Articles | Volume 12, issue 6
https://doi.org/10.5194/amt-12-3435-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/amt-12-3435-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Automatic pollen recognition with the Rapid-E particle counter: the first-level procedure, experience and next steps
Ingrida Šaulienė
CORRESPONDING AUTHOR
Institute of Regional Development, Šiauliai University, Šiauliai, 76352 Lithuania
Laura Šukienė
Institute of Regional Development, Šiauliai University, Šiauliai, 76352 Lithuania
Gintautas Daunys
Institute of Regional Development, Šiauliai University, Šiauliai, 76352 Lithuania
Gediminas Valiulis
Institute of Regional Development, Šiauliai University, Šiauliai, 76352 Lithuania
Lukas Vaitkevičius
Institute of Regional Development, Šiauliai University, Šiauliai, 76352 Lithuania
Predrag Matavulj
BioSense Institute – Research Institute for Information Technologies
in Biosystems, University of Novi Sad, Novi Sad, 21000, Serbia
Sanja Brdar
BioSense Institute – Research Institute for Information Technologies
in Biosystems, University of Novi Sad, Novi Sad, 21000, Serbia
Marko Panic
BioSense Institute – Research Institute for Information Technologies
in Biosystems, University of Novi Sad, Novi Sad, 21000, Serbia
Branko Sikoparija
BioSense Institute – Research Institute for Information Technologies
in Biosystems, University of Novi Sad, Novi Sad, 21000, Serbia
Bernard Clot
Federal Office of Meteorology and Climatology MeteoSwiss, Payerne,
1530, Switzerland
Benoît Crouzy
Federal Office of Meteorology and Climatology MeteoSwiss, Payerne,
1530, Switzerland
Mikhail Sofiev
Institute of Regional Development, Šiauliai University, Šiauliai, 76352 Lithuania
Finnish Meteorological Institute, Helsinki, 00560, Finland
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- Machine learning methods for low-cost pollen monitoring – Model optimisation and interpretability S. Mills et al. 10.1016/j.scitotenv.2023.165853
- Automatic 3D Pollen Recognition Based on Convolutional Neural Network Z. Wang et al. 10.1155/2021/5577307
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- Single-frame 3D lensless microscopic imaging via deep learning J. Grant-Jacob et al. 10.1364/OE.464678
- Fluorescence Methods for the Detection of Bioaerosols in Their Civil and Military Applications M. Kwaśny et al. 10.3390/s23063339
- RealForAll: real-time system for automatic detection of airborne pollen D. Tešendić et al. 10.1080/17517575.2020.1793391
- On the application of scattering matrix measurements to detection and identification of major types of airborne aerosol particles: Volcanic ash, desert dust and pollen J. Gómez Martín et al. 10.1016/j.jqsrt.2021.107761
- A first evaluation of multiple automatic pollen monitors run in parallel F. Tummon et al. 10.1007/s10453-021-09729-0
- Pollen and sub-pollen particles: External interactions shaping the allergic potential of pollen S. Venkatesan et al. 10.1016/j.scitotenv.2024.171593
- The EUMETNET AutoPollen programme: establishing a prototype automatic pollen monitoring network in Europe B. Clot et al. 10.1007/s10453-020-09666-4
- Measurement report: Atmospheric fluorescent bioaerosol concentrations measured during 18 months in a coniferous forest in the south of Sweden M. Petersson Sjögren et al. 10.5194/acp-23-4977-2023
- Clustering approach for the analysis of the fluorescent bioaerosol collected by an automatic detector G. Daunys et al. 10.1371/journal.pone.0247284
- Why should we care about high temporal resolution monitoring of bioaerosols in ambient air? M. Smith et al. 10.1016/j.scitotenv.2022.154231
- Automatic pollen monitoring: first insights from hourly data C. Chappuis et al. 10.1007/s10453-019-09619-6
