Articles | Volume 10, issue 11
https://doi.org/10.5194/amt-10-4279-2017
https://doi.org/10.5194/amt-10-4279-2017
Research article
 | 
10 Nov 2017
Research article |  | 10 Nov 2017

Systematic characterization and fluorescence threshold strategies for the wideband integrated bioaerosol sensor (WIBS) using size-resolved biological and interfering particles

Nicole J. Savage, Christine E. Krentz, Tobias Könemann, Taewon T. Han, Gediminas Mainelis, Christopher Pöhlker, and J. Alex Huffman

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Cited articles

Abdel-Shafy, H. I. and Mansour, M. S. M.: A review on polycyclic aromatic hydrocarbons: Source, environmental impact, effect on human health and remediation, Egyptian Journal of Petroleum, 25, 107–123, https://doi.org/10.1016/j.ejpe.2015.03.011, 2016.
Agranovski, V., Ristovski, Z., Hargreaves, M., Blackall, P. J., and Morawska, L.: Real-time measurement of bacterial aerosols with the UVAPS: performance evaluation, J. Aerosol Sci., 34, 301–317, https://doi.org/10.1016/s0021-8502(02)00181-7, 2003.
Agranovski, V., Ristovski, Z. D., Ayoko, G. A., and Morawska, L.: Performance evaluation of the UVAPS in measuring biological aerosols: Fluorescence spectra from NAD(P)H coenzymes and riboflavin, Aerosol Sci. Tech., 38, 354–364, https://doi.org/10.1080/02786820490437505, 2004.
Agranovski, V., and Ristovski, Z. D.: Real-time monitoring of viable bioaerosols: capability of the UVAPS to predict the amount of individual microorganisms in aerosol particles, J. Aerosol Sci., 36, 665–676, https://doi.org/10.1016/j.jaerosci.2004.12.005, 2005.
Aizawa, T. and Kosaka, H.: Investigation of early soot formation process in a diesel spray flame via excitation-emission matrix using a multi-wavelength laser source, Int. J. Engine Res., 9, 79–96, https://doi.org/10.1243/14680874jer01407, 2008.
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Short summary
We present a comprehensive characterization of a commonly used commercial instrument (WIBS) for the real-time detection of fluorescent bioaerosols and suggest improved analysis and threshold strategies. Summaries of both biological and potential interfering, non-biological particles (70 aerosol types in total) are discussed in detail. The strategies we suggest will minimize interference from non-biological particles and will aid instrument users’ interpretation of ambient particle data.