Articles | Volume 14, issue 6
https://doi.org/10.5194/amt-14-4461-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/amt-14-4461-2021
© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Comparative characterization of the performance of bio-aerosol nebulizers in connection with atmospheric simulation chambers
Silvia G. Danelli
Dipartimento di Fisica – Università di Genova, via Dodecaneso 33, 16146, Genova, Italy
INFN – Sezione di Genova, via Dodecaneso 33, 16146, Genova, Italy
Marco Brunoldi
Dipartimento di Fisica – Università di Genova, via Dodecaneso 33, 16146, Genova, Italy
INFN – Sezione di Genova, via Dodecaneso 33, 16146, Genova, Italy
Dipartimento di Fisica – Università di Genova, via Dodecaneso 33, 16146, Genova, Italy
INFN – Sezione di Genova, via Dodecaneso 33, 16146, Genova, Italy
Franco Parodi
INFN – Sezione di Genova, via Dodecaneso 33, 16146, Genova, Italy
Virginia Vernocchi
Dipartimento di Fisica – Università di Genova, via Dodecaneso 33, 16146, Genova, Italy
INFN – Sezione di Genova, via Dodecaneso 33, 16146, Genova, Italy
Paolo Prati
Dipartimento di Fisica – Università di Genova, via Dodecaneso 33, 16146, Genova, Italy
INFN – Sezione di Genova, via Dodecaneso 33, 16146, Genova, Italy
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Cited articles
Alsved, M., Bourouiba, L., Duchaine, C., Löndahl, J., Marr, L.C.,
Parker, S. T., Prussin II, A. J., and Thomas, R. J.: Natural sources and
experimental generation of bioaerosols: Challenges and Perspectives, Aerosol
Sci. Tech., 54, 547–571, https://doi.org/10.1080/02786826.2019.1682509, 2019.
Amato, P., Joly, M., Schaupp, C., Attard, E., Möhler, O., Morris, C. E., Brunet, Y., and Delort, A.-M.: Survival and ice nucleation activity of bacteria as aerosols in a cloud simulation chamber, Atmos. Chem. Phys., 15, 6455–6465, https://doi.org/10.5194/acp-15-6455-2015, 2015.
Becker, K. H.: Overview on the Development of Chambers for the Study of
Atmospheric Chemical Processes, in: Environmental Simulation Chambers:
Application to Atmospheric Chemical Processes, edited by: Barnes, I. and Rudzinski, K. J., Springer, Amsterdam, the Netherlands, 1–26, 2016.
Bowers, R. M., McLetchie, S., Knight, R., and Fierer, N.: Spatial
variability in airborne bacterial communities across land-use types and
their relationship to the bacterial communities of potential source
environments, Isme J., 5, 601–612, 2010.
Brotto, P., Repetto, B., Formenti, P., Pangui, E., Livet, A., Bousserrhine,
N., Martini, I., Varnier, O., Doussin, J.-F., and Prati, P.: Use of an
atmospheric simulation chamber for bioaerosol investigation: a feasibility
study, Aerobiologia, 31, 445–455, 2015.
Burrows, S. M., Elbert, W., Lawrence, M. G., and Pöschl, U.: Bacteria in the global atmosphere – Part 1: Review and synthesis of literature data for different ecosystems, Atmos. Chem. Phys., 9, 9263–9280, https://doi.org/10.5194/acp-9-9263-2009, 2009.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/EYEN-W128, 2021a.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/E8HV-3950, 2021b.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/YW3E-A394, 2021c.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/T1PF-T863, 2021d.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/CG6W-YE23, 2021e.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/5RE2-JG98, 2021f.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/FWZZ-2F76, 2021g.
Danelli, S., Massabò, D., and Prati, P.: Atmospheric simulation chamber study: escherichia coli – Reference experiment, AERIS [data set], https://doi.org/10.25326/RGE1-X868, 2021h.
Després, V. R., Huffman, J. A., Burrows, S. M., Hoose, C., Safatov, A.
S., Buryak, G., Fröhlich-Nowoisky, J., Elbert, W., Andreae, M. O.,
Pöschl, U., and Jaenicke, R.: Primary biological aerosol particles in
the atmosphere: a review, Tellus B, 64, 15598, https://doi.org/10.3402/tellusb.v64i0.15598, 2012.