- A demonstration project of Global Alliance against Chronic Respiratory Diseases: Prediction of interactions between air pollution and allergen exposure—the Mobile Airways Sentinel NetworK-Impact of air POLLution on Asthma and Rhinitis approach M. Sofiev et al. 10.1097/CM9.0000000000000916
- Towards standardisation of automatic pollen and fungal spore monitoring: best practises and guidelines F. Tummon et al. 10.1007/s10453-022-09755-6
- Automatic particle detectors lead to a new generation in plant diversity investigation I. ŠAULIENĖ et al. 10.15835/nbha49312444
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- A global survey addressing sustainability of pollen monitoring D. Dwarakanath et al. 10.1016/j.waojou.2024.100997
- Detection of Airborne Biological Particles in Indoor Air Using a Real-Time Advanced Morphological Parameter UV-LIF Spectrometer and Gradient Boosting Ensemble Decision Tree Classifiers I. Crawford et al. 10.3390/atmos11101039
- Towards European automatic bioaerosol monitoring: Comparison of 9 automatic pollen observational instruments with classic Hirst-type traps J. Maya-Manzano et al. 10.1016/j.scitotenv.2022.161220
- Electro-Optical Classification of Pollen Grains via Microfluidics and Machine Learning M. DaOrazio et al. 10.1109/TBME.2021.3109384
- Constructing a pollen proxy from low-cost Optical Particle Counter (OPC) data processed with Neural Networks and Random Forests S. Mills et al. 10.1016/j.scitotenv.2023.161969
- False positives: handling them operationally for automatic pollen monitoring B. Crouzy et al. 10.1007/s10453-022-09757-4
- The role of automatic pollen and fungal spore monitoring across major end-user domains F. Tummon et al. 10.1007/s10453-024-09820-2
- Spatial Variation of Airborne Pollen Concentrations Locally around Brussels City, Belgium, during a Field Campaign in 2022–2023, Using the Automatic Sensor Beenose J. Renard et al. 10.3390/s24123731
- On the measurement uncertainty of Hirst-type volumetric pollen and spore samplers S. Adamov et al. 10.1007/s10453-021-09724-5
- Pollen detection through integrated microfluidics and smartphone-driven deep learning systems K. Chen et al. 10.1016/j.rineng.2024.102867
- Real-time pollen monitoring using digital holography E. Sauvageat et al. 10.5194/amt-13-1539-2020
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- Desert dust has a notable impact on aerobiological measurements in Europe B. Šikoparija 10.1016/j.aeolia.2020.100636
- Airborne Prokaryotic, Fungal and Eukaryotic Communities of an Urban Environment in the UK H. Song et al. 10.3390/atmos13081212
- Pollen observations at four EARLINET stations during the ACTRIS-COVID-19 campaign X. Shang et al. 10.5194/acp-22-3931-2022
- The need for Pan‐European automatic pollen and fungal spore monitoring: A stakeholder workshop position paper F. Tummon et al. 10.1002/clt2.12015
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- Towards an Automatic Pollen Detection System in Ambient Air Using Scattering Functions in the Visible Domain J. Renard et al. 10.3390/s22134984
- Variability between Hirst-type pollen traps is reduced by resistance-free flow adjustment M. Triviño et al. 10.1007/s10453-023-09790-x
- Automatic detection of airborne pollen: an overview J. Buters et al. 10.1007/s10453-022-09750-x
- Multi-Input Convolutional Neural Networks for Automatic Pollen Classification M. Boldeanu et al. 10.3390/app112411707
- Designing an automatic pollen monitoring network for direct usage of observations to reconstruct the concentration fields M. Sofiev et al. 10.1016/j.scitotenv.2023.165800
- Real-time sensing of bioaerosols: Review and current perspectives J. Huffman et al. 10.1080/02786826.2019.1664724
