The interpretation of simulation chamber experiments requires precise characterisation of the physical state of the chamber. This includes temperature, relative humidity and water vapour mixing ratio as well as pressure and radiation intensity. Radiation must be continuously monitored in outdoor chambers, whereas parametrisations derived from regular characterisation experiments are often sufficient when lamps are used (Sect. ). A large number of different instruments are used to measures the constituents in the air during chamber experiments. In most chambers, gas-phase inorganic species including ozone (O3), nitrogen oxides (NO and NO2), total reactive nitrogen oxides (NOy), and carbon monoxide (CO) are monitored as they are fundamental for understanding atmospheric chemical processes. Sulfur dioxide (SO2) is also frequently measured, and in some cases nitrous acid (HONO) , which is an important precursor for OH radicals and is released from Teflon film . For the detection of volatile organic compounds (VOCs), Proton-Transfer-Reaction Time-of-Flight Mass-Spectrometry (PTR-ToF-MS) is commonly employed. Many chambers are additionally equipped with advanced mass spectrometers using various chemical ionisation schemes. Some facilities have Fourier-transform infrared (FTIR) and tunable diode laser (TDL) spectrometers installed, which provide measurements of a wide range of inorganic and organic species. Measurements of radical species, such as hydroxyl radicals (OH) and nitrate radicals (NO3) , which are two major atmospheric oxidants, are also available in some chambers.

Condensation particle counters (CPCs) and scanning mobility particle sizers (SMPS) are standard instruments used to determine number concentration and size distribution of aerosol particles. Aerosol chemical composition is most often analysed using an Aerosol Mass Spectrometer (AMS) or Chemical Ionisation Mass Spectrometers (CIMS) with specialised aerosol inlets, such as FIGAERO (Filter Inlet for Gas and Aerosols, Aerodyne) or CHARON (CHemical Analysis of aeRosol ON-line, Ionicon). The range of detectable species can be further expanded by employing different ionization methods. Filter sampling and offline analysis using various advanced methods is also often conducted to derive the chemical composition of particles. Other chambers, such as the CESAM and FORTH chambers, are equipped with instruments to measure the optical and hygroscopic properties of aerosols to retrieve their direct and indirect radiative forcing effects. Some chambers are equipped with instruments to measure ice-nucleating particles, cloud droplets, and ice crystals.

The instrumentation available at the AIDA, LACIS-T and RvG chambers differs from that at other facilities due to their specific research objectives. For cloud simulation experiments, the AIDAd and AIDAc2 chambers are equipped with special instruments to measure droplet and ice crystal number concentrations and size distributions, as well as the habit and polar scattering phase functions of ice crystals. Furthermore, specialised sampling lines and instruments are used for measuring total aerosol, interstitial aerosol, as well as droplet and ice crystal residuals. LACIS-T is specialised in experiments on the interaction between aerosols, clouds and turbulence. It is therefore equipped with instruments for the detailed characterisation of microphysical properties, as well as thermodynamic and flow conditions with high spatial and temporal resolution. The RvG-ASIC chamber focuses on experiments addressing interactions at the ice–water–atmosphere interface and, in addition to standard gas-phase instrumentation, includes instruments for characterising ice and water properties, such as water conductivity and liquid–ice volume fraction.

All chambers offer the opportunity to attach specialised additional instruments depending on the needs of the research topic. This particularly also allows users to bring their own instruments to the chambers. Some of the additional key instruments are listed in the chamber descriptions below.

ACD-C (Atmospheric Chemistry Department Chamber) is a twin-chamber at the Leibniz Institute for Tropospheric Research (TROPOS) in Leipzig, Germany . Studies in the ACD-C chamber focus on VOC degradation, SOA formation, particulate product identification, multiphase chemistry, and chemical processing in deliquescent particles. The cylindrical chambers are made of Teflon FEP (fluorinated ethylene propylene) film (volume: 19 m3). The top of the chamber is fixed to a movable frame that enables the chamber to collapse while air is sampled by instruments during an experiment. This enables the pressure to be kept constant and the chamber can be operated in batch mode even for high sampling rates of instruments. The chamber is enclosed in a temperature-controlled housing.

Between experiments, the chamber is constantly flushed with clean air (flow rate: 200 Lmin-1). To humidify the air, Milli-Q water is sprayed and evaporated in a heated part of the central inlet tube (300 °C). The Leipzig Biomass Burning Facility (LBBF) is part of ACD-C facility allowing biomass burning emissions to be transferred into the chamber. Furthermore, the laboratory security level has been recently certified (German S2 standard) to enable chamber experiments with biological agents.

Key instruments include the measurement of gas-phase organic species by mass spectrometry using different ionisation methods and by gas-chromatography. Physical and chemical properties of aerosol particles are analysed by online and offline instruments including mass spectrometers with specialised aerosol inlets (EESI, FIGAERO) and an Orbitrap detector.

The ACEX (Atmospheric Chemistry Experimental Chamber) facility was built in Copenhagen, Denmark, based on a steady-state design . It consists of a 4.5 m3 Teflon bag mounted inside a temperature-controlled, insulated room of walk-in size (Viessmann A/S). The chamber is operated in a constant flow mode using mass flow controllers for gases and a syringe pump (NE-300, New Era Pump Systems Inc.) for continuous injection and evaporation of reactants in a warmed glass bulb. The ACEX photochemical reactor is a 125 L quartz tube with multipass mirrors for FTIR spectroscopy . Other key instruments include the measurement of gas-phase species by various mass spectrometer instruments, gas-chromatography and cavity ring-down spectroscopy. Aerosol physical properties are analysed by various instruments including a cloud condensation nucleation (CCN) counter and a neutral cluster and air ion spectrometer (NAIS).

The AIDA (Aerosol Interactions and Dynamics in the Atmosphere) facility at the Karlsruhe Institute of Technology (KIT), Germany, consists of several chambers and laboratories for the study of trace gas, aerosol and cloud processes at a wide range of temperatures, pressures (from atmospheric pressure to below 1 hPa) and relative humidities (0 %–100 % with respect to liquid water above 0 °C or with respect to ice below 0 °C). The AIDA chambers can also be operated as expansion-type cloud simulation chambers to investigate cloud microphysical processes in mixed-phase and cirrus clouds . Increasing relative humidities and supersaturations are achieved by controlled pumping and pressure reduction, thereby simulating the conditions for cloud formation in rising air parcels of convective or lee wave clouds.

The classic cloud simulation chamber AIDAc was in operation from 1997–2024 with a comprehensive set of trace gas, aerosol, droplet and ice crystal instruments for a variety of studies on heterogeneous chemistry , aerosol optical properties , secondary organic aerosol formation , polar stratospheric clouds , and primary ice formation processes . The AIDAc chamber was made of aluminium and constructed as a vacuum vessel, which could be evacuated to below 1 hPa for both experimental and cleaning purposes. It is currently replaced by the new AIDAc2 chamber (cold and clean) which is a unique user facility for aerosol-cloud-climate research including photochemical processes and new particle formation at temperatures down to -100°C. The AIDAc2 chamber is made of high-quality stainless steel with electro-polished inner surfaces, metal-sealed flanges and components enabling experiments to be conducted under ultra-clean conditions, low pressures, and low temperatures.

