Preprints
https://doi.org/10.5194/amt-2020-490
https://doi.org/10.5194/amt-2020-490

  08 Jan 2021

08 Jan 2021

Review status: this preprint is currently under review for the journal AMT.

Comparative characterization of bio-aerosol nebulizers in connection to atmospheric simulation chambers

Silvia G. Danelli1,2, Marco Brunoldi1,2, Dario Massabò1,2, Franco Parodi2, Virginia Vernocchi1,2, and Paolo Prati1,2 Silvia G. Danelli et al.
  • 1Dipartimento di Fisica - Università di Genova, via Dodecaneso 33, 5 16146, Genova (IT)
  • 2INFN – Sezione di Genova, via Dodecaneso 33, 16146, Genova (IT)

Abstract. The interplay of bio-aerosol dispersion and impact, meteorology, air quality is gaining increasing interest in the wide spectrum of atmospheric science. Experiments conducted inside confined artificial environments, such as the Atmospheric Simulations Chambers (ASCs), where atmospheric conditions and composition are controlled, can provide valuable information on bio-aerosol viability, dispersion, and impact. We focus here on the reproducible aerosolization and injection of viable microorganisms into an ASC, the first and crucial step of any experimental protocol to expose bio-aerosol at different atmospheric conditions. We compare the performance of three nebulizers specifically designed for bioaerosol applications: the Collison nebulizer, the Blaustein Atomizing Modules (BLAM) and the Sparging Liquid Aerosol Generator (SLAG), all manufactured and commercialized by CH TECHNOLOGIES. The comparison refers to operating conditions and the concentration of viable bacteria at the nebulizer outlet, with the final goal to measure the reproducibility of the nebulization procedure and assess the application in experiments at ASCs. A typical bacterial test model, Escherichia coli (ATCC® 25922™), was selected for such characterization. Bacteria suspensions, with a concentration around 108 CFU ml−1, were first aerosolized at different air pressures and collected by a Liquid Impinger, to obtain a correlation curve between airflow and nebulized bacteria, for each generator. Afterwards, bacteria were aerosolized inside the atmospheric simulation chamber ChAMBRe (Chamber for Aerosol Modelling and Bio-aerosol Research) to measure the reproducibility of the whole procedure. An overall reproducibility of 11 % was obtained with each nebulizer through a set of baseline experiments.

Silvia G. Danelli et al.

Status: open (until 30 Apr 2021)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on amt-2020-490', Anonymous Referee #1, 13 Feb 2021 reply
    • CC1: 'Reply on RC1', Silvia Giulia Danelli, 16 Feb 2021 reply
  • RC2: 'Comment on amt-2020-490', Michel Attoui, 12 Apr 2021 reply
  • RC3: 'Comment on amt-2020-490', Jing Wang, 12 Apr 2021 reply

Silvia G. Danelli et al.

Silvia G. Danelli et al.

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