Articles | Volume 11, issue 11
https://doi.org/10.5194/amt-11-6327-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/amt-11-6327-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
23 Nov 2018
Research article |
| 23 Nov 2018
| 23 Nov 2018
Twin-plate Ice Nucleation Assay (TINA) with infrared detection for high-throughput droplet freezing experiments with biological ice nuclei in laboratory and field samples
Anna T. Kunert
Multiphase Chemistry Department, Max Planck Institute for Chemistry,
55128 Mainz, Germany
Mark Lamneck
Instrument Development and Electronics, Max Planck Institute for
Chemistry, 55128 Mainz, Germany
Frank Helleis
Instrument Development and Electronics, Max Planck Institute for
Chemistry, 55128 Mainz, Germany
Ulrich Pöschl
Multiphase Chemistry Department, Max Planck Institute for Chemistry,
55128 Mainz, Germany
Mira L. Pöhlker
CORRESPONDING AUTHOR
Multiphase Chemistry Department, Max Planck Institute for Chemistry,
55128 Mainz, Germany
Janine Fröhlich-Nowoisky
CORRESPONDING AUTHOR
Multiphase Chemistry Department, Max Planck Institute for Chemistry,
55128 Mainz, Germany
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Cited
46 citations as recorded by crossref.
- Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges M. Tarn et al. https://doi.org/10.1063/5.0236911
- Bioaerosols and atmospheric ice nuclei in a Mediterranean dryland: community changes related to rainfall K. Tang et al. https://doi.org/10.5194/bg-19-71-2022
- Peltigera lichen thalli produce highly potent ice-nucleating agents R. Eufemio et al. https://doi.org/10.5194/bg-22-2087-2025
- Cellulose Nanofibers Impart Melt Resistance to Ice through Optical and Thermal Mechanisms E. Asenath-Smith et al. https://doi.org/10.1021/acs.jpcc.4c07592
- Overview of biological ice nucleating particles in the atmosphere S. Huang et al. https://doi.org/10.1016/j.envint.2020.106197
- Pragmatic protocols for working cleanly when measuring ice nucleating particles K. Barry et al. https://doi.org/10.1016/j.atmosres.2020.105419
- A Microfluidic Device for Automated High Throughput Detection of Ice Nucleation of Snomax® P. Roy et al. https://doi.org/10.3390/mi12030296
- Ice Nucleating Activity and Residual Particle Morphology of Bulk Seawater and Sea Surface Microlayer P. Roy et al. https://doi.org/10.1021/acsearthspacechem.1c00175
- Lichen species across Alaska produce highly active and stable ice nucleators R. Eufemio et al. https://doi.org/10.5194/bg-20-2805-2023
- Airborne bacterial communities over the Tibetan and Mongolian Plateaus: variations and their possible sources J. Qi et al. https://doi.org/10.1016/j.atmosres.2020.105215
- Ice Nucleation Activity of Alpine Bioaerosol Emitted in Vicinity of a Birch Forest T. Seifried et al. https://doi.org/10.3390/atmos12060779
- A Drone-Based Bioaerosol Sampling System to Monitor Ice Nucleation Particles in the Lower Atmosphere P. Bieber et al. https://doi.org/10.3390/rs12030552
- Micro-thermography for imaging ice crystal growth and nucleation inside non-transparent materials M. Zalazar et al. https://doi.org/10.1063/5.0142245
- Ice Nucleation Activity of Perfluorinated Organic Acids R. Schwidetzky et al. https://doi.org/10.1021/acs.jpclett.1c00604
- Interfacial Water Ordering Is Insufficient to Explain Ice-Nucleating Protein Activity M. Lukas et al. https://doi.org/10.1021/acs.jpclett.0c03163
- HUB: a method to model and extract the distribution of ice nucleation temperatures from drop-freezing experiments I. de Almeida Ribeiro et al. https://doi.org/10.5194/acp-23-5623-2023
- Evaluation of different sampling methods to determine the ice-nucleating particle concentration in the atmosphere using the GRAnada Ice Nuclei Spectrometer (GRAINS) E. Bazo et al. https://doi.org/10.5194/amt-19-2695-2026
- Biochemical characterization of a fungal ice nucleation protein from Podila clonocystis B. Walsh et al. https://doi.org/10.1038/s41598-026-49881-6
- Ice nucleating behavior of different tree pollen in the immersion mode E. Gute & J. Abbatt https://doi.org/10.1016/j.atmosenv.2020.117488
