An instrument for quantifying heterogeneous ice nucleation in multiwell plates using infrared emissions to detect freezing

Harrison, Alexander D.; Whale, Thomas F.; Rutledge, Rupert; Lamb, Stephen; Tarn, Mark D.; Porter, Grace C. E.; Adams, Michael P.; McQuaid, James B.; Morris, George J.; Murray, Benjamin J.

doi:https://doi.org/10.5194/amt-11-5629-2018

Articles | Volume 11, issue 10

https://doi.org/10.5194/amt-11-5629-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue:

Fifth International Workshop on Ice Nucleation (FIN) (ACP/AMT...

https://doi.org/10.5194/amt-11-5629-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 11, issue 10

Research article

|

15 Oct 2018

Research article |

| 15 Oct 2018

An instrument for quantifying heterogeneous ice nucleation in multiwell plates using infrared emissions to detect freezing

Alexander D. Harrison, Thomas F. Whale, Rupert Rutledge, Stephen Lamb, Mark D. Tarn, Grace C. E. Porter, Michael P. Adams, James B. McQuaid, George J. Morris, and Benjamin J. Murray

Data sets

Freezing temperature laboratory experiments of individual droplets each contained in a well plate using InfraRed-Nucleation by Immersed Particles Instrument (IR-NIPI) A. D. Harrison, T. F. Whale, R. Rutledge, S. Lamb, M. D. Tarn, G. C. E. Porter, M. Adams, J. B. McQuaid, G. J. Morris, and B. J. Murray https://doi.org/10.5285/858a4b439d7d4466b82ea5215614f135

Short summary

The detection of low concentrations of ice-nucleating particles (INPs) is challenging. Here we present a new technique (IR-NIPI) that is sensitive to low concentrations of INPs (> 0.01 L⁻¹) and uses an infrared camera with a novel calibration to detect the freezing of experimental suspensions. IR-NIPI temperature measurements prove to be robust with a series of comparisons to thermocouple measurements. Experimental comparisons to other freezing assay instruments are also in agreement.