Preprints
https://doi.org/10.5194/amt-2021-82
https://doi.org/10.5194/amt-2021-82

  22 Mar 2021

22 Mar 2021

Review status: this preprint is currently under review for the journal AMT.

An automated system for trace gas flux measurements from plantfoliage and other plant compartments

Lukas Kohl1,2,, Markku Koskinen1,2,, Tatu Polvinen1,2, Salla Tenhovirta1,2, Kaisa Rissanen3, Marjo Patama1,2, Alessandro Zanetti4,2, and Mari Pihlatie1,2,5 Lukas Kohl et al.
  • 1University of Helsinki, Department of Agriculture, Environmental Soil Science Unit, Viikinkaari 9, 00790 Helsinki, Finland
  • 2University of Helsinki, Institute for Atmosphere and Earth System Research/Forest Research, Viikinkaari 9, 00790 Helsinki, Finland
  • 3Département des sciences biologiques, Université du Québec à Montréal, 141 Avenue du Président-Kennedy, Montreal, QC H2X 1Y4, Canada
  • 4University of Helsinki, Department of Forestry, Ladokartanonkaari 7, 00790 Helsinki, Finland
  • 5University of Helsinki Viikki Plant Science Center (VIPS), Viikinkaari 9, 00790 Helsinki, Finland
  • These authors contributed equally to this work.

Abstract. Plant shoots can act as sources or sinks of trace gases including methane and nitrous oxide. Accurate measurementsof these trace gas fluxes require enclosing of shoots in closed non-steady state chambers. Due to plant physiological activity, this type of enclosures, however, lead to CO2 depletion in the enclosed air volume, condensation of transpired water, and warmingof the enclosures exposed to sunlight, all of which may bias the flux measurements. Here, we present PlasTraGAS, ab novel measurement system designed for continuous and automated measurements of trace gas and volatile organic compound (VOC) fluxes from plant shoots. The system uses transparent shoot enclosures equipped with Peltier cooling elements and automatically replaces fixated CO2 and removes transpired water from the enclosure. The system is designed for measuring trace gasfluxes over extended periods, capturing diurnal and seasonal variations and linking trace gas exchange to plant physiologicalfunctioning and environmental drivers. Initial measurements show daytime CH4 emissions two pine shoots of 0.056 and 0.089 nmol g−1 foliage d.w.h−1or 7.80 and 13.1 nmol m−2 h−1. Simultaneously measured CO2 uptake rates were 9.2 and 7.6 mmol m−2 sec−1 and transpiration rates of 1.24 and 0.90 mol m−2 h−1. Concurrent measurement of VOC emissionsdemonstrated that potential effects of spectral interferences on CH4 flux measurements were at least ten-fold smaller than themeasured CH4 fluxes. Overall, this new system solves multiple technical problems that so far prevented automated plant shoottrace gas flux measurements, and holds the potential for providing important new insights into the role of plant foliage in the global CH4 and N2O cycles.

Lukas Kohl et al.

Status: open (until 17 May 2021)

Comment types: AC – author | RC – referee | CC – community | EC – editor | CEC – chief editor | : Report abuse
  • RC1: 'Comment on amt-2021-82', Anonymous Referee #1, 04 Apr 2021 reply

Lukas Kohl et al.

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An automated system for trace gas flux measurements from plant foliage and other plant compartments - raw data and data processing code Lukas Kohl, Markku Koskinen , Tatu Polvinen, Salla Tenhovirta, Kaisa Rissanen, Marjo Patama, Alessandro Zanetti, and Mari Pihlatie https://doi.org/10.5281/zenodo.4609836

Lukas Kohl et al.

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Short summary
We present PlasTraGAS, a measurement system designed for continuous and automated measurements of trace gas and volatile organic compound (VOC) fluxes from plant shoots. PlaSTraGAS uses transparent shoot enclosures equipped with cooling elements,  automatically replaces fixated CO2, and removes transpired water from the enclosure, thus solving multiple technical problems that so far prevented automated plant shoot trace gas flux measurements.