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- How to select the optimal monitoring locations for an aerobiological network: A case of study in central northwest of Spain A. Rodríguez-Fernández et al. 10.1016/j.scitotenv.2022.154370
- Alternaria spore exposure in Bavaria, Germany, measured using artificial intelligence algorithms in a network of BAA500 automatic pollen monitors M. González-Alonso et al. 10.1016/j.scitotenv.2022.160180
- Real-time automatic detection of starch particles in ambient air B. Šikoparija et al. 10.1016/j.agrformet.2022.109034
- Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy S. Brdar et al. 10.1038/s41598-023-30064-6
- Estimation of pollen counts from light scattering intensity when sampling multiple pollen taxa – establishment of an automated multi-taxa pollen counting estimation system (AME system) K. Miki & S. Kawashima 10.5194/amt-14-685-2021
- Towards automatic airborne pollen monitoring: From commercial devices to operational by mitigating class-imbalance in a deep learning approach J. Schaefer et al. 10.1016/j.scitotenv.2021.148932
- Application of High-Throughput Screening Raman Spectroscopy (HTS-RS) for Label-Free Identification and Molecular Characterization of Pollen A. Mondol et al. 10.3390/s19204428
- Total Bioaerosol Detection by a Succinimidyl-Ester-Functionalized Plasmonic Biosensor To Reveal Different Characteristics at Three Locations in Switzerland G. Qiu et al. 10.1021/acs.est.9b05184
- A Laboratory Evaluation of the New Automated Pollen Sensor Beenose: Pollen Discrimination Using Machine Learning Techniques H. El Azari et al. 10.3390/s23062964
- Air Sampling and Analysis of Aeroallergens: Current and Future Approaches E. Levetin et al. 10.1007/s11882-023-01073-2
- Monitoring techniques for pollen allergy risk assessment C. Suanno et al. 10.1016/j.envres.2021.111109
- Development and application of a method to classify airborne pollen taxa concentration using light scattering data K. Miki et al. 10.1038/s41598-021-01919-7
- Bioaerosols in the atmosphere at two sites in Northern Europe in spring 2021: Outline of an experimental campaign M. Sofiev et al. 10.1016/j.envres.2022.113798
- Aerosol physical characterization: A review on the current state of aerosol documentary standards and calibration strategies K. Vasilatou et al. 10.1016/j.jaerosci.2024.106483
- Laboratory evaluation of the scattering matrix of ragweed, ash, birch and pine pollen towards pollen classification D. Cholleton et al. 10.5194/amt-15-1021-2022
- Global Climate Change and Pollen Aeroallergens J. Davies et al. 10.1016/j.iac.2020.09.002
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- In-flight sensing of pollen grains via laser scattering and deep learning J. Grant-Jacob et al. 10.1088/2631-8695/abfdf8
- Comparison of computer vision models in application to pollen classification using light scattering G. Daunys et al. 10.1007/s10453-022-09769-0
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- A portable flow tube homogenizer for aerosol mixing in the sub-micrometre and lower micrometre particle size range S. Horender et al. 10.1088/1361-6501/ac81a1
- Imaging Flow Cytometry as a Quick and Effective Identification Technique of Pollen Grains from Betulaceae, Oleaceae, Urticaceae and Asteraceae I. Gierlicka et al. 10.3390/cells11040598
- Pollen clustering strategies using a newly developed single-particle fluorescence spectrometer B. Swanson & J. Huffman 10.1080/02786826.2019.1711357
- On possibilities of assimilation of near-real-time pollen data by atmospheric composition models M. Sofiev 10.1007/s10453-019-09583-1
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- Extension of WRF-Chem for birch pollen modelling—a case study for Poland M. Werner et al. 10.1007/s00484-020-02045-1
72 citations as recorded by crossref.