- Do Inhibition Studies of Ice Crystallization Indicate Cryoprotectant Efficacy? P. Fernando et al. https://doi.org/10.1021/acs.cgd.5c00221
- Inhibition of Bacterial Ice Nucleators Is Not an Intrinsic Property of Antifreeze Proteins R. Schwidetzky et al. https://doi.org/10.1021/acs.jpcb.0c03001
- Determination of the protein content of complex samples by aromatic amino acid analysis, liquid chromatography-UV absorbance, and colorimetry K. Reinmuth-Selzle et al. https://doi.org/10.1007/s00216-022-03910-1
- Membranes Are Decisive for Maximum Freezing Efficiency of Bacterial Ice Nucleators R. Schwidetzky et al. https://doi.org/10.1021/acs.jpclett.1c03118
- Polyol-Induced 100-Fold Enhancement of Bacterial Ice Nucleation Efficiency G. Renzer et al. https://doi.org/10.1021/acs.jpcc.4c07422
- Hierarchical assembly and environmental enhancement of bacterial ice nucleators G. Renzer et al. https://doi.org/10.1073/pnas.2409283121
- Droplet freezing assays using a nanoliter osmometer J. Lee et al. https://doi.org/10.1016/j.cryobiol.2023.104584
- Macromolecular fungal ice nuclei in Fusarium: effects of physical and chemical processing A. Kunert et al. https://doi.org/10.5194/bg-16-4647-2019
- Scots Pines (Pinus sylvestris) as Sources of Biological Ice-Nucleating Macromolecules (INMs) T. Seifried et al. https://doi.org/10.3390/atmos14020266
- An improved Freezing Ice Nucleation Detection Analyzer (FINDA) for droplet immersion freezing measurement K. Wang et al. https://doi.org/10.5194/amt-18-5823-2025
- Isolation of subpollen particles (SPPs) of birch: SPPs are potential carriers of ice nucleating macromolecules J. Burkart et al. https://doi.org/10.5194/bg-18-5751-2021
- Toward Understanding Bacterial Ice Nucleation M. Lukas et al. https://doi.org/10.1021/acs.jpcb.1c09342
- Electrostatic Interactions Control the Functionality of Bacterial Ice Nucleators M. Lukas et al. https://doi.org/10.1021/jacs.9b13069
- Specific Ion–Protein Interactions Influence Bacterial Ice Nucleation R. Schwidetzky et al. https://doi.org/10.1002/chem.202004630
- Measurement report: Ice nucleation ability of perthite feldspar powder J. Canet et al. https://doi.org/10.5194/acp-26-5635-2026
- Development of the drop Freezing Ice Nuclei Counter (FINC), intercomparison of droplet freezing techniques, and use of soluble lignin as an atmospheric ice nucleation standard A. Miller et al. https://doi.org/10.5194/amt-14-3131-2021
- The role of structural order in heterogeneous ice nucleation G. Sosso et al. https://doi.org/10.1039/D1SC06338C
- Micro-PINGUIN: microtiter-plate-based instrument for ice nucleation detection in gallium with an infrared camera C. Wieber et al. https://doi.org/10.5194/amt-17-2707-2024
- Aggregation of ice-nucleating macromolecules from Betula pendula pollen determines ice nucleation efficiency F. Wieland et al. https://doi.org/10.5194/bg-22-103-2025
- Advances in automated real‐time flow cytometry for monitoring of bioreactor processes A. Heins et al. https://doi.org/10.1002/elsc.202100082
- Impact of Perfluorinated Organic Acids on Bacterial Ice Nucleators G. Renzer et al. https://doi.org/10.1021/acs.jpcb.5c07036
- Functional aggregation of cell-free proteins enables fungal ice nucleation R. Schwidetzky et al. https://doi.org/10.1073/pnas.2303243120
- Design Principles for β-Solenoid Stability via Covalent and Electrostatic Capping Motifs R. Eufemio et al. https://doi.org/10.1021/acs.jpclett.6c01287
- A previously unrecognized class of fungal ice-nucleating proteins with bacterial ancestry R. Eufemio et al. https://doi.org/10.1126/sciadv.aed9652
- A pyroelectric thermal sensor for automated ice nucleation detection F. Cook et al. https://doi.org/10.5194/amt-13-2785-2020
- Chemical and physical equilibria shape dual ice-nucleation pathways in an organic crystal G. Renzer et al. https://doi.org/10.1038/s42004-026-02086-4
- The Microfluidic Ice Nuclei Counter Zürich (MINCZ): a platform for homogeneous and heterogeneous ice nucleation F. Isenrich et al. https://doi.org/10.5194/amt-15-5367-2022
46 citations as recorded by crossref.
- Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges M. Tarn et al. https://doi.org/10.1063/5.0236911
- Bioaerosols and atmospheric ice nuclei in a Mediterranean dryland: community changes related to rainfall K. Tang et al. https://doi.org/10.5194/bg-19-71-2022
- Peltigera lichen thalli produce highly potent ice-nucleating agents R. Eufemio et al. https://doi.org/10.5194/bg-22-2087-2025
- Cellulose Nanofibers Impart Melt Resistance to Ice through Optical and Thermal Mechanisms E. Asenath-Smith et al. https://doi.org/10.1021/acs.jpcc.4c07592
- Overview of biological ice nucleating particles in the atmosphere S. Huang et al. https://doi.org/10.1016/j.envint.2020.106197
- Pragmatic protocols for working cleanly when measuring ice nucleating particles K. Barry et al. https://doi.org/10.1016/j.atmosres.2020.105419
- A Microfluidic Device for Automated High Throughput Detection of Ice Nucleation of Snomax® P. Roy et al. https://doi.org/10.3390/mi12030296
- Ice Nucleating Activity and Residual Particle Morphology of Bulk Seawater and Sea Surface Microlayer P. Roy et al. https://doi.org/10.1021/acsearthspacechem.1c00175
- Lichen species across Alaska produce highly active and stable ice nucleators R. Eufemio et al. https://doi.org/10.5194/bg-20-2805-2023
- Airborne bacterial communities over the Tibetan and Mongolian Plateaus: variations and their possible sources J. Qi et al. https://doi.org/10.1016/j.atmosres.2020.105215
- Ice Nucleation Activity of Alpine Bioaerosol Emitted in Vicinity of a Birch Forest T. Seifried et al. https://doi.org/10.3390/atmos12060779
- A Drone-Based Bioaerosol Sampling System to Monitor Ice Nucleation Particles in the Lower Atmosphere P. Bieber et al. https://doi.org/10.3390/rs12030552
- Micro-thermography for imaging ice crystal growth and nucleation inside non-transparent materials M. Zalazar et al. https://doi.org/10.1063/5.0142245
- Ice Nucleation Activity of Perfluorinated Organic Acids R. Schwidetzky et al. https://doi.org/10.1021/acs.jpclett.1c00604
- Interfacial Water Ordering Is Insufficient to Explain Ice-Nucleating Protein Activity M. Lukas et al. https://doi.org/10.1021/acs.jpclett.0c03163
- HUB: a method to model and extract the distribution of ice nucleation temperatures from drop-freezing experiments I. de Almeida Ribeiro et al. https://doi.org/10.5194/acp-23-5623-2023
- Evaluation of different sampling methods to determine the ice-nucleating particle concentration in the atmosphere using the GRAnada Ice Nuclei Spectrometer (GRAINS) E. Bazo et al. https://doi.org/10.5194/amt-19-2695-2026
- Biochemical characterization of a fungal ice nucleation protein from Podila clonocystis B. Walsh et al. https://doi.org/10.1038/s41598-026-49881-6
- Ice nucleating behavior of different tree pollen in the immersion mode E. Gute & J. Abbatt https://doi.org/10.1016/j.atmosenv.2020.117488
- Do Inhibition Studies of Ice Crystallization Indicate Cryoprotectant Efficacy? P. Fernando et al. https://doi.org/10.1021/acs.cgd.5c00221
- Inhibition of Bacterial Ice Nucleators Is Not an Intrinsic Property of Antifreeze Proteins R. Schwidetzky et al. https://doi.org/10.1021/acs.jpcb.0c03001
- Determination of the protein content of complex samples by aromatic amino acid analysis, liquid chromatography-UV absorbance, and colorimetry K. Reinmuth-Selzle et al. https://doi.org/10.1007/s00216-022-03910-1
- Membranes Are Decisive for Maximum Freezing Efficiency of Bacterial Ice Nucleators R. Schwidetzky et al. https://doi.org/10.1021/acs.jpclett.1c03118
- Polyol-Induced 100-Fold Enhancement of Bacterial Ice Nucleation Efficiency G. Renzer et al. https://doi.org/10.1021/acs.jpcc.4c07422
- Hierarchical assembly and environmental enhancement of bacterial ice nucleators G. Renzer et al. https://doi.org/10.1073/pnas.2409283121