The new dynamic cloud simulation chamber AIDAd came into operation in 2020 for warm and mixed-phase cloud experiments. Its unique active wall temperature control allows for adiabatic expansion runs with well-controlled constant cooling rates between 0.1 and 10 Kmin-1. AIDAd can simulate convective cloud or storm conditions with updraft velocities of up to 15 ms-1.

A third chamber called AIDAs (for “service”) came into operation in 2026 as part of the ACTRIS Centre for Cloud In Situ Measurements (CIS). This facility will mainly be used for instrument calibration, training, and innovative method developments for in situ measurements of ice-nucleating particles, cloud droplets and ice crystals, as well as cloud water chemistry. These activities are key responsibilities of CIS as part of the pan-European Aerosol, Clouds and Trace Gases Research Infrastructure ACTRIS.

AURA (Aarhus University Research on Aerosols) is a cuboid FEP Teflon chamber (1.63m×1.63m×1.85m) at Aarhus University, Denmark . AURA is used for simulations of aerosol formation and ageing. The chamber bag is inside a temperature controlled room, allowing also for temperature ramps during an experiment . Five sensors around the chamber monitor the temperature. The cold room is inside an air-conditioned laboratory providing stable conditions for the instrumentation coupled to AURA. The relative humidity of the air can be controlled. The AURA chamber can be either operated with and without a replenishment flow to compensate for the air consumption of instruments. Seed particles can be introduced into AURA from using standard atomizers, custom made sea spray simulation tanks for aerosol generation mediated by bubbles , and a combustion chamber designed for candles .

Organic gas-phase species can be measured by gas-chromatography and a PTR-ToF-MS instrument, which is also equipped with an CHARON inlet for the analysis of aerosol chemical composition. Different types of filter samples can be acquired, e.g., for off-line chemical analysis or electron microscopy. In addition, aerosol physical properties are analysed including their hygroscopicity.

The CESAM facility at the Laboratoire Interuniversitaire des Systèmes Atmosphériques (LISA) consists of two chambers (CESAM and CSA). They are used for gas-phase photochemistry and multiphase chemistry studies involving gaseous trace species, organic particles, water droplets, mineral dust, soot, and salts. In addition, living organism can be exposed to pollution for determining health impacts. Cloud events can also be simulated in CESAM.

The CESAM chamber (Chambre de Simulation Atmosphèrique Multiphasique) is a stainless-steel reactor with a volume of 4.2 m3, which can be evacuated to a few 10-4hPa and can be temperature controlled . Due to the very low level of electrostatic charges on its walls, the aerosol lifetime in CESAM is very long up to 4 d for 200 nm diameter particles, enabling the study of aerosol ageing processes and their impact on aerosol properties, such as their chemical composition, optical absorption, and scattering and hygroscopic properties e.g.,.

The second chamber, CSA, is dedicated to atmospheric gas-phase process studies and spectroscopic studies . It is a 1 m3 Pyrex reactor complementing the steel chamber. It can be evacuated to a few 10-3hPa and is currently being updated to be equipped with high-pressure Xenon lamps. The CSA chamber is well suited to investigate kinetics and chemical mechanisms governing the day- and nighttime degradation of organic pollutants e.g.,.

Various instruments at these chambers measure gas-phase species including nighttime oxidants (NO3) and its reservoir species dinitrogen pentoxide (N2O5). VOCs are measured using FT-IR/UV-VIS spectroscopy, cavity-based absorption spectroscopy and various mass spectrometer instruments. A focus of the analysis of particles is on their optical properties, hygroscopicity and their chemical composition, which are measured using online and offline instruments.

ChAMBRe (Chamber for Aerosol Modelling and Bio-aerosol Research) at the National Institute of Nuclear Physics in Genoa (Italy) is managed in collaboration with the Environmental Physics Laboratory at the Physics Department of the University of Genoa. The main focus is on bioaerosol research , but it can also be used for various other purposes , such as the investigation of carbonaceous aerosols , and studies on vegetable applications and aged aerosol for determining their oxidative potential and toxicity . Key instruments include aerosol particle sizer (APS), aerosol mass spectrometer (ACMS) and instruments for the measurement of aerosol optical properties.

ChAMBRe is a stainless steel cylinder (volume: 2.2 m3, diameter: 2.9 m, maximum height: 2.9 m). On the side, a smaller horizontal cylinder is connected to the main volume through a pneumatic valve. This volume contains a semi-automatic, movable shelf that allows the insertion of samples into the chamber. The particle lifetime ranges from a few hours to about 1 d depending on the particle size.

The ESC-Q-UAIC (Environmental Simulation Chamber-made by Quartz from the University Alexandru Ioan Cuza in Iasi) chamber is part of the Integrated Centre of Environmental Science Studies in the North-Eastern Development Region (CERNESIM) located in Iasi, Romania . Research at this facility focuses on gas-phase reaction kinetics and studies of gas-phase reaction oxidation products. The chamber consists of three quartz glass tubes with a total length of 4.2 m and an inner diameter of 0.48 m. The tubes are sealed with Teflon-coated aluminium flanges at both ends and are supported by a metal frame. The chamber can be evacuated to a pressure of 6.5×10-2hPa. The ESC-Q-UAIC chamber operates in a temperature-controlled environment.

The facility also includes two complementary Teflon chambers, each with a volume of approximately 10 m3. They are part of the Laboratory of Applied Chemistry in Atmospheric Aerosol Science (RA-01) at the RECENT AIR Centre. The MICSAAC chamber (Medium Isobaric Chamber for Atmospheric Chemistry Simulation and Analysis) is designed for controlled laboratory studies of aerosol formation, multiphase chemical reactions, reaction kinetics, and the evolution of aerosol properties from diverse sources (e.g., engine combustion and industrial emissions). Its versatility also enables the testing of photocatalytic depolluting materials and the study of the photodegradation of agricultural products such as pesticides. The second Teflon chamber, PRO-TRACE-01, is Romania's first mobile atmospheric simulation chamber dedicated to in situ investigations of secondary organic aerosol formation. This 10 m3 chamber is mounted on a mobile platform, enabling real-time measurements directly in the field.

EUPHORE (European Photoreactor) at the CEAM Foundation in Valencia, Spain, consists of two outdoor half-spherical chambers each having a volume of 200 m3 The large size and the use of natural light enable atmospheric studies of chemical processes under near-real conditions. The chambers are made of FEP Teflon film. The chambers have retractable covers that protect them and allow for experiments to be carried out in darkness and with ambient light exposure. To prevent significant heating of the air from solar radiation, the bottom of the chamber is cooled by refrigeration systems. The pressure is kept approximately at 100 Pa above ambient pressure.

In addition to single-compound experiments, the facility allows the introduction of complex emission mixtures (e.g., biomass burning or car exhaust) as well as VOCs with relatively low vapour pressures (e.g., pesticides), using dedicated injection and conditioning procedures. The high volume of EUPHORE allows the installation and test of depolluting or reactive materials.