- Integration of reference data from different Rapid-E devices supports automatic pollen detection in more locations P. Matavulj et al. 10.1016/j.scitotenv.2022.158234
- Machine learning methods for low-cost pollen monitoring – Model optimisation and interpretability S. Mills et al. 10.1016/j.scitotenv.2023.165853
- Automatic 3D Pollen Recognition Based on Convolutional Neural Network Z. Wang et al. 10.1155/2021/5577307
- Comprehensive insights into advances in ambient bioaerosols sampling, analysis and factors influencing bioaerosols composition B. Sajjad et al. 10.1016/j.envpol.2023.122473
- Single-frame 3D lensless microscopic imaging via deep learning J. Grant-Jacob et al. 10.1364/OE.464678
- Fluorescence Methods for the Detection of Bioaerosols in Their Civil and Military Applications M. Kwaśny et al. 10.3390/s23063339
- RealForAll: real-time system for automatic detection of airborne pollen D. Tešendić et al. 10.1080/17517575.2020.1793391
- On the application of scattering matrix measurements to detection and identification of major types of airborne aerosol particles: Volcanic ash, desert dust and pollen J. Gómez Martín et al. 10.1016/j.jqsrt.2021.107761
- A first evaluation of multiple automatic pollen monitors run in parallel F. Tummon et al. 10.1007/s10453-021-09729-0
- Pollen and sub-pollen particles: External interactions shaping the allergic potential of pollen S. Venkatesan et al. 10.1016/j.scitotenv.2024.171593
- The EUMETNET AutoPollen programme: establishing a prototype automatic pollen monitoring network in Europe B. Clot et al. 10.1007/s10453-020-09666-4
- Measurement report: Atmospheric fluorescent bioaerosol concentrations measured during 18 months in a coniferous forest in the south of Sweden M. Petersson Sjögren et al. 10.5194/acp-23-4977-2023
- Clustering approach for the analysis of the fluorescent bioaerosol collected by an automatic detector G. Daunys et al. 10.1371/journal.pone.0247284
- Why should we care about high temporal resolution monitoring of bioaerosols in ambient air? M. Smith et al. 10.1016/j.scitotenv.2022.154231
- Automatic pollen monitoring: first insights from hourly data C. Chappuis et al. 10.1007/s10453-019-09619-6
- A demonstration project of Global Alliance against Chronic Respiratory Diseases: Prediction of interactions between air pollution and allergen exposure—the Mobile Airways Sentinel NetworK-Impact of air POLLution on Asthma and Rhinitis approach M. Sofiev et al. 10.1097/CM9.0000000000000916
- Towards standardisation of automatic pollen and fungal spore monitoring: best practises and guidelines F. Tummon et al. 10.1007/s10453-022-09755-6
- Automatic particle detectors lead to a new generation in plant diversity investigation I. ŠAULIENĖ et al. 10.15835/nbha49312444
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- Towards a UK Airborne Bioaerosol Climatology: Real-Time Monitoring Strategies for High Time Resolution Bioaerosol Classification and Quantification I. Crawford et al. 10.3390/atmos14081214
- Pollen classification using a single particle fluorescence spectroscopy technique B. Swanson et al. 10.1080/02786826.2022.2142510
- Assessment of real-time bioaerosol particle counters using reference chamber experiments G. Lieberherr et al. 10.5194/amt-14-7693-2021
- A global survey addressing sustainability of pollen monitoring D. Dwarakanath et al. 10.1016/j.waojou.2024.100997
- Detection of Airborne Biological Particles in Indoor Air Using a Real-Time Advanced Morphological Parameter UV-LIF Spectrometer and Gradient Boosting Ensemble Decision Tree Classifiers I. Crawford et al. 10.3390/atmos11101039
- Towards European automatic bioaerosol monitoring: Comparison of 9 automatic pollen observational instruments with classic Hirst-type traps J. Maya-Manzano et al. 10.1016/j.scitotenv.2022.161220
- Electro-Optical Classification of Pollen Grains via Microfluidics and Machine Learning M. DaOrazio et al. 10.1109/TBME.2021.3109384
- Constructing a pollen proxy from low-cost Optical Particle Counter (OPC) data processed with Neural Networks and Random Forests S. Mills et al. 10.1016/j.scitotenv.2023.161969
