Articles | Volume 11, issue 10
https://doi.org/10.5194/amt-11-5629-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/amt-11-5629-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
15 Oct 2018
Research article |
| 15 Oct 2018
| 15 Oct 2018
An instrument for quantifying heterogeneous ice nucleation in multiwell plates using infrared emissions to detect freezing
Institute for Climate and Atmospheric Science, School of Earth and
Environment, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK
Thomas F. Whale
Institute for Climate and Atmospheric Science, School of Earth and
Environment, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK
now at: School of Chemistry, University of Leeds, Woodhouse Lane,
Leeds, LS2 9JT, UK
Rupert Rutledge
Asymptote Ltd., GE Healthcare, Sovereign House, Cambridge, CB24 9BZ,
UK
Stephen Lamb
Asymptote Ltd., GE Healthcare, Sovereign House, Cambridge, CB24 9BZ,
UK
Mark D. Tarn
Institute for Climate and Atmospheric Science, School of Earth and
Environment, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK
Grace C. E. Porter
Institute for Climate and Atmospheric Science, School of Earth and
Environment, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK
Michael P. Adams
Institute for Climate and Atmospheric Science, School of Earth and
Environment, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK
James B. McQuaid
Institute for Climate and Atmospheric Science, School of Earth and
Environment, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK
George J. Morris
Asymptote Ltd., GE Healthcare, Sovereign House, Cambridge, CB24 9BZ,
UK
Benjamin J. Murray
CORRESPONDING AUTHOR
Institute for Climate and Atmospheric Science, School of Earth and
Environment, University of Leeds, Woodhouse Lane, Leeds, LS2 9JT, UK
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Cited
19 citations as recorded by crossref.
- Twin-plate Ice Nucleation Assay (TINA) with infrared detection for high-throughput droplet freezing experiments with biological ice nuclei in laboratory and field samples A. Kunert et al. 10.5194/amt-11-6327-2018
- Development of the DRoplet Ice Nuclei Counter Zurich (DRINCZ): validation and application to field-collected snow samples R. David et al. 10.5194/amt-12-6865-2019
- A pyroelectric thermal sensor for automated ice nucleation detection F. Cook et al. 10.5194/amt-13-2785-2020
- Airborne bacterial communities over the Tibetan and Mongolian Plateaus: variations and their possible sources J. Qi et al. 10.1016/j.atmosres.2020.105215
- Measurement report: Introduction to the HyICE-2018 campaign for measurements of ice-nucleating particles and instrument inter-comparison in the Hyytiälä boreal forest Z. Brasseur et al. 10.5194/acp-22-5117-2022
- Resolving the size of ice-nucleating particles with a balloon deployable aerosol sampler: the SHARK G. Porter et al. 10.5194/amt-13-2905-2020
- Pragmatic protocols for working cleanly when measuring ice nucleating particles K. Barry et al. 10.1016/j.atmosres.2020.105419
- Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants K. Murray et al. 10.1038/s41598-022-15545-4
- A Major Combustion Aerosol Event Had a Negligible Impact on the Atmospheric Ice‐Nucleating Particle Population M. Adams et al. 10.1029/2020JD032938
- Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation M. Daily et al. 10.1016/j.cryobiol.2020.02.008
- Development of the drop Freezing Ice Nuclei Counter (FINC), intercomparison of droplet freezing techniques, and use of soluble lignin as an atmospheric ice nucleation standard A. Miller et al. 10.5194/amt-14-3131-2021
- Continuous separation of fungal spores in a microfluidic flow focusing device B. Park et al. 10.1039/C9AN00905A
- Size-dependent ice nucleation by airborne particles during dust events in the eastern Mediterranean N. Reicher et al. 10.5194/acp-19-11143-2019
- Comparative study on immersion freezing utilizing single-droplet levitation methods M. Szakáll et al. 10.5194/acp-21-3289-2021
- Monitoring of freezing patterns within 3D collagen-hydroxyapatite scaffolds using infrared thermography V. Mutsenko et al. 10.1016/j.cryobiol.2023.02.001
- A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format M. Daily et al. 10.1098/rsif.2022.0682
- Revisiting the differential freezing nucleus spectra derived from drop-freezing experiments: methods of calculation, applications, and confidence limits G. Vali 10.5194/amt-12-1219-2019
- Best practices for precipitation sample storage for offline studies of ice nucleation in marine and coastal environments C. Beall et al. 10.5194/amt-13-6473-2020
- On-chip analysis of atmospheric ice-nucleating particles in continuous flow M. Tarn et al. 10.1039/D0LC00251H
19 citations as recorded by crossref.