EUPHORE is equipped with a wide range of analytical instruments for the chemical characterization of gases and aerosols, including the measurement of radicals (OH, HO2). These include different monitors, optical systems, chromatographic techniques, mass spectrometers, particulate matter instruments and systems to monitor physical parameters and optical aerosol properties. Both well-established and state-of-the-art techniques are used, notably PTR-ToF-MS, API-ToF-CIMS, ToF-ACSM-X, aethalometer, and a total carbon analyser.

The FORTH facility includes an indoor chamber (FORTH-ASC) and a mobile chamber (FORTH-MSC). FORTH-ASC is a Teflon chamber at the Foundation for Research and Technology Hellas, Institute of Chemical Engineering Sciences (FORTH/ICE-HT) in Patras, Greece . It is a 10 m3 (2.2m×2.2m×2m) reactor located in a temperature-controlled room with walls covered with UV lights. One of the characteristic features of FORTH-ASC is its direct connection to a variety of emission sources (wood stove, pellet stove, diesel generator, gasoline engine, barbecue, etc.) located in a laboratory underneath the facility. The corresponding emissions can be diluted, cooled, and can be injected into the chamber.

The FORTH-MSC is a mobile chamber facility designed to study atmospheric processes using ambient air from the location, where the chamber is placed . Its default configuration includes 2 PTFE (polytetrafluoroethylene) chambers (volume: 1.5 m3) surrounded in a hemispherical arrangement by 60 UV light lamps. One chamber is used for the experiment and the second acts as reference. The chambers and the UV light structure is protected from weather by a custom-built tent. When weather conditions allow it, the top of the tent can be removed in order to use natural sunlight for experiments. Static charge elimination for the chambers is performed periodically using specialised fans (ionising air blower, model SL-001) minimizing particle wall losses. Sampling is performed alternatively between the 2 chambers prolonging the duration of an experiment of up to 4 h.

VOCs are measured by mass spectrometry as well as off-line gas chromatography (GC). An VOC mass spectrometry instrument is also equipped with an aerosol inlet to analyse their chemical composition. In addition, particles are analysed using various instruments including an AMS and instruments, measuring their optical properties.

HELIOS (cHambrE de simuLation atmosphérique à Irradiation naturel d'OrléanS) is a highly instrumented outdoor atmospheric simulation chamber in Orléans, France . The HELIOS chamber is a 90 m3 single-walled hemispheric volume constructed of FEP Teflon foil. Almost all wetted surfaces are of FEP foil, Teflon coated, or made of PTFE. Two hatches located at the chamber base allow access of personnel. The chamber is covered by a moveable shelter that is retracted when sunlight is required. The interior of this shelter is lined with 49 near-UV lamps, providing actinic-like radiation. Two compressors provide a constant zero air gas supply. A second indoor chamber facility (FEP Teflon, 7.3 m3) is part of the HELIOS facility, and can be interfaced with the same set of instruments.

Various mass spectrometer instruments analyse organic compounds in the gas-phase and in particles at the HELIOS facility. In addition, FT-IR and cavity-based absorption instruments are available which also measure the nighttime oxidant NO3 and its reservoir species N2O5.

The Irish Atmospheric Simulation Chamber (IASC) at University College Cork in Ireland is used to investigate atmospheric oxidation reactions and kinetic studies and also serves as a testbed for development and evaluation of new atmospheric measurement techniques . The chamber is a 27 m3 cuboid (4.4m×2.8m×2.2m) made of FEP Teflon foil attached to an aluminium frame. The chamber and frame are situated inside an air-conditioned enclosure. Under the frame there are two inflatable platforms that, when inflated act as a floor for the chamber, when deflated enable thermalised air to circulate all around the cuboid. The two longer side walls of the housing are fitted with UV-A and UV-B broadband lamps. The internal surface of the enclosure is covered in a highly reflective foil (Mylar C3 ADF) to maximise the UV photon flux in the chamber. The chamber is typically operated at a pressure of 10 Pa over ambient. Between experiments, the chamber is generally flushed permanently at 1000 Lmin-1.

Apart from commercial instruments for trace gas and aerosol detection, the chamber is equipped with 10 optical ports which enable custom-made open path cavity enhanced absorption measurements of several relevant atmospheric species.

The KASCs (Kuopio Atmospheric Simulation Chambers) facility at Kuopio, Finland, consists of the ILMARI infrastructure and the KASC1 chamber. ILMARI offers possibilities for studies on emissions and aerosol particles, their atmospheric effects and toxicological properties. It is a collapsible FEP Teflon chamber (volume 29 m3), which is attached to a moving top frame . The pressure inside the chamber can be controlled by adding or removing extra weights on the moving top frame. The overpressure inside the full chamber is around 10 Pa. Two arrays of blacklight lamps can be switched on in different combinations. The chamber is situated in a temperature-controlled room.

The laboratory is equipped with various biomass combustion appliances and reactors, chassis dynamometer for light duty vehicles, and nanoparticle reactors. The biomass combustion appliances include an open burning setup mimicking wildfires, stoves, pellet burners and a grate combustion reactor. The emissions can be diluted before being injected into the chamber. In addition, the laboratory is equipped with a high-volume photochemical oxidation flow reactor (PEAR). Living cells (air–liquid interface cell exposure system) and animals (exposure chamber) can be exposed to both fresh and aged emissions from different source, in order to study health-related toxicological responses.

The KASC1 chamber is similar to the ILMARI chamber but smaller (9 m3) and is also located in a temperature-controlled room. There are two arrays of blacklight lamps. The KASC1 chamber is mainly used for process studies on biogenic emissions and is often used for experiments using VOC emissions from real plants.

Various mass spectrometers partly equipped with aerosol inlets measure organic compounds. In addition, key aerosol instruments analyse their optical properties.

LACIS-T turbulent Leipzig Aerosol Cloud Interaction Simulator, is a turbulent moist-air, closed-loop wind tunnel at the Leibniz Institute for Tropospheric Research (TROPOS) in Leipzig, Germany. Experiments in LACIS-T are ideal to study the interactions between turbulence and cloud microphysical processes. In LACIS-T, three conditioned air flows (2 particle-free, 1 containing aerosol) are turbulently mixed (using an active turbulence grid) in the measurement section, which is a stainless-steel cuboid (2m×0.8m×0.2m). The flow rate (mean velocity between 0.5 and 2 ms-1), temperature (-40 to 25 °C) and humidity (dew point between -40 and 25 °C) of the two particle-free flows can be separately controlled. The aerosol flow, which contains size-selected, quasi monodisperse aerosol particles of known chemical composition, is fed into the mixing zone of the two particle-free air flows. Due to this procedure, microphysical processes such as particle deliquescence , droplet formation and growth and ice crystal formation can be studied in a turbulent environment with defined temperature and saturation fluctuations. In addition, experiments studying cloud entrainment and mixing can be conducted . After having passed the measurement section, the entire flow is dried and heated via an adsorption dehumidifying system, cleared from particles via two particle filters and eventually re-used.

LACIS-T is specialised in aerosol and cloud experiments in a turbulent environment and is therefore equipped with instruments for detailed characterisation of cloud microphysics.

