR extinction coefficients in young sea ice; (7) the bulk salinity of our experimental sea ice is similar to that observed in nature and is in agreement with halo-dynamic modelling; and (8) the growth rate of our experimental sea ice is within the range of previously reported growth rates and is in reasonable agreement with thermodynamic modelling. This characterisation builds confidence that the RvG-ASIC produces experimental sea ice that is a reasonable analogue of natural sea ice for these important parameters.</p>
      <p id="d1e8012">The RvG-ASIC is a powerful and versatile tool for studying sea ice and has potential to investigate physics, chemistry, and biology. It is best suited to process studies, bridging the gap between numerical models and reality. The facility was named in honour of its founder, who won funding for the facility, led its design and construction, but sadly died in September 2015 before it could be put into full use.</p>
</sec>

      
      </body>
    <back><notes notes-type="codedataavailability"><title>Code and data availability</title>

      <p id="d1e8020">All data, plot scripts, and model code used to produce this article are provided as supplementary information accessible at <uri>https://doi.org/10.5281/zenodo.4419170</uri> <xref ref-type="bibr" rid="bib1.bibx48" id="paren.102"/>.</p>
  </notes><notes notes-type="authorcontribution"><title>Author contributions</title>

      <p id="d1e8032">MTh prepared the article with JF, JK, and DN. All authors were involved in some of the experimental work. MTh did the modelling. JF managed the facility from 2015 to 2018, and OC managed the facility from 2018 to 2020, both under the supervision of JK.</p>
  </notes><notes notes-type="competinginterests"><title>Competing interests</title>

      <p id="d1e8038">The authors declare that they have no conflict of interest.</p>
  </notes><notes notes-type="sistatement"><title>Special issue statement</title>

      <p id="d1e8044">This article is part of the special issue “Simulation chambers as tools in atmospheric research (AMT/ACP/GMD inter-journal SI)”. It is not associated with a conference.</p>
  </notes><ack><title>Acknowledgements</title><p id="d1e8050">Roland von Glasow was instrumental in the design, construction, and scientific vision of the facility. Thanks to Bill Sturges, Dorothee Bakker, Martin Vancoppenolle, and Finlo Cottier for their time and scientific input to the RvG-ASIC. Jeremey Wilkinson and Martin King provided much useful advice and loaned us equipment. Thanks also to the technical support at UEA: Andy Macdonald, Stuart Rix, Dave Blomfield, Nick Griffin, Gareth Flowerdue, Ben McLeod, and Nick Garrard. This work received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7-2007-2013, grant agreement no. 616938) and the Horizon 2020 research and innovation programme through the EUROCHAMP-2020 Infrastructure Activity under grant agreement no. 730997, as well as the University of East Anglia. Oliver Tooth, and Mathilde Tranter were supported by an internship granted by the Environmental Sciences department at UEA.</p></ack><notes notes-type="financialsupport"><title>Financial support</title>

      <?pagebreak page1848?><p id="d1e8055">This research has been supported by the European Research Council, Seventh Framework Programme (FP7 Ideas (grant no. 616938)) and the H2020 Research Infrastructure EUROCHAMP-2020 (grant no. 730997).</p>
  </notes><notes notes-type="reviewstatement"><title>Review statement</title>

      <p id="d1e8061">This paper was edited by Hartmut Herrmann and reviewed by Brice Loose and one anonymous referee.</p>
  </notes><ref-list>
    <title>References</title>

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    <!--<article-title-html>The Roland von Glasow Air-Sea-Ice Chamber (RvG-ASIC): an experimental facility for studying ocean–sea-ice–atmosphere interactions</article-title-html>
<abstract-html><p>Sea ice is difficult, expensive, and potentially dangerous to observe in nature. The remoteness of the Arctic Ocean and Southern Ocean complicates sampling logistics, while the heterogeneous nature of sea ice and rapidly changing environmental conditions present challenges for conducting process studies. Here, we describe the Roland von Glasow Air-Sea-Ice Chamber (RvG-ASIC), a laboratory facility designed to reproduce polar processes and overcome some of these challenges. The RvG-ASIC is an open-topped 3.5&thinsp;m<sup>3</sup> glass tank housed in a cold room (temperature range: −55 to +30&thinsp;°C). The RvG-ASIC is equipped with a wide suite of instruments for ocean, sea ice, and atmospheric measurements, as well as visible and UV lighting. The infrastructure, available instruments, and typical experimental protocols are described.</p><p>To characterise some of the technical capabilities of our facility, we have quantified the timescale over which our chamber exchanges gas with the outside, <i>τ</i><sub>l</sub> = (0.66±0.07)&thinsp;d, and the mixing rate of our experimental ocean, <i>τ</i><sub>m</sub> = (4.2±0.1)&thinsp;min. Characterising our light field, we show that the light intensity across the tank varies by less than 10&thinsp;% near the centre of the tank but drops to as low as 60&thinsp;% of the maximum intensity in one corner. The temperature sensitivity of our light sources over the 400 to 700&thinsp;nm range (PAR) is (0.028±0.003)&thinsp;W&thinsp;m<sup>−2</sup>&thinsp;°C<sup>−1</sup>, with a maximum irradiance of 26.4&thinsp;W&thinsp;m<sup>−2</sup> at 0&thinsp;°C; over the 320 to 380&thinsp;nm range, it is (0.16±0.1)&thinsp;W&thinsp;m<sup>−2</sup>&thinsp;°C<sup>−1</sup>, with a maximum irradiance of 5.6&thinsp;W&thinsp;m<sup>−2</sup> at 0&thinsp;°C.</p><p>We also present results characterising our experimental sea ice. The extinction coefficient for PAR varies from 3.7 to 6.1&thinsp;m<sup>−1</sup> when calculated from irradiance measurements exterior to the sea ice and from 4.4 to 6.2&thinsp;m<sup>−1</sup> when calculated from irradiance measurements within the sea ice. The bulk salinity of our experimental sea ice is measured using three techniques, modelled using a halo-dynamic one-dimensional (1D) gravity drainage model, and calculated from a salt and mass budget. The growth rate of our sea ice is between 2 and 4&thinsp;cm&thinsp;d<sup>−1</sup> for air temperatures of ( − 9.2±0.9)&thinsp;°C and ( − 26.6±0.9)&thinsp;°C. The PAR extinction coefficients, vertically integrated bulk salinities, and growth rates all lie within the range of previously reported comparable values for first-year sea ice. The vertically integrated bulk salinity and growth rates can be reproduced well by a 1D model. Taken together, the similarities between our laboratory sea ice and observations in nature, as well as our ability to reproduce our results with a model, give us confidence that sea ice grown in the RvG-ASIC is a good representation of natural sea ice.</p></abstract-html>
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