- Twin-plate Ice Nucleation Assay (TINA) with infrared detection for high-throughput droplet freezing experiments with biological ice nuclei in laboratory and field samples A. Kunert et al. 10.5194/amt-11-6327-2018
- Development of the DRoplet Ice Nuclei Counter Zurich (DRINCZ): validation and application to field-collected snow samples R. David et al. 10.5194/amt-12-6865-2019
- A pyroelectric thermal sensor for automated ice nucleation detection F. Cook et al. 10.5194/amt-13-2785-2020
- Airborne bacterial communities over the Tibetan and Mongolian Plateaus: variations and their possible sources J. Qi et al. 10.1016/j.atmosres.2020.105215
- Measurement report: Introduction to the HyICE-2018 campaign for measurements of ice-nucleating particles and instrument inter-comparison in the Hyytiälä boreal forest Z. Brasseur et al. 10.5194/acp-22-5117-2022
- Resolving the size of ice-nucleating particles with a balloon deployable aerosol sampler: the SHARK G. Porter et al. 10.5194/amt-13-2905-2020
- Pragmatic protocols for working cleanly when measuring ice nucleating particles K. Barry et al. 10.1016/j.atmosres.2020.105419
- Pollen derived macromolecules serve as a new class of ice-nucleating cryoprotectants K. Murray et al. 10.1038/s41598-022-15545-4
- A Major Combustion Aerosol Event Had a Negligible Impact on the Atmospheric Ice‐Nucleating Particle Population M. Adams et al. 10.1029/2020JD032938
- Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation M. Daily et al. 10.1016/j.cryobiol.2020.02.008
- Development of the drop Freezing Ice Nuclei Counter (FINC), intercomparison of droplet freezing techniques, and use of soluble lignin as an atmospheric ice nucleation standard A. Miller et al. 10.5194/amt-14-3131-2021
- Continuous separation of fungal spores in a microfluidic flow focusing device B. Park et al. 10.1039/C9AN00905A
- Size-dependent ice nucleation by airborne particles during dust events in the eastern Mediterranean N. Reicher et al. 10.5194/acp-19-11143-2019
- Comparative study on immersion freezing utilizing single-droplet levitation methods M. Szakáll et al. 10.5194/acp-21-3289-2021
- Monitoring of freezing patterns within 3D collagen-hydroxyapatite scaffolds using infrared thermography V. Mutsenko et al. 10.1016/j.cryobiol.2023.02.001
- A highly active mineral-based ice nucleating agent supports in situ cell cryopreservation in a high throughput format M. Daily et al. 10.1098/rsif.2022.0682
- Revisiting the differential freezing nucleus spectra derived from drop-freezing experiments: methods of calculation, applications, and confidence limits G. Vali 10.5194/amt-12-1219-2019
- Best practices for precipitation sample storage for offline studies of ice nucleation in marine and coastal environments C. Beall et al. 10.5194/amt-13-6473-2020
- On-chip analysis of atmospheric ice-nucleating particles in continuous flow M. Tarn et al. 10.1039/D0LC00251H
Latest update: 24 Apr 2024
Short summary
The detection of low concentrations of ice-nucleating particles (INPs) is challenging. Here we present a new technique (IR-NIPI) that is sensitive to low concentrations of INPs (> 0.01 L−1) and uses an infrared camera with a novel calibration to detect the freezing of experimental suspensions. IR-NIPI temperature measurements prove to be robust with a series of comparisons to thermocouple measurements. Experimental comparisons to other freezing assay instruments are also in agreement.
The detection of low concentrations of ice-nucleating particles (INPs) is challenging. Here we...