The Manchester Aerosol Chamber MAC, at University of Manchester, UK, has been designed to investigate atmospheric processes and the climate and toxicological impacts of air pollutants. The chamber is an 18 m3 collapsible FEP Teflon cuboid (3m×2m×3m), supported by a central fixed frame and counter-weighted upper and lower frames and surrounded by an air-conditioned housing. A suite of lamps provides simulated solar irradiation that mimics closely the ambient actinic flux spectrum.

The facility has a wide range of ancillary components for injection of pollutants, including electro pneumatically valve-controlled large-bore inlets for automated injection from real pollutant sources (wood-burning stove, dynamometer with a range of engines, compressors and generators, cooking chamber, etc.). MAC can be connected to a variety of devices for investigation of health impacts, including cell exposures (using a Vitrocell continuous-flow air–liquid interface system) and human exposures under clinical trial conditions.

Specialised mass spectrometers using various ionisation methods analyse organic compounds in the gas- and particle phase. In addition, aerosol properties are analysed in detail including their size distribution and hygroscopicity.

PACS-3 (PSI Atmospheric Chemistry Simulation chamber) is a 9 m3 collapsable chamber made of FEP Teflon at the Paul-Scherrer Insitute in Villigen, Switzerland. The chamber is located in a temperature-controlled shipping container . Blacklights are present to simulate sunlight and are either used to investigate photolysis reactions in the aerosol phase or to photolyse HONO to produce OH radicals. The facility specializes in investigating complex emissions from various sources and is equipped with a burning platform that can be used to study various complex emission sources including emissions from residential wood stoves and open burning processes, cooking emissions, and idling emissions from vehicles. The burning platform can direct the emissions into the chamber via an injection diluter providing either a 10 or 100-fold dilution. The burning emissions can also be stripped of either a majority (>90%) of the gaseous emissions via charcoal denuders or removing the primary aerosol emissions via HEPA (High-Efficiency Particulate Air) filters.

Organic compounds in the gas- and particle phase are measured using mass spectrometer instruments with different ionisation methods and aerosol inlets (e.g., EESI inlet).

The QUAREC facility at University of Wuppertal, Germany, consists of two glass chambers (QUAREC ASC and DURREC) originally constructed to study gas-phase oxidation reactions . A large-volume indoor Teflon chamber (WUTASC) is currently under construction to complement the experimental capabilities.

The QUAREC (QUArtz REaCtor) chamber comprises two joint cylindrical quartz glass tubes (total length: 6.2 m, inner diameter: 0.47 m, volume: 1.080 m3). At both ends, it is sealed by metal flanges coated with vitreous enamel. The glass tube is surrounded by eight boxes, whose inner surface is coated with reflective steel sheets. These boxes contain up to 64 lamps, which can be switched on in pairs. The glass tube is part of a temperature-controlled enclosure. Experiments can be carried out at pressures between 100 and 1000 hPa. The glass tube is kept at a pressure of 10-4hPa when no experiment is performed.

DURREC is a borosilicate glass tube with a volume of 480 L surrounded by 32 fluorescent lamps. The pumping system consists of a rotary vane pump and a root pump for evacuating the chamber to a pressure of about 10-3hPa.

Measurements at QUAREC focus on the detection of gas-phase species using absorption spectrometers (e.g., FT-IR), mass spectrometry and GC-GC mass spectrometry instruments.

The Roland von Glasow Air–Sea–Ice Chamber (RvG-ASIC) is a coupled atmosphere–ocean–sea–ice simulation chamber at the University of East Anglia (UEA), UK, designed to investigate the role of first-year sea–ice in tropospheric chemistry. The temperature of the chamber can be controlled between -55 and +30 °C, with a stability of ±0.3 °C . The chamber can also be run in ocean–atmosphere mode, snow-atmosphere mode (no liquid water) or in dry mode for purely atmospheric investigations. It thus provides a platform for multi-disciplinary experiments on physical, chemical and biological interactions between atmosphere, ocean, ice, and snow. The chamber comprises a glass water tank (2.4m×1.4m×1.1m, volume: 3.5 m3) with a removable FEP film-enclosed volume above (2.4m×1.4m× (0.1–1 m), volume: 0.3–3.3 m3). The water tank is equipped with a heating system and a circulation pump. Controlled illumination is provided by sun-simulating LED bands.

Instruments focus on characterizing ice and water properties, such as water conductivity and liquid–ice volume fraction, but also standard instruments for the measurement of gas-phase species are available.

The SAPHIR facility at Forschungszentrum Jülich, Germany, includes 2 chambers: The large outdoor SAPHIR chamber and the smaller indoor SAPHIR-STAR chamber. The scientific focus of experiments is on understanding the chemical transformation of gas-phase and aerosol species. SAPHIR is a double-wall cylindrical chamber made of FEP Teflon film (length: 18 m, diameter: 5 m, volume: 270 m3), which is equipped with a shutter system that allows experiments to be carried out under natural sunlight conditions or in the dark . To prevent contamination of the air inside the chamber with ambient air through microleaks or diffusion, the pressure in SAPHIR is slightly higher than ambient pressure (25 Pa) and the volume between the 2 chamber films is permanently flushed with ultra-pure nitrogen. The high cleanliness and minimum wall effects allow to perform experiments at ambient concentrations of trace gases and chemical reactions times that are typical for the real atmosphere.

The SAPHIR facility includes a plant chamber (SAPHIR-PLUS), which enables emissions from up to six trees to be transferred into the outdoor chamber . The trees are housed in a Teflon bag inside a temperature-controlled ship container with an illumination system that mimics natural sunlight. In addition to experiments with tree emissions, experiments can be conducted with ambient air in SAPHIR by sampling it through an inlet line that this mounted on a 50 m-high tower.

The second chamber, SAPHIR-STAR, is a constantly stirred tank glass reactor of cylindrical shape (length: 2.5 m, inner diameter: 1 m, volume: 2 m3) with a typically residence time of air of 1 h . It is housed in a temperature-controlled room. UV-C lamps mounted in a quartz glass tube along the central axis of the glass reactor.

Instruments at the SAPHIR chamber include several mass spectrometer instruments using various ionisation methods and inlets, for example to separate gas-phase species by gas-chromatography and to analyse the chemical composition of particles (EESI, CHARON inlets). In addition, gas-chromatography detects gas-phase species. Radicals including OH, HO2, and RO2 are measured by laser-induced fluorescence. Measurement of OH reactivity, the inverse lifetime of the OH radical , complements the measurement of reactive species.

The ACTRIS research infrastructure aims to provide harmonised quality standards for measurements and data of short-lived atmospheric constituents. For monitoring stations (ACTRIS Observational Platforms), this means a pre-defined set of minimum instrumentation, standard operation and data processing procedures as well as regular instrument calibrations provided by the calibration centres (ACTRIS Topical Centres). In addition, scheduled or continuous provision of measurement data to the ACTRIS Data Centre is required . For simulation chambers, however, a major part of the quality assurance focuses on the ways in which experiments are performed, whereas requirements on the instruments depend on the research topic and the conditions during the experiment. Where feasible, the same guidelines and calibration procedures apply for those types of instruments covered by the service portfolio of the ACTRIS Topical Centres. However, chamber experiments typically require many additional instruments, for which quality assurance needs to be ensured at the chamber facility.

