This study explores nitrous oxide (N₂O) fluxes from a nutrient-poor sub-Arctic peatland. N₂O is a potent greenhouse gas; understanding its fluxes is essential for addressing global warming. Using a new instrument and flux chambers, we introduce a system to reliably detect low N₂O fluxes and provide recommendations on chamber closure times and flux calculation methods to better quantify N₂O fluxes. We encourage researchers to further investigate N₂O fluxes in low-nutrient environments.