- False positives: handling them operationally for automatic pollen monitoring B. Crouzy et al. 10.1007/s10453-022-09757-4
- The role of automatic pollen and fungal spore monitoring across major end-user domains F. Tummon et al. 10.1007/s10453-024-09820-2
- Spatial Variation of Airborne Pollen Concentrations Locally around Brussels City, Belgium, during a Field Campaign in 2022–2023, Using the Automatic Sensor Beenose J. Renard et al. 10.3390/s24123731
- On the measurement uncertainty of Hirst-type volumetric pollen and spore samplers S. Adamov et al. 10.1007/s10453-021-09724-5
- Pollen detection through integrated microfluidics and smartphone-driven deep learning systems K. Chen et al. 10.1016/j.rineng.2024.102867
- Real-time pollen monitoring using digital holography E. Sauvageat et al. 10.5194/amt-13-1539-2020
- Optimisation of bioaerosol sampling using an ultralight aircraft: A novel approach in determining the 3-D atmospheric biodiversity M. Plaza et al. 10.1016/j.heliyon.2024.e38924
- Desert dust has a notable impact on aerobiological measurements in Europe B. Šikoparija 10.1016/j.aeolia.2020.100636
- Airborne Prokaryotic, Fungal and Eukaryotic Communities of an Urban Environment in the UK H. Song et al. 10.3390/atmos13081212
- Pollen observations at four EARLINET stations during the ACTRIS-COVID-19 campaign X. Shang et al. 10.5194/acp-22-3931-2022
- The need for Pan‐European automatic pollen and fungal spore monitoring: A stakeholder workshop position paper F. Tummon et al. 10.1002/clt2.12015
- High-Resolution Fluorescence Spectra of Airborne Biogenic Secondary Organic Aerosols: Comparisons to Primary Biological Aerosol Particles and Implications for Single-Particle Measurements M. Zhang et al. 10.1021/acs.est.1c02536
- Towards an Automatic Pollen Detection System in Ambient Air Using Scattering Functions in the Visible Domain J. Renard et al. 10.3390/s22134984
- Variability between Hirst-type pollen traps is reduced by resistance-free flow adjustment M. Triviño et al. 10.1007/s10453-023-09790-x
- Automatic detection of airborne pollen: an overview J. Buters et al. 10.1007/s10453-022-09750-x
- Multi-Input Convolutional Neural Networks for Automatic Pollen Classification M. Boldeanu et al. 10.3390/app112411707
- Designing an automatic pollen monitoring network for direct usage of observations to reconstruct the concentration fields M. Sofiev et al. 10.1016/j.scitotenv.2023.165800
- Real-time sensing of bioaerosols: Review and current perspectives J. Huffman et al. 10.1080/02786826.2019.1664724
- Aeroallergen Monitoring by the National Allergy Bureau: A Review of the Past and a Look Into the Future E. Levetin et al. 10.1016/j.jaip.2022.11.026
- Classification accuracy and compatibility across devices of a new Rapid-E+ flow cytometer B. Sikoparija et al. 10.5194/amt-17-5051-2024
- Automatisches Pollenmonitoring in Deutschland J. Buters et al. 10.1007/s15007-020-2527-0
- Manual and automatic quantification of airborne fungal spores during wheat harvest period I. Simović et al. 10.1007/s10453-023-09788-5
- Bioaerosol field measurements: Challenges and perspectives in outdoor studies T. Šantl-Temkiv et al. 10.1080/02786826.2019.1676395
- How to select the optimal monitoring locations for an aerobiological network: A case of study in central northwest of Spain A. Rodríguez-Fernández et al. 10.1016/j.scitotenv.2022.154370
- Alternaria spore exposure in Bavaria, Germany, measured using artificial intelligence algorithms in a network of BAA500 automatic pollen monitors M. González-Alonso et al. 10.1016/j.scitotenv.2022.160180
- Real-time automatic detection of starch particles in ambient air B. Šikoparija et al. 10.1016/j.agrformet.2022.109034
- Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy S. Brdar et al. 10.1038/s41598-023-30064-6
- Estimation of pollen counts from light scattering intensity when sampling multiple pollen taxa – establishment of an automated multi-taxa pollen counting estimation system (AME system) K. Miki & S. Kawashima 10.5194/amt-14-685-2021