- Droplet freezing assays using a nanoliter osmometer J. Lee et al. https://doi.org/10.1016/j.cryobiol.2023.104584
- Macromolecular fungal ice nuclei in Fusarium: effects of physical and chemical processing A. Kunert et al. https://doi.org/10.5194/bg-16-4647-2019
- Scots Pines (Pinus sylvestris) as Sources of Biological Ice-Nucleating Macromolecules (INMs) T. Seifried et al. https://doi.org/10.3390/atmos14020266
- An improved Freezing Ice Nucleation Detection Analyzer (FINDA) for droplet immersion freezing measurement K. Wang et al. https://doi.org/10.5194/amt-18-5823-2025
- Isolation of subpollen particles (SPPs) of birch: SPPs are potential carriers of ice nucleating macromolecules J. Burkart et al. https://doi.org/10.5194/bg-18-5751-2021
- Toward Understanding Bacterial Ice Nucleation M. Lukas et al. https://doi.org/10.1021/acs.jpcb.1c09342
- Electrostatic Interactions Control the Functionality of Bacterial Ice Nucleators M. Lukas et al. https://doi.org/10.1021/jacs.9b13069
- Specific Ion–Protein Interactions Influence Bacterial Ice Nucleation R. Schwidetzky et al. https://doi.org/10.1002/chem.202004630
- Measurement report: Ice nucleation ability of perthite feldspar powder J. Canet et al. https://doi.org/10.5194/acp-26-5635-2026
- Development of the drop Freezing Ice Nuclei Counter (FINC), intercomparison of droplet freezing techniques, and use of soluble lignin as an atmospheric ice nucleation standard A. Miller et al. https://doi.org/10.5194/amt-14-3131-2021
- The role of structural order in heterogeneous ice nucleation G. Sosso et al. https://doi.org/10.1039/D1SC06338C
- Micro-PINGUIN: microtiter-plate-based instrument for ice nucleation detection in gallium with an infrared camera C. Wieber et al. https://doi.org/10.5194/amt-17-2707-2024
- Aggregation of ice-nucleating macromolecules from Betula pendula pollen determines ice nucleation efficiency F. Wieland et al. https://doi.org/10.5194/bg-22-103-2025
- Advances in automated real‐time flow cytometry for monitoring of bioreactor processes A. Heins et al. https://doi.org/10.1002/elsc.202100082
- Impact of Perfluorinated Organic Acids on Bacterial Ice Nucleators G. Renzer et al. https://doi.org/10.1021/acs.jpcb.5c07036
- Functional aggregation of cell-free proteins enables fungal ice nucleation R. Schwidetzky et al. https://doi.org/10.1073/pnas.2303243120
- Design Principles for β-Solenoid Stability via Covalent and Electrostatic Capping Motifs R. Eufemio et al. https://doi.org/10.1021/acs.jpclett.6c01287
- A previously unrecognized class of fungal ice-nucleating proteins with bacterial ancestry R. Eufemio et al. https://doi.org/10.1126/sciadv.aed9652
- A pyroelectric thermal sensor for automated ice nucleation detection F. Cook et al. https://doi.org/10.5194/amt-13-2785-2020
- Chemical and physical equilibria shape dual ice-nucleation pathways in an organic crystal G. Renzer et al. https://doi.org/10.1038/s42004-026-02086-4
- The Microfluidic Ice Nuclei Counter Zürich (MINCZ): a platform for homogeneous and heterogeneous ice nucleation F. Isenrich et al. https://doi.org/10.5194/amt-15-5367-2022
Saved (final revised paper)
Latest update: 02 Sep 2026
Short summary
The new Twin-plate Ice Nucleation Assay with infrared detection for high-throughput droplet freezing experiments in microliter-sized droplets is introduced, which was tested and characterized with bacterial and fungal ice nuclei. It was applied to investigate the influence of chemical processing on the activity of biological ice nuclei, and aqueous extracts of atmospheric aerosols were studied for ice nuclei activity.
The new Twin-plate Ice Nucleation Assay with infrared detection for high-throughput droplet...