There are also several quality assurance aspects related to the chambers and their characterisation. These include: (1) knowledge and characterisation of potential chamber effects and of physical properties, both of which are needed for the interpretation of the experiments, (2) appropriate preparation of the chamber before the experiment is performed, (3) accurate execution and documentation of the experiment, (4) quality assurance and control of instruments not serviced by the ACTRIS Topical Centres. Evaluations are repeated at regular intervals. The quality assurance framework is presented in Fig. .

The requirements for the ACTRIS National Facility label for simulation chambers are:

Carrying out chamber experiments according to the quality standards described below.

ACTRIS simulation chambers require regular characterisation of the physical properties and chamber effects (Fig. ). The results should be made available alongside data from experiments in the ACTRIS Data Centre so that chamber effects can be accurately considered, when data are re-used. The monitored physical properties typically include temperature, pressure, dilution rates and intensity and spectrum of the radiation from sunlight or lamps (e.g, photolysis frequencies). The range of temperature and pressure varies between the chambers, as does the accuracy and precision required. Standard sensors such as Pt100 sensors for temperature measurements and capacitance manometers for pressure measurements are most commonly used. They need to be placed at representative locations in the chamber.

If the chamber is built or operated in such a way that its volume remains constant, a replenishment flow can be used to counteract the drop in pressure caused by air loss from small leaks and instruments sampling the air. This is particularly important for Teflon chambers, which are usually operated at slightly higher than ambient pressure in order to maintain their integrity. The replenishment of air results in the dilution of gases, vapours and particles in the chamber, which must be monitored. This is achieved either by monitoring the replenishment flow rate using mass flow meters or by calculating the dilution rate from the measured loss of a chemically inert tracer molecule such as sulfur hexafluoride (SF6), water vapour, or CO2. Chambers made of glass or steel may not use a replenishment flow so that the pressure drops during the experiment.

The spectrum and intensity of the radiation are needed to calculate photolysis frequencies. If lamps are used, they can be assumed to be relatively stable over the time of an experiment. However, they may change with temperature (depending on the type of lamp) and over years of use. If natural sunlight is used, the actinic flux must be monitored during the experiment. Special care must be taken with respect to the location of the sensor, as shading can change with solar zenith angle and thus affect the measurements. For example, in the SAPHIR chamber at Forschungszentrum Jülich, the actinic flux is measured outside of the chamber on the roof of a nearby building and the photolysis frequencies are calculated using a model that includes shading effects and the transmission of the chamber's Teflon film .

As the lamp characteristics and the transmission of radiation can change over time, the validity of photolysis frequencies (j) must be checked regularly (at least once a year) in dedicated reference experiments. Chemical actinometry is commonly used to determine the most versatile photolysis parameter, j(NO2), and involves photolysis of a high mixing ratio of NO2.

The quality of the NO2 and NO measurements in the ACTRIS simulation chambers is ensured by the services of the ACTRIS Centre for in situ gas-phase measurements (CiGAS). Ozone is typically measured using calibrated UV-photometers with accuracy in the low ppbv range.

The inner surfaces of chambers can alter the concentration and composition of gases and particles within, mainly through deposition of species on the chamber walls e.g.,. The exact values can vary with time as properties of the chamber wall can depend on the history of previous experiments. In addition, the loss rate of particles on the chamber walls depends not only on the material of the walls and electrostatic charges, but also on the sizes of the particles .

Emission of trace gases can also affect the chamber experiments e.g.,. The chemical conversion of species on chamber surfaces can also play a role . In chambers made of Teflon, nitrogen oxides and nitrous acid (HONO) can be formed in the presence of light and humidity . In addition, small, oxygenated molecules, such as formaldehyde (HCHO) and acetaldehyde (CH3CHO), are typically emitted from the chamber walls e.g.. The exact source strength depends on the volume-to-surface ratio of the chamber, humidity, physical parameters, the history of experiments and cleaning procedures, as the mechanism behind these sources is likely to be the adsorption and desorption of molecules. These species can also act as precursors for the photolytic production of hydroxyl radicals. It is therefore recommended to regularly determine the radical formation rate by observing the decay of a volatile organic compound that does not photolyse.

Reference experiments are regularly used to derive parametrisations of chamber effects, which allow these effects to be quantified and used to correct measurements or to be implemented in the modelling of experiments. These parametrisations can be referred to as an auxiliary mechanism, which is available for many chambers on the EUROCHAMP website (https://www.eurochamp.org/, last access: 15 February 2026) and will be provided on the ACTRIS Data Centre website (https://www.data.actris.eu, last access: 15 February 2026) in the future.

As the relevance depends strongly on the type and objective of the research, the exact frequency of carrying out the characterisation experiments varies. It is recommended to perform at least one characterisation experiment in a series of experiments or after properties are expected to have changed (e.g., exchange of lamps, specific cleaning procedures). The minimum frequency for ACTRIS chambers is once a year. Quantification of chamber sources can also be achieved as part of an experiment, for example by observing the increase in the concentration of a compound in the clean chamber before other reactants are injected e.g., or to observe the loss rate of species before chemical processes are started or after they have been stopped.

A prerequisite for a meaningful experiment is that the air in the chamber is free from particles and reactive gas-phase species at the start of the experiment (Fig. ). As the level of cleanliness needed for an experiment depends largely on its type and purpose, there is no general rule except that experiments must not be affected by previous residues.

The method of cleaning vary with the chamber design and depend on the history of experiments. Measures to remove gas-phase impurities and reduce chamber wall effects include, for example, flushing the chamber with dry or humidified clean air, evacuating the chamber followed by flushing and re-filling with clean air, or exposing it to oxidants (e.g., ozone, oxygen atoms, hydroxyl radicals). Cleaning using these procedures can be achieved as adsorption of water molecules on the chamber wall material compete with the adsorption of impurities and the oxidation of impurities can lead to more volatile compounds than the impurities themselves. Evacuation, increasing the chamber temperature while flushing the chamber with clean air and manual cleaning are also efficient cleaning procedures, but they are not applicable to all chambers. The cleanliness of the chamber should be documented at the start of the experiment by, for example, including measurements of potential impurities in the dataset submitted to the ACTRIS Data Centre.

Seed aerosols and various gas-phase species, which serve as reactants or precursors for oxidants during an experiment, are added to the chamber via standardized procedures. Guidelines how to use the different injection systems and methods are outlined in . Gas and vapours can either be added using mass flow controllers, or evaporated from liquids and solids in heated vessels, which allow the vapour to be transported into the chamber using a clean air flow. The lower the volatility of the compound, the more consideration must be given to potential losses and adsorption and desorption in the inlet system. Short, potentially heatable inlet lines are advantageous.

Seed aerosols can be generated by an electrospray or collision atomiser, by condensation of heated gases, or by producing secondary organic aerosol from the oxidation of injected precursors. Various more specialised methods exist for the injection of particles such as of mineral dust, soot particles, bioaerosol, sea spray aerosol and particles in real-world emissions e.g.,.

If complex emissions from realistic sources are used, special consideration must be given to transmission efficiency from the emission source to the chamber. This is because losses can differ for various gas-phase compounds and particles, which may alter the air composition in an unaccounted way. Therefore, more complex inlet systems may be required than for the injection of individual compounds.