Federici, E., Petroselli, C., Montalbani, E., Casagrande, C., Ceci, E.,
Moroni, B., Porta, G.L., Castellini, S., Selvaggi, R., Sebastiani, B.,
Crocchianti, S., Gandolfi, I., Franzetti, A., and Cappelletti, D.: Airborne
bacteria and persistent organic pollutants associated with an intense
Saharan dust event in the Central Mediterranean, Sci. Total
Environ., 645, 401–410, 2018.
Finlayson-Pitts, B. J. and Pitts Jr., J. N.: Chemistry of the upper and
lower atmosphere: Theory, experiments and applications, Academic Press, San
Diego, CA, USA, 2000.
Jang, J., Hur, H., Sadowsky, M., Byappanahalli, M., Yan, T., and Ishii, S.:
Environmental Escherichia coli: ecology and public health implications – a review, J. Appl. Microbiol., 123, 570–581, 2017.
Joung, Y., Ge, Z., and Buie, C.: Bioaerosol generation by raindrops on soil,
Nat. Commun., 8, 14668, https://doi.org/10.1038/ncomms14668, 2017.
Lee, B. U. and Kim, S. S.: Sampling E. coli and B. subtilis bacteria bioaerosols by a new type of impactor with a cooled impaction plate, J. Aerosol Sci., 34, 1097–1100, 2003.
Lee, B. U., Kim, S. H., and Kim, S. S.: Hygroscopic growth of E. coli and B. subtilis bioaerosols, J. Aerosol Sci., 33, 1721–1723, 2002.
Mainelis, G., Berry, D., An, H. R., Yao, M. S., De Voe, K., Fennell, D. E., and Jaeger, R.: Design and performance of a single pass bubbling bioaerosol
generator, Atmos. Environ., 39, 3521–3533, 2005.
Marthi, B., Fieland, V. P., Walter, M., and Seidler, R. J.: Survival of bacteria
during aerosolization, Appl. Environ. Microbiol., 56, 3463–3467, 1990.
Massabò, D., Danelli, S. G., Brotto, P., Comite, A., Costa, C., Di Cesare, A., Doussin, J. F., Ferraro, F., Formenti, P., Gatta, E., Negretti, L., Oliva, M., Parodi, F., Vezzulli, L., and Prati, P.: ChAMBRe: a new atmospheric simulation chamber for aerosol modelling and bio-aerosol research, Atmos. Meas. Tech., 11, 5885–5900, https://doi.org/10.5194/amt-11-5885-2018, 2018.
Reponen, T., Willeke, K., Ulevicius, V., Grinshpun, S. A., and Donnelly, J.:
Techniques for Dispersion of Microorganisms into Air, Aerosol Sci. Tech.,
27, 405–421, 1997.
Simon, X., Duquenne, P., Koehler, V., Piernot, C., Coulais, C., and Faure,
M.: Aerosolisation of Escherichia coli and associated endotoxin using an improved bubbling
bioaerosol generator, J. Aerosol Sci., 42, 517–531, 2011.
Terzieva, S., Donnelly, J., Ulevicius, V., Grinshpun, S. A., Willeke, K.,
Stelma, G. N., and Brenner, K. P.: Comparison of methods for detection and
enumeration of airborne microorganisms collected by liquid impingement,
Appl. Environ. Microbiol., 62, 2264–2272, 1996.
Thomas, R. J., Webber, D., Hopkins, R., Frost, A., Laws, T., Jayasekera, P. N., and
Atkins, T.: The cell membrane as a major site of damage during aerosolization
of Escherichia coli, Appl. Environ. Microbiol., 77, 920–925, 2011.
Zhen, H., Han, T., Fennell, D. E., and Mainelis, G.: A systematic comparison of four bioaerosol generators: Affect on culturability and cell membrane
integrity when aerosolizing Escherichia coli bacteria, J. Aerosol Sci., 70,
67–79, 2014.
Zheng, Y. and Yao, M.: Liquid impinger BioSampler's performance for
size-resolved viable bioaerosol particles, J. Aerosol Sci., 106, 34–42,
2017.
Short summary
Experiments conducted inside confined artificial environments, such as atmospheric simulation chambers (ASCs), where atmospheric conditions and composition are controlled, can provide valuable information on bio-aerosol viability, dispersion, and impact. We focus here on the reproducible aerosolization and injection of viable microorganisms into an ASC, the first and crucial step of any experimental protocol to expose bio-aerosols to different atmospheric conditions.
Experiments conducted inside confined artificial environments, such as atmospheric simulation...