An instrument for quantifying heterogeneous ice nucleation in multiwell plates using infrared emissions to detect freezing

Harrison, Alexander D.; Whale, Thomas F.; Rutledge, Rupert; Lamb, Stephen; Tarn, Mark D.; Porter, Grace C. E.; Adams, Michael P.; McQuaid, James B.; Morris, George J.; Murray, Benjamin J.

doi:https://doi.org/10.5194/amt-11-5629-2018

Articles | Volume 11, issue 10

https://doi.org/10.5194/amt-11-5629-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue:

Fifth International Workshop on Ice Nucleation (FIN) (ACP/AMT...

https://doi.org/10.5194/amt-11-5629-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 11, issue 10

Research article

|

15 Oct 2018

Research article |

| 15 Oct 2018

An instrument for quantifying heterogeneous ice nucleation in multiwell plates using infrared emissions to detect freezing

Alexander D. Harrison, Thomas F. Whale, Rupert Rutledge, Stephen Lamb, Mark D. Tarn, Grace C. E. Porter, Michael P. Adams, James B. McQuaid, George J. Morris, and Benjamin J. Murray

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Final revised paper (published on 15 Oct 2018)
Preprint (discussion started on 13 Jun 2018)

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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- Supplement

RC1: 'Review of Harrison et al.', Anonymous Referee #1, 08 Jul 2018
- AC1: 'Response to RC#1', A. D. Harrison, 06 Aug 2018
RC2: 'Review', Anonymous Referee #2, 10 Jul 2018
- AC2: 'Response to RC #2', A. D. Harrison, 06 Aug 2018
RC3: 'Referee report on the manuscript “An instrument for quantifying heterogeneous ice nucleation in multiwell plates using infrared emissions to detect freezing”', Hinrich Grothe, 12 Jul 2018
- AC3: 'Response to RC #3', A. D. Harrison, 06 Aug 2018
RC4: 'New litereature important for this paper', Hinrich Grothe, 26 Jul 2018
- AC3: 'Response to RC #3', A. D. Harrison, 06 Aug 2018
AC1: 'Response to RC#1', A. D. Harrison, 06 Aug 2018
AC2: 'Response to RC #2', A. D. Harrison, 06 Aug 2018
AC3: 'Response to RC #3', A. D. Harrison, 06 Aug 2018

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

AR by A. D. Harrison on behalf of the Authors (21 Aug 2018)

ED: Referee Nomination & Report Request started (01 Sep 2018) by Mingjin Tang

RR by Anonymous Referee #1 (04 Sep 2018)

RR by Hinrich Grothe (12 Sep 2018)

ED: Publish as is (15 Sep 2018) by Mingjin Tang

AR by A. D. Harrison on behalf of the Authors (25 Sep 2018) Manuscript

Short summary

The detection of low concentrations of ice-nucleating particles (INPs) is challenging. Here we present a new technique (IR-NIPI) that is sensitive to low concentrations of INPs (> 0.01 L⁻¹) and uses an infrared camera with a novel calibration to detect the freezing of experimental suspensions. IR-NIPI temperature measurements prove to be robust with a series of comparisons to thermocouple measurements. Experimental comparisons to other freezing assay instruments are also in agreement.