The diversity of chambers makes it often challenging to perform experiments in exactly the same way. One difference is, for example, the dilution rate, which leads to different process time scale while affecting the formation of products. This makes it more difficult to directly compare results. However, as the underlying atmospheric processes are the same, the findings (e.g. kinetic information) obtained after all chamber specific effects are adequately considered Sect. ), are expected to be the same. One key requirement is an appropriate experimental design ensuring that branching ratios of processes are similar, for example, that the fate of peroxy radicals in oxidation experiments is comparable (Sect. 6.2, Kenagy et al., 2024). One task for the further development of the ACTRIS chambers will be to demonstrate the comparability of results .

Recently a comparison of chamber performance was conducted between IASC and SAPHIR based on set of typical day- and night-time standard oxidation experiments using the same key instrumentation on both chambers . Exercises like this help to develop standards in the characterization of the most critical chamber parameters to be able to generalize chamber results and to harmonize the comparability of chamber data.

However, the diversity of the type of studies that can be performed in the different chambers and the specialization and availability of different instrumental approaches is also important to be recognized and fostered, because different aspects of the same atmospheric processes can then be studied in different chambers. A good degree of complementarity is an advantage to obtain a more comprehensive picture of the atmospheric chemistry involved in the processes.

Experiments carried out in the simulation chambers typically include many measurements required to derive meaningful results. Some of the instruments are serviced by the 3 ACTRIS Topical Centres for in situ observations . The centre for gas-phase species (Centre for Trace Gases in situ Measurements, CiGAS) is responsible for the measurements of selected volatile organic compounds (VOCs) using Proton-Transfer-Reaction Mass Spectrometry (PTR-MS), Chemical Ionisation Mass Spectrometry (CIMS) and Gas Chromatography (GC), as well as for instruments measuring nitrogen oxides (NO and NO2). The centre for aerosol in situ properties (Center for Aerosol In Situ - European Center for Aerosol Calibration and Characterization, CAIS-ECAC) ensures the data quality of particle number concentrations and size distributions, their chemical composition and optical properties and the number concentration of cloud condensation nuclei. Only few chambers are equipped to carry out cloud experiments. Instruments for this type of experiment are serviced by the centre for cloud properties (Centre for Cloud In Situ, CIS). All measurements that fully comply with the requirements of the calibration centres are marked in the data sets of the experiments.

The operators of the chamber facility are responsible for maintaining and documenting the quality assurance and control protocols and for providing the respective documentation for all other measurements. This includes calibration procedures that are performed regularly using certified calibration standards, calibration devices or other approaches developed by the experts at the chamber facility, for example for short-lived radicals.

The facility experts are responsible for processing of data to provide quality assured results (level 2 data, Fig. ) that are made available through the data centre. The reproducibility of the results must be documented to ensure that data is compliant with the FAIR principles. Results of chamber experiments may also include advanced data products (level 3 data, Sect. ).

Quality assurance for ACTRIS chamber experiments also includes the documentation of the exact timing of all actions during the experiment. This metadata is submitted to the ACTRIS data centre along with the experimental data. In the future, ACTRIS chambers will require an electronic form of documentation for a harmonised, machine-readable protocol.

ACTRIS simulation chambers provide access for the atmospheric research community, other transdisciplinary research communities, and users from industry. Access to the chambers is managed centrally by the ACTRIS Service Access Management Unit (SAMU) of the ACTRIS Head Office via an online application management system (Fig. ). Calls for applications related to specific topics may be issued from time to time, with funding provided to support travel to the facilities.

The application for access includes information about the users, a description of the experimental work programme and plans for the dissemination of the results. The feasibility of the work programme is checked by the experts at the chamber facilities, who may recommend adapting the plan or even suggest using a different chamber, which is better suited to the purpose of the experiments. Only after feasibility has been confirmed, access proposals that receive funding through competitive calls are reviewed by experts. In addition to scientific excellence, technical innovation and training are important evaluation criteria.

Access to a chamber facility allows the user(s) to define their own experiments and objectives with an individual experimental schedule. It is also possible to use several chambers to take advantage of the complementary nature of the different types of chambers as demonstrated in the study by . Data from the experiments can be used exclusively by the user(s) until the data is submitted to the data centre no later than 12 months after the access. Confidential agreements are only acceptable in cases where public access to the data is restricted, for example, if required by industry (Sect. ).

Physical presence at the chamber during the access period facilitates adjustments of the experiment plan based on the preliminary results. It moreover enables effective communication and collaboration between the users and the chamber providers. Interactions between users and chamber experts can also be fully or partly realised via remote involvement of the users. Remote access has the advantage of eliminating travel costs and reducing the carbon footprint of the access. In addition, there is generally more flexibility in the planning and timing of the experiments.

The purpose of the access may also focus on or include training, particularly for young researchers. This may be hands-on training in carrying out chamber experiments, and/or the operation of instruments or getting to know a certain type of detection technology. In addition, workshops and seminars provide user training in atmospheric chemistry, data evaluation, or modelling of chamber experiments.

The ACTRIS Data Centre provides free and open access to data from all ACTRIS National Facilities including atmospheric simulation chambers. The ACTRIS Data Centre implements FAIR (findability, accessibility, interoperability, and reusability) principles, which for example adds a digital object identifier (doi) to each dataset. The atmospheric simulation chamber data centre (https://data.eurochamp.org/, last access: 15 February 2026) was established in 2005 during the first EUROCHAMP project and has recently become the Atmospheric Simulation Chamber (ASC) unit of the ACTRIS Data Centre (https://data.actris.eu/, last access: 15 February 2026), from which all data can be accessed. This website provides an interface for specific search queries and data from simulation chambers is made available via three databases, offering access to the different types of data described below.

The Database of Atmospheric Simulation Chamber Studies (DASCS) this provides access to experimental and modelled time series from the experiments (defined as level 2 (L2) data). It is indicated whether the measurements are fully compliant with the regulations of one of the ACTRIS Topical Centres (Sect. ). The dataset includes metadata about the experiment (Sect. ).

Since its creation, the Atmospheric Simulation Chamber unit of the ACTRIS Data Centre has provided access to over 2000 chamber experiment datasets, over 500 infrared and mass spectra datasets, and over 330 advanced product datasets via the three databases (DASCS, LAR and LADP).

Chamber experiments are ideal for testing the performance of instruments under controlled realistic but also extreme conditions . One method is to generate standard samples for offline instruments . Chambers which can be operated at low pressure can help testing the performances of aircraft instrumentation at mid-to-high tropospheric conditions . Large chambers such as AIDA, EUPHORE, SAPHIR and HELIOS allow many instruments to be involved in comparison exercises e.g.,. Instrument comparisons in chambers are preferable to those conducted in the field, because the chambers ensure that all instruments sample the same air and the conditions can be systematically varied. This allows to identify potential dependencies of the instrument sensitivity and cross-sensitivities, and calibration or correction factors can be determined and verified. Instrument comparisons are part of the quality control procedures of instruments from observational facilities, which are serviced by the ACTRIS Topical Centres, or from other air quality monitoring stations . Instruments tests can also include future scenarios of atmospheric composition that may differ from current conditions as for example ammonia concentrations may increase due to its future use in energy production in a hydrogen-based economy .

