Articles | Volume 14, issue 12
https://doi.org/10.5194/amt-14-7579-2021
https://doi.org/10.5194/amt-14-7579-2021
Research article
 | 
07 Dec 2021
Research article |  | 07 Dec 2021

A semi-automated instrument for cellular oxidative potential evaluation (SCOPE) of water-soluble extracts of ambient particulate matter

Sudheer Salana, Yixiang Wang, Joseph V. Puthussery, and Vishal Verma

Related authors

An interlaboratory comparison to quantify oxidative potential measurement in aerosol particles: challenges and recommendations for harmonisation
Pamela A. Dominutti, Jean-Luc Jaffrezo, Anouk Marsal, Takoua Mhadhbi, Rhabira Elazzouzi, Camille Rak, Fabrizia Cavalli, Jean-Philippe Putaud, Aikaterini Bougiatioti, Nikolaos Mihalopoulos, Despina Paraskevopoulou, Ian S. Mudway, Athanasios Nenes, Kaspar R. Daellenbach, Catherine Banach, Steven J. Campbell, Hana Cigánková, Daniele Contini, Greg Evans, Maria Georgopoulou, Manuella Ghanem, Drew A. Glencross, Maria Rachele Guascito, Hartmut Herrmann, Saima Iram, Maja Jovanović, Milena Jovašević-Stojanović, Markus Kalberer, Ingeborg M. Kooter, Suzanne E. Paulson, Anil Patel, Esperanza Perdrix, Maria Chiara Pietrogrande, Pavel Mikuška, Jean-Jacques Sauvain, Aikaterina Seitanidi, Pourya Shahpoury, Eduardo J. S. Souza, Sarah Steimer, Svetlana Stevanovic, Guillaume Suarez, P. S. Ganesh Subramanian, Battist Utinger, Marloes F. van Os, Vishal Verma, Xing Wang, Rodney J. Weber, Yuhan Yang, Xavier Querol, Gerard Hoek, Roy M. Harrison, and Gaëlle Uzu
Atmos. Meas. Tech. Discuss., https://doi.org/10.5194/amt-2024-107,https://doi.org/10.5194/amt-2024-107, 2024
Revised manuscript accepted for AMT
Short summary
Highly time-resolved chemical speciation and source apportionment of organic aerosol components in Delhi, India, using extractive electrospray ionization mass spectrometry
Varun Kumar, Stamatios Giannoukos, Sophie L. Haslett, Yandong Tong, Atinderpal Singh, Amelie Bertrand, Chuan Ping Lee, Dongyu S. Wang, Deepika Bhattu, Giulia Stefenelli, Jay S. Dave, Joseph V. Puthussery, Lu Qi, Pawan Vats, Pragati Rai, Roberto Casotto, Rangu Satish, Suneeti Mishra, Veronika Pospisilova, Claudia Mohr, David M. Bell, Dilip Ganguly, Vishal Verma, Neeraj Rastogi, Urs Baltensperger, Sachchida N. Tripathi, André S. H. Prévôt, and Jay G. Slowik
Atmos. Chem. Phys., 22, 7739–7761, https://doi.org/10.5194/acp-22-7739-2022,https://doi.org/10.5194/acp-22-7739-2022, 2022
Short summary
Spatiotemporal variability in the oxidative potential of ambient fine particulate matter in the Midwestern United States
Haoran Yu, Joseph Varghese Puthussery, Yixiang Wang, and Vishal Verma
Atmos. Chem. Phys., 21, 16363–16386, https://doi.org/10.5194/acp-21-16363-2021,https://doi.org/10.5194/acp-21-16363-2021, 2021
Short summary
Development and field testing of an online instrument for measuring the real-time oxidative potential of ambient particulate matter based on dithiothreitol assay
Joseph V. Puthussery, Chen Zhang, and Vishal Verma
Atmos. Meas. Tech., 11, 5767–5780, https://doi.org/10.5194/amt-11-5767-2018,https://doi.org/10.5194/amt-11-5767-2018, 2018
Short summary
A method for measuring total aerosol oxidative potential (OP) with the dithiothreitol (DTT) assay and comparisons between an urban and roadside site of water-soluble and total OP
Dong Gao, Ting Fang, Vishal Verma, Linghan Zeng, and Rodney J. Weber
Atmos. Meas. Tech., 10, 2821–2835, https://doi.org/10.5194/amt-10-2821-2017,https://doi.org/10.5194/amt-10-2821-2017, 2017
Short summary