- Towards automatic airborne pollen monitoring: From commercial devices to operational by mitigating class-imbalance in a deep learning approach J. Schaefer et al. 10.1016/j.scitotenv.2021.148932
- Application of High-Throughput Screening Raman Spectroscopy (HTS-RS) for Label-Free Identification and Molecular Characterization of Pollen A. Mondol et al. 10.3390/s19204428
- Total Bioaerosol Detection by a Succinimidyl-Ester-Functionalized Plasmonic Biosensor To Reveal Different Characteristics at Three Locations in Switzerland G. Qiu et al. 10.1021/acs.est.9b05184
- A Laboratory Evaluation of the New Automated Pollen Sensor Beenose: Pollen Discrimination Using Machine Learning Techniques H. El Azari et al. 10.3390/s23062964
- Air Sampling and Analysis of Aeroallergens: Current and Future Approaches E. Levetin et al. 10.1007/s11882-023-01073-2
- Monitoring techniques for pollen allergy risk assessment C. Suanno et al. 10.1016/j.envres.2021.111109
- Development and application of a method to classify airborne pollen taxa concentration using light scattering data K. Miki et al. 10.1038/s41598-021-01919-7
- Bioaerosols in the atmosphere at two sites in Northern Europe in spring 2021: Outline of an experimental campaign M. Sofiev et al. 10.1016/j.envres.2022.113798
- Aerosol physical characterization: A review on the current state of aerosol documentary standards and calibration strategies K. Vasilatou et al. 10.1016/j.jaerosci.2024.106483
- Laboratory evaluation of the scattering matrix of ragweed, ash, birch and pine pollen towards pollen classification D. Cholleton et al. 10.5194/amt-15-1021-2022
- Global Climate Change and Pollen Aeroallergens J. Davies et al. 10.1016/j.iac.2020.09.002
- Advanced CNN Architectures for Pollen Classification: Design and Comprehensive Evaluation P. Matavulj et al. 10.1080/08839514.2022.2157593
- In-flight sensing of pollen grains via laser scattering and deep learning J. Grant-Jacob et al. 10.1088/2631-8695/abfdf8
- Comparison of computer vision models in application to pollen classification using light scattering G. Daunys et al. 10.1007/s10453-022-09769-0
- Isolating the species element in grass pollen allergy: A review C. Frisk et al. 10.1016/j.scitotenv.2023.163661
- A portable flow tube homogenizer for aerosol mixing in the sub-micrometre and lower micrometre particle size range S. Horender et al. 10.1088/1361-6501/ac81a1
- Imaging Flow Cytometry as a Quick and Effective Identification Technique of Pollen Grains from Betulaceae, Oleaceae, Urticaceae and Asteraceae I. Gierlicka et al. 10.3390/cells11040598
5 citations as recorded by crossref.
- Pollen clustering strategies using a newly developed single-particle fluorescence spectrometer B. Swanson & J. Huffman 10.1080/02786826.2019.1711357
- On possibilities of assimilation of near-real-time pollen data by atmospheric composition models M. Sofiev 10.1007/s10453-019-09583-1
- Pollen classification using a single particle fluorescence spectroscopy technique B. Swanson et al. 10.1080/02786826.2022.2142510
- Multi-point analysis of airborne Japanese cedar (Cryptomeria japonica D. Don) pollen by Pollen Robo and the relationship between pollen count and the severity of symptoms Y. Takahashi et al. 10.1007/s10453-019-09603-0
- Extension of WRF-Chem for birch pollen modelling—a case study for Poland M. Werner et al. 10.1007/s00484-020-02045-1
Latest update: 14 Dec 2024
Short summary
The goal is to evaluate the capabilities of the new Rapid-E monitor and to construct a first-level pollen recognition algorithm. The output data were treated with ANN aiming at classification of the injected pollen. Algorithms based on scattering and fluorescence data alone fall short of acceptable quality. The combinations of these exceeded 80 % accuracy for 5 out of 11 pollen species. Constructing multistep algorithms with sequential discrimination of pollen can be a possible way forward.
The goal is to evaluate the capabilities of the new Rapid-E monitor and to construct a...