One example of a successful collaboration between small to medium enterprises (SMEs), ACTRIS Topical Centres and chambers was the testing of a new generation of instruments for the measurement of NO2 using the ICAD Iterative Cavity-Enhanced Differential Optical Absorption Spectroscopy, Airyx, method, which is based on incoherent broadband cavity enhanced absorption spectroscopy (IBBCEAS) . These instruments could replace today's standard chemiluminescence instruments. The ICAD instrument was tested in many experiments in the SAPHIR chamber during 2022 including a comparison with ACTRIS standard instruments. Dedicated tests were carried out in 2023, when the ACTRIS Topical Centre CiGAS brought together NO2 instruments from 9 ACTRIS observational facilities. The comparison showed that measurements produced by the ICAD instrument had a high precision and accuracy and were less affected by interferences than chemiluminescence instruments using a molybdenum converter for NO2 detection.

Another example of a successful collaboration between small companies, simulation chambers, and observational sites was the validation of a newly developed aethalometer with an extended wavelength range and a total carbon analyser. The validation included tests at the EUPHORE chamber in 2022, followed by subsequent campaigns at several ACTRIS observational sites in 2023/2024.

Another example of a successful collaboration with SMEs is the development of the Portable Ice Nucleation Experiment PINE . This development was based on the experience of operating the AIDA chamber as expansion-type cloud simulation chamber and was conducted in close collaboration with the Bilfinger Nuclear & Energy Transition GmbH and the University of Leeds. The PINE chamber is the first mobile and commercially available instrument for the long-term 24/7 monitoring of ice-nucleating particle (INP) concentrations. It is also selected as a standard and reference instrument for INP measurements within the ACTRIS-CIS Topical Centre.

Low-cost sensors have the potential to complement sensor networks of standard reference instruments as the density of measurements can be significantly increased by deploying many sensors e.g.,. They are playing an increasing role in air quality monitoring, especially in urban areas, as they can supplement regional air quality monitoring networks . In addition, these sensors can be easily deployed on small unmanned aerial vehicles (drones) enabling horizontal and vertical exploration of trace gas concentrations at rather low costs. However, the stability of the calibration, the limit of detection, and the selectivity of especially electrochemical sensors are still challenging. Chamber experiments can support the calibration and characterization of these sensors to develop data evaluation strategies and standard operational procedures .

Nitrate (NO3-) CIMS instruments are widely used to measure condensable vapours with various instrumental specifics such as inlet designs and ionization methods. To ensure comparability of measurements, a unified inlet system was designed together with the ACTRIS CiGAS Topical Centre. In a set of chamber experiments at the ACD-C facility, the detection of sulfuric acid by several instruments was compared using commonly applied calibration setups. Furthermore, the instrument responses to oxidized organic compounds from α-pinene oxidation and organic standards were investigated under different experimental conditions. First results show that observed mixing ratios as well as the response of the instruments to the changes of the chamber conditions differed, which underlines the importance of instrument comparison efforts and standardised measurement procedures.

Another example for validating an offline instrument in chamber experiments was the development and test of a broadband light analyser of complex aerosol (BLAnCA), which provides measurements of the aerosol absorption coefficient with high spectral resolution . The spectrometer and light source have an unprecedented broad spectral range, enabling measurements to be taken with 5 nm resolution in the spectral range from 375–1000 nm. This resolution allows to distinguish spectral absorption features for a wide variety of aerosols sampled on filters, such those of black and brown carbon, mineral dust aerosols .

Parameters that determine chemical mechanisms and reaction kinetics data are key for air quality models. Consequently, the validity and completeness of these parameters largely control the accuracy of air quality predictions. Well designed and conducted chamber experiments can provide these data with high accuracy. For example, the temperature dependence of rate coefficient for VOC reactions with different oxidants can be studied over a wide range in chambers in which the temperature can be controlled e.g.,.

Depending on the chemical complexity of the experiment, specific reaction pathways can be studied . Often entire reaction systems consisting of competing reaction pathways are studied under atmospheric conditions. The results are often interpreted using chemical box models, whose development is an integral part of research using chambers e.g.,. These models allow access to a deeper level of insight regarding the roles and concentrations of intermediate species that often cannot be accessed directly by measurements. This can also be combined with theoretical studies e.g.,. Chamber experiments can involve either a single precursor reactive organic species , or a mixture of them e.g.,.

One focus of chamber studies is on the fate of peroxy radicals (RO2) formed in the oxidation of VOCs, which strongly depends on the availability of nitric oxide (NO). Specifically in environments with high concentrations of VOC and O3, chemical mechanisms often fail to reproduce observations in the field . Chamber experiments can be used to simulate these conditions, but careful planning of experiments is required to ensure that the fate of RO2 radicals is realistic. If high VOC and oxidant concentrations are used, as is commonly the case in experiments on SOA, the fate of RO2 radicals can be dominated by RO2 + RO2 radical reactions, which are typically less important in the atmosphere than other RO2 reactions .

Conditions, where loss rates of RO2 radicals in the reaction with NO become small, can give rise to other reaction pathways such as unimolecular RO2 reactions, and lead to other product species . Consecutive RO2 isomerisation reactions are also the central step in the formation of highly oxygenated organic molecules (HOM), which have been recognised to drive rapid SOA formation e.g.,. Another important type of RO2 reactions are reactions with other radicals such as RO2, HO2 and OH. Recent advances in mass spectrometry enables the detection of ester products from the self- and cross-reaction of peroxy radicals and the formation of dimer species . Such accretion products are believed to play a major role in SOA formation, but the set of experimental data to support this hypothesis is still sparse. A recent review also highlights significant knowledge gaps in RO2 + HO2 reactions .

Examples of studies providing refined kinetic data are experiments on the oxidation of isoprene by the OH radical, where evidence for an enhanced OH regeneration from RO2 isomerisation reactions was found . Other examples are the discovery of previously unrecognised reaction pathways in the oxidation of isoprene by the NO3 radical, leading to the formation of nitrate epoxide products and the elucidation of the role of aromatic photo-oxidation in affecting atmospheric acidity . Criegee intermediates, formed in ozonolysis reactions, have also been studied experimentally as well as by theory e.g.,. Their chemistry remains an important area of research in chambers owing to the complex product distributions observed recently in ozonolysis reactions e.g., affecting also particle formation. have established a new relative rate technique that can be used to determine the reaction kinetics of Criegee intermediates. This method enables studies under atmospheric conditions, and measurements of co-reactants with much lower volatility than was previously possible. have performed extensive gas- and condensed-phase studies on a key plant hormone, methyl salicylate. This study highlights the many different interactions that need to be considered when studying the atmospheric interactions of semi-volatile biospheric emissions within ecosystems.

Simulation chambers enhance our understanding of aerosol particles, which are an elusive yet significant component of the Earth's atmosphere that has a strong impact on health, climate, atmospheric chemistry and the environment. Experiments provide insights into the mechanisms of their formation and transformation during ageing, as well as on their physicochemical, optical and hygroscopic properties. This allows to develop predictive relationships that improve the representation of aerosols in climate models and satellite retrieval algorithms. Various types of aerosols have been studied in ACTRIS chambers. Some examples are discussed below.