Related subject area

Subject: Aerosols | Technique: Laboratory Measurement | Topic: Instruments and Platforms
Exploring non-soluble particles in hailstones through innovative confocal laser and scanning electron microscopy techniques
Anthony C. Bernal Ayala, Angela K. Rowe, Lucia E. Arena, William O. Nachlas, and Maria L. Asar
Atmos. Meas. Tech., 17, 5561–5579, https://doi.org/10.5194/amt-17-5561-2024,https://doi.org/10.5194/amt-17-5561-2024, 2024
Short summary
A comprehensive evaluation of enhanced temperature influence on gas and aerosol chemistry in the lamp-enclosed oxidation flow reactor (OFR) system
Tianle Pan, Andrew T. Lambe, Weiwei Hu, Yicong He, Minghao Hu, Huaishan Zhou, Xinming Wang, Qingqing Hu, Hui Chen, Yue Zhao, Yuanlong Huang, Doug R. Worsnop, Zhe Peng, Melissa A. Morris, Douglas A. Day, Pedro Campuzano-Jost, Jose-Luis Jimenez, and Shantanu H. Jathar
Atmos. Meas. Tech., 17, 4915–4939, https://doi.org/10.5194/amt-17-4915-2024,https://doi.org/10.5194/amt-17-4915-2024, 2024
Short summary
A Novel Methodology for Assessing the Hygroscopicity of Aerosol Filter Samples
Nagendra Raparthi, Anthony S. Wexler, and Ann M. Dillner
EGUsphere, https://doi.org/10.5194/egusphere-2024-2482,https://doi.org/10.5194/egusphere-2024-2482, 2024
Short summary
An oxidation flow reactor for simulating and accelerating secondary aerosol formation in aerosol liquid water and cloud droplets
Ningjin Xu, Chen Le, David R. Cocker, Kunpeng Chen, Ying-Hsuan Lin, and Don R. Collins
Atmos. Meas. Tech., 17, 4227–4243, https://doi.org/10.5194/amt-17-4227-2024,https://doi.org/10.5194/amt-17-4227-2024, 2024
Short summary
Surface equilibrium vapor pressure of organic nanoparticles measured from the dynamic-aerosol-size electrical mobility spectrometer
Ella Häkkinen, Huan Yang, Runlong Cai, and Juha Kangasluoma
Atmos. Meas. Tech., 17, 4211–4225, https://doi.org/10.5194/amt-17-4211-2024,https://doi.org/10.5194/amt-17-4211-2024, 2024
Short summary

Cited articles

Abe, K. and Saito, H: Characterization of t-butyl hydroperoxide toxicity in cultured rat cortical neurones and astrocytes, Pharmacol. Toxicol., 83, 40–46, https://doi.org/10.1111/j.1600-0773.1998.tb01440.x, 1998. 
Alía, M., Ramos, S., Mateos, R., Bravo, L., and Goya, L.: Response of the antioxidant defense system to tert-butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2), J. Biochem. Mol. Toxic., 19, 119–128, https://doi.org/10.1002/jbt.20061, 2005. 
Berg, K. E., Clark, K. M., Li, X., Carter, E. M., Volckens, J., and Henry, C. S.: High-throughput, semi-automated dithiothreitol (DTT) assays for oxidative potential of fine particulate matter, Atmos. Environ., 222, 117132, https://doi.org/10.1016/j.atmosenv.2019.117132, 2020. 
Breitner, S., Peters, A., Zareba, W., Hampel, R., Oakes, D., Wiltshire, J., Frampton, M. W., Hopke, P. K., Cyrys, J., Utell, M. J., Kane, C., Schneider, A., and Rich, D. Q.: Ambient and controlled exposures to particulate air pollution and acute changes in heart rate variability and repolarization, Sci. Rep., 9, 1–12, https://doi.org/10.1038/s41598-019-38531-9, 2019. 
Brown, R. A., Stevanovic, S., Bottle, S., and Ristovski, Z. D.: An instrument for the rapid quantification of PM-bound ROS: the Particle Into Nitroxide Quencher (PINQ), Atmos. Meas. Tech., 12, 23872401, https://doi.org/10.5194/amt-12-2387-2019, 2019. 
Download
Short summary
Oxidative potential (OP) of particulate matter (PM) is an important indicator of PM toxicity. However, no automated instrument has ever been developed to provide a rapid high-throughput analysis of cell-based OP measurements. Here, we developed a semi-automated instrument, the first of its kind, for measuring oxidative potential using rat alveolar cells. We also developed a dataset on the intrinsic cellular OP of several compounds commonly known to be present in ambient PM.