AMT Atmospheric Measurement Techniques AMT Atmos. Meas. Tech. 1867-8548 Copernicus Publications Göttingen, Germany 10.5194/amt-6-1647-2013 Development and testing of an online method to measure ambient fine particulate reactive oxygen species (ROS) based on the 2',7'-dichlorofluorescin (DCFH) assay King L. E. 1 Weber R. J. https://orcid.org/0000-0003-0765-8035 1 School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA, USA 11 07 2013 6 7 1647 1658 Copyright: © 2013 L. E. King 2013 This work is licensed under the Creative Commons Attribution 3.0 Unported License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/3.0/ This article is available from https://amt.copernicus.org/articles/6/1647/2013/amt-6-1647-2013.html The full text article is available as a PDF file from https://amt.copernicus.org/articles/6/1647/2013/amt-6-1647-2013.pdf

An online, semi-continuous instrument to measure fine particle (PM<sub>2.5</sub>) reactive oxygen species (ROS) was developed based on the fluorescent probe 2'7'-dichlorofluorescin (DCFH). Parameters that influence probe response were first characterized to develop an optimal method for use in a field instrument. The online method used a mist chamber scrubber to collect total (gas plus particle) ROS components (ROS<sub>t</sub>) alternating with gas phase ROS (ROS<sub>g</sub>) by means of an inline filter. Particle phase ROS (ROS<sub>p</sub>) was determined by the difference between ROS<sub>t</sub> and ROS<sub>g</sub>. The instrument was deployed in urban Atlanta, Georgia, USA, and at a rural site during various seasons. Concentrations from the online instrument generally agreed well with those from an intensive filter measurement of ROS<sub>p</sub>. Concentrations of the ROS<sub>p</sub> measurements made with this instrument were lower than reported in other studies, often below the instrument's average limit of detection (0.15 nmol H<sub>2</sub>O<sub>2</sub> equivalents m<sup>−3</sup>). Mean ROS<sub>p</sub> concentrations were 0.26 nmol H<sub>2</sub>O<sub>2</sub> equivalents m<sup>−3</sup> at the Atlanta urban sites compared to 0.14 nmol H<sub>2</sub>O<sub>2</sub> equivalents m<sup>−3</sup> at the rural site.

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