Experiments on SOA examine the interactions of various VOC precursors from both biogenic sources e.g. and anthropogenic sources e.g., as well as their mixtures e.g.. For example, studies have demonstrated that aerosol yields can be significantly reduced by increasing the relative importance of the oxidation of VOCs with low aerosol yields OH scavenging,, and by shifting the reaction of short-lived intermediate peroxy radicals to pathways with low aerosol yields product scavenging,. Other studies have shown the importance of considering both relative humidity and temperature for SOA formation and aerosol properties .

Controlled chamber experiments under various plume-like conditions are well suited to making robust predictions about the air quality associated with wildfire events, many of which are expected to increase in number and intensity in the future, resulting in substantial particle emissions and SOA formation . However, as the chemistry within wildfire plumes is highly dynamic and occurs under extreme conditions, current chemical mechanisms often cannot reliably predict the formation of secondary pollutants .

Simulation chambers have also been increasingly used to determine the precise physicochemical properties of aerosols, such as viscosity, optical and hygroscopic properties. For instance, showed that the viscosity of SOA from the ozonolysis of α-pinene changes from a predominantly glassy state to a predominantly liquid state and reported changes in the viscosity of α-pinene ozonolysis SOA induced by temperature changes. measured the spectral complex refractive index of mineral dust in the visible and infrared spectrum. illustrated, for the first time, that Icelandic dust is mineralogically distinct and exhibits greater light absorption than mid-latitude dust. demonstrated that mineral dust can act as a sink for gas-phase glyoxal at high relative humidity, forming oligomers. This suggests that dust aerosols could play a significant role in the formation of organic aerosol.

The hygroscopic and ice-nucleating properties of soot and mineral dust have also been studied. They contribute to the formation of contrails, which also has an impact on the climate . Other studies investigated the physical and chemical modification of aerosol properties during simulated long-range transport or by cloud and ice formation processes .

Significant efforts have been made to develop well-characterised, versatile methodologies and tools for chamber studies on aerosols, allowing the diversity of natural aerosol particles to be reproduced. For instance, demonstrated how to produce soot from a propane flame generator for studying its optical properties in the CESAM chamber. For studying optical properties of brown carbon, SOA can be generated from the oxidation of biomass burning products (e.g., catechol and guaiacol oxidation) or multiphase chemistry involving fog chemistry . Recently, a generator has been developed and characterised that produces poly-dispersed airborne aerosol particles from native soils collected across global deserts . Methods to produce sea spray particles using bubble-mediated aerosol have also been developed.

Another focus of atmospheric chemistry studies in simulation chambers is the quantification and characterisation of emissions. Chambers are used to study realistic emissions from anthropogenic sources, such as exhaust from combustion engines , biomass burning , candle burning , emissions from biogenic sources such as trees and their chemical transformations during ageing. These emissions can be transferred into the chamber for further oxidation to study their chemical transformation. Some chambers study the interaction between gas-phase species and various types of surfaces , including ice surfaces . For example, the study of heterogeneous reactions on particles and the exchange of substances between the gas and particle phases are examined .

In addition to the monitoring of trace gases and measurements of the physical and chemical properties of particles, the identification of the sources is also an important part of the remit of many ACTRIS observational facilities. This is often achieved by positive matrix factorization, in which the time series of fingerprint patterns can be attributed to specific sources like traffic, industry or oxidation processes e.g.,. Chamber experiments can be used to identify specific patterns of various aerosol sources by injecting different types of aerosols or gas mixtures for secondary aerosol formation and applying similar methods . One additional benefit of chamber experiments is the controlled initial conditions, and the availability of cutting-edge instruments such as high-resolution mass spectrometers which are typically not employed during routine measurements at observational facilities. Chamber experiments with ambient air also contributed to the identification of emission sources .

Chamber experiments can also help connecting observations of gas-phase species and particles to their health effects. Some chambers (EUPHORE, CESAM, PACS, KASC) have established systems to expose cells or even animals to well-defined mixtures of pollutants that are directly taken from sources or are chemically aged in the chamber. In addition, selected chambers such as MAC have the capability to conduct controlled human exposure studies under clinical trial conditions, allowing direct assessment of human responses to realistic, well-characterised pollutant mixtures . In this context, the oxidative potential of particles, used as a proxy for aerosol-induced oxidative stress and recommended for enhanced monitoring under the EU Ambient Air Quality Directive (EU) 2024/2881, can be evaluated in simulation chambers and related to the particle composition, their source and ageing state. By systematic cell exposure studies, a parametrisation can be developed that will help predicting the health effect using parameters observable at the ACTRIS facilities. Similarly, the ChAMBRe chamber was used to simulate real-world summer and winter aerosol pollution scenarios, in which aerosol on filters were assessed for their oxidative potential and toxicological properties . Another application of simulation chambers is the investigation of transmission pathways of pathogens. Representative aerosol mixtures can be created to determine the transmission efficiency of pathogens under realistic conditions as well as to characterize possible pathogen abatement strategies .

The effect of potential air pollution strategies can be evaluated in chambers. Experiments in the ChAMBRe chamber, for example, were used to assess the ability of different plants to remove NO2, black carbon, and dust and the effect of the removal on the viability of airborne bacteria . Emission control strategies, structural changes in the energy production and socio-economic trends are all expected to alter future anthropogenic emissions. The changing climate will also affect emissions from the biosphere. These will dynamically change future atmospheric chemical conditions, and while some changes are expected to improve air quality, there may also be unexpected effects from the emission of new species. Such future scenarios can be simulated in chamber experiments to assess emerging and future air quality impacts. Examples are:

The increasingly strict legislation for vehicular emissions (e.g., Euro standards) has led to continuously decreasing NOx concentrations in cities. As a result, the changing fate of peroxy radicals induces significant changes in the composition of urban air, including the formation of secondary pollutants. In a wider context, new air quality regulations and mitigation policies might change pollution scenarios, with new emerging contaminants and VOC/NOx ratios that can be assessed in chambers.

Data from chamber experiments include various high-level data (Sect. ) that can be used as reference for the data evaluation of ACTRIS observations as well as for satellite algorithm initiation and data interpretation. Optical properties of particles are one example. ACTRIS observations include monitoring of particles in the atmosphere by in situ and remote sensing. Chamber experiments can provide information of absorption and scattering properties of chemically and physically well-characterised particles using cutting-edge instruments. Particle mixtures that are representative for the atmosphere can be produced in chamber experiments in oxidation experiments and/or they are transferred into the chamber using realistic sources (Sect. ).

Similarly, reference optical absorption spectra and mass spectra of atmospheric constituents, from single species to complex mixtures can be determined in chamber experiments. This helps the interpretation of field measurements at observational sites, where the complexity of ambient air makes the unambiguous species identification often challenging. For example, show that the use of infrared optical properties derived from experiments in the CESAM chamber for mineral dust from different source regions were efficient in reconciling downward surface irradiance over the Lampedusa ACTRIS site with longwave irradiance measured on board an overpassing aircraft . In contrast, more generic parametrisations from the OPAC database performed less well.