Articles | Volume 11, issue 7
Research article
06 Jul 2018
Research article |  | 06 Jul 2018

Characterization of steady-state fluorescence properties of polystyrene latex spheres using off- and online spectroscopic methods

Tobias Könemann, Nicole J. Savage, J. Alex Huffman, and Christopher Pöhlker

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Cited articles

Agranovski, V., Ristovski, Z., Hargreaves, M., Blackall, P., and Morawska, L.: Real-time measurement of bacterial aerosols with the UVAPS: performance evaluation, J. Aerosol Sci., 34, 301–317,, 2003. 
Andreae, M. O. and Crutzen, P. J.: Atmospheric Aerosols: Biogeochemical Sources and Role in Atmospheric Chemistry, Science, 276, 1052–1058,, 1997. 
Bhangar, S., Huffman, J. A., and Nazaroff, W. W.: Size-resolved fluorescent biological aerosol particle concentrations and occupant emissions in a university classroom, Indoor Air, 24, 604–617, 2014. 
Brosseau, L. M., Vesley, D., Rice, N., Goodell, K., Nellis, M., and Hairston, P.: Differences in detected fluorescence among several bacterial species measured with a direct-reading particle sizer and fluorescence detector, Aerosol Sci. Tech., 32, 545–558, 2000. 
Charreyre, M.-T., Yekta, A., Winnik, M. A., Delair, T., and Pichot, C.: Fluorescence energy transfer from fluorescein to tetramethylrhodamine covalently bound to the surface of polystyrene latex particles, Langmuir, 11, 2423–2428, 1995. 
Short summary
This study presents an overview of fluorescence properties of polystyrene latex spheres (PSLs), which are widely used in numerous scientific disciplines. By using different spectroscopic techniques, we show that the fluorescence landscape of PSLs is more complex than the information provided by manufacturers may imply. By understanding general fluorescence properties of PSLs, individual researchers may probe specific spectral features important to the operation of